An anion-exchange chromatography method combined solid phase extraction （SPE） was developed for the simultaneous analysis of glycolate acid （GL）, monochloroacetic acid （MCA） and dichloroacetic acid （DCA） in sy...An anion-exchange chromatography method combined solid phase extraction （SPE） was developed for the simultaneous analysis of glycolate acid （GL）, monochloroacetic acid （MCA） and dichloroacetic acid （DCA） in synthetical betaine products. The analytes and unknown anionic impurities were well separated on a Metrosep A supp5 anion-exchange column （150 mm×4 mm） with 2.0 mmol/L Na2CO3＋2.0 mmol/L NaHCO3 solution as eluent. Suppressed conductivity detection was used. A strong cation exchange （SCX） solid phase extraction （SPE） cartridge was used to reduce the concentration of matrix betaine and a Cleanert IC-Ag pretreatment cartridge was used to remove high Cl- concentration. The detection limits of GL, MCA and DCA were 0.09, 0.017 and 0.05 μg/L, respectively. The relative standard deviations （RSDs） of the retention times and peak areas were less than 0.09% and 0.49%, respectively. The recoveries of the three analytes were between 90.6% and 100.8%. The analytical results showed that GL and DCA were present in high concentration and no MCA was found when the proposed ion chromatography method was applied to three synthetical betaine samples. The proposed method is simple, sensitive and timesaving, and is also suitable for routine analysis in quality control of synthetical betaine products.展开更多
A simple,fast,precise and eco-friendly analytical method for the determination of uric acid（UA） in human urine by ion chromatography（IC） was established.The sample pretreatment was not required,only needed centrif...A simple,fast,precise and eco-friendly analytical method for the determination of uric acid（UA） in human urine by ion chromatography（IC） was established.The sample pretreatment was not required,only needed centrifugation and filtration.The separation was carried out on a cation exchange column with 2.0 mmol/L nitric acid as mobile phase at the flow-rate 1.0 mL/min.A non-suppressed conductivity detector was used.The IC analysis time for one run was within 10 min under the optimized IC condition.The detection limits were 0.5μg/L（S/N = 3） for uric acid.The recovery was 100.1%while the relative standard deviation （RSD） was 1.8%from 10 measurements.展开更多
A simple, fast, precise and eco-friendly method, based on ion chromatography （IC） with a suppressed conductivity detector, was proposed for the determination of benzoic acid （BA） in milk in this paper. The chromat...A simple, fast, precise and eco-friendly method, based on ion chromatography （IC） with a suppressed conductivity detector, was proposed for the determination of benzoic acid （BA） in milk in this paper. The chromatographic separation was accomplished by using an anion exchange column eluted with 3.2 mmol/L aqueous Na2C03 and 1.0 mmol/L aqueous NaHCO3 at a flow-rate of 0.7 mL/min. The method validation experiment provided excellent results with respect to linearity （correlation coefficient up to 0.9997）, limit of detection （0.1 ug/L）, recovery values （ranging from 88.0% to 93.0%） and relative standard deviation （RSD） （below 2.2%, n = 7）.展开更多
文摘An anion-exchange chromatography method combined solid phase extraction （SPE） was developed for the simultaneous analysis of glycolate acid （GL）, monochloroacetic acid （MCA） and dichloroacetic acid （DCA） in synthetical betaine products. The analytes and unknown anionic impurities were well separated on a Metrosep A supp5 anion-exchange column （150 mm×4 mm） with 2.0 mmol/L Na2CO3＋2.0 mmol/L NaHCO3 solution as eluent. Suppressed conductivity detection was used. A strong cation exchange （SCX） solid phase extraction （SPE） cartridge was used to reduce the concentration of matrix betaine and a Cleanert IC-Ag pretreatment cartridge was used to remove high Cl- concentration. The detection limits of GL, MCA and DCA were 0.09, 0.017 and 0.05 μg/L, respectively. The relative standard deviations （RSDs） of the retention times and peak areas were less than 0.09% and 0.49%, respectively. The recoveries of the three analytes were between 90.6% and 100.8%. The analytical results showed that GL and DCA were present in high concentration and no MCA was found when the proposed ion chromatography method was applied to three synthetical betaine samples. The proposed method is simple, sensitive and timesaving, and is also suitable for routine analysis in quality control of synthetical betaine products.
文摘A simple,fast,precise and eco-friendly analytical method for the determination of uric acid（UA） in human urine by ion chromatography（IC） was established.The sample pretreatment was not required,only needed centrifugation and filtration.The separation was carried out on a cation exchange column with 2.0 mmol/L nitric acid as mobile phase at the flow-rate 1.0 mL/min.A non-suppressed conductivity detector was used.The IC analysis time for one run was within 10 min under the optimized IC condition.The detection limits were 0.5μg/L（S/N = 3） for uric acid.The recovery was 100.1%while the relative standard deviation （RSD） was 1.8%from 10 measurements.
基金supported by the National Natural Science Fundation of China(Nos.20975056 and 81102411)Natural Science Fundation of Shandong(Nos.ZR2011BZ004 and ZR2011BQ005)+1 种基金JSPS and NSFC under the Japan-China Scientific Cooperation Programme(No.21111140014)the Taishan Scholar Programme of Shandong Province(No.TS20070711),China
文摘A simple, fast, precise and eco-friendly method, based on ion chromatography （IC） with a suppressed conductivity detector, was proposed for the determination of benzoic acid （BA） in milk in this paper. The chromatographic separation was accomplished by using an anion exchange column eluted with 3.2 mmol/L aqueous Na2C03 and 1.0 mmol/L aqueous NaHCO3 at a flow-rate of 0.7 mL/min. The method validation experiment provided excellent results with respect to linearity （correlation coefficient up to 0.9997）, limit of detection （0.1 ug/L）, recovery values （ranging from 88.0% to 93.0%） and relative standard deviation （RSD） （below 2.2%, n = 7）.
