Advances in the Application of Intestinal Flora in Anti-Tumor Therapy

The intestinal flora is the normal microecosystem of the gastrointestinal tract. The intestinal flora has great complexity, and plays crucial roles in nutrient intake, host metabolism, endocrine signaling, homeostasis and immune regulation. Disorders of the intestinal flora can lead to an out-of-balance in immune system, and even results in an increased risk of diseases, including the occurrence and development of malignant tumors. Accumulating evidence shows that intestinal microorganisms may assist in systemic therapy by increasing the “heterogeneity” of the tumor, thereby promoting anti-tumor therapy. Targeting intestinal flora during the treatment of cancer has shown good efficacy. This review focuses on recent advances in research about intestinal flora, the relationship with tumor development, and the potential anti-tumor effects.

Advances in the mechanism of bile acids in liver regeneration

Hepatocytes can divide rapidly and proliferate in the absence of inflammation and fibroplasia in the damaged or partial hepatectomy(PHx)of the liver,which is essential for the recovery of related patients.Recent studies have found that bile acids(BA)play an important role in the process of liver regeneration.In the early stages of PHx,bile acid overload occurs,and liver injury is aggravated by loading.Later bile acids can induce protective and proliferative responses in the liver and promote liver regeneration.In this paper,we summarize the negative effects after bile acid overload and its positive role as a signaling molecule involved in related signaling pathways on liver regeneration,including protection of the liver and promotion of liver regeneration,and its double-edswordged"in Liver regeneration.This provides a theoretical basis for subsequent in-depth study of the mechanism and benefit avoidance in clinical treatment.

Systematic understanding of the underlying target and mechanism of sanguinarine against nasopharyngeal carcinoma through a networkpharmacology approach

Background:Nasopharyngeal carcinoma is a malignant tumor,well known as a cancer type characterized by regional specificity,especially in Southern China.The network pharmacology is an emerging discipline developed in recent years,which has been effectively used to predict the potential therapeutic compounds against disease focusing on the possible therapeutic targets and mechanisms.Sanguinarine,a traditional natural plant-derived phenanthridine alkaloid,has been reported to have a wide variety of pharmacological activities for decades.Methods:In the current study,using the comprehensive network pharmacological method,the potential drug targets of sanguinarine against nasopharyngeal carcinoma were successfully predicted,and verified by molecular docking.The underlying pharmacological mechanism was initially unraveled.Results:Totally,38 potential common targets were confirmed from these potential nasopharyngeal carcinoma therapeutic targets and pharmacological targets of sanguinarine.Their enrichment analyses of GO functions show that protein serine/threonine kinase activity,histone kinase activity,integrin binding,protein tyrosine kinase activity,and cell adhesion molecule binding were top listed.KEGG functional enrichment analysis indicates that the potential pathways are mainly involved into PI3K-Akt signaling pathway.The"drug-target-disease-pathway"network model diagram points out the key genes containing MAPK10,MAPK14,JAK2,BRAF,GSK3B,MET,HSP90AA1,SRC,et al..According to the results of molecular docking,it was further verified that sanguinarine has strong binding ability with MAPK10 and MAPK14.Conclusion:Taken together,this study would provide a clarified theoretical basis for the subsequent wet laboratory research focusing on sanguinarine against nasopharyngeal carcinoma.

Role of triggering receptor expressed on myeloid cells-1 in kidney diseases:A biomarker and potential therapeutic target

Triggering receptor expressed on myeloid cells-1(TREM-1)is a member of the immunoglobulin superfamily.As an amplifier of the inflammatory response,TREM-1 is mainly involved in the production of inflammatory mediators and the regulation of cell survival.TREM-1 has been studied in infectious diseases and more recently in non-infectious disorders.More and more studies have shown that TREM-1 plays an important pathogenic role in kidney diseases.There is evidence that TREM-1 can not only be used as a biomarker for diagnosis of disease but also as a potential therapeutic target to guide the development of novel therapeutic agents for kidney disease.This review summarized molecular biology of TREM-1 and its signaling pathways as well as immune response in the progress of acute kidney injury,renal fibrosis,diabetic nephropathy,immune nephropathy,and renal cell carcinoma.

Knockdown of RFC4 inhibits the cell proliferation of nasopharyngeal carcinoma in vitro and in vivo

Nasopharyngeal carcinoma(NPC)is a malignant tumor that mainly occurs in East and Southeast Asia.Although patients benefit from the main NPC treatments(e.g.,radiotherapy and concurrent chemotherapy),persistent and recurrent diseases still occur in some NPC patients.Therefore,investigating the pathogenesis of NPC is of great clinical significance.In the present study,replication factor c subunit 4(RFC4)is a key potential target involved in NPC progression via bioinformatics analysis.Furthermore,the expression and mechanism of RFC4 in NPC were investigated in vitro and in vivo.Our results revealed that RFC4 was more elevated in NPC tumor tissues than in normal tissues.RFC4 knockdown induced G2/M cell cycle arrest and inhibited NPC cell proliferation in vitro and in vivo.Interestingly,HOXA10 was confirmed as a downstream target of RFC4,and the overexpression of HOXA10 attenuated the silencing of RFC4-induced cell proliferation,colony formation inhibition,and cell cycle arrest.For the first time,this study reveals that RFC4 is required for NPC cell proliferation and may play a pivotal role in NPC tumorigenesis.

A STUDY ON AIRBORNE ALLERGIC POLLEN GRAINS AND SPORES IN NANNING,GUANGXI ZHUANG AUTONOMOUS REGION,CHINA

The rate of respiratory allergic diseases is high. Statitics show that with the people in the United States, the rate of the diseases caused by pollen grains alone is as high as 10％. The rate of the asthmatic bronchitis is 5％ and it often causes complications. The health and work of people have been highly impaired. Pollen grains are the main aspiratory allergens in atmosphere. Therefore, to effectively prevent, diagnose and cure respiratory allergic diseases, we should, first of all,

STRUCTURAL ANALYSIS OF A NEW ABNORMAL HEMOGLOBIN DOUBLE HETEROZYGOTE (α16(A14)Lys→Glu+β26(B8)Glu→Lys)

Two hemoglobin (Hb) variants, one moving fast and another moving slowly, have been found together in a 10-year-old boy of the Zhuang nationality in Tiandeng County, Zhuang Autonomons Region of Guangxi. The relative contents of the varints amount to 9.27% and 27.3% of the total henoglobin, respeetively. Routine hemo. tologic tests were normal The steady of his family showed that his father carried the slowly-moving Hb variant, but his mother, the fast-moving one. This result demonstrated that the genes of