Accumulating evidence suggests that the gut microbiota plays an important role in the pathogenesis of inflammatory bowel disease(IBD).Carnosic acid(CA)is a major antioxidant component of rosemary and sage.Herein,we in...Accumulating evidence suggests that the gut microbiota plays an important role in the pathogenesis of inflammatory bowel disease(IBD).Carnosic acid(CA)is a major antioxidant component of rosemary and sage.Herein,we investigated the protective effects of dietary CA on dextran sodium sulfate(DSS)-induced colitis mouse model with an emphasis on its impact on the composition and metabolic function of gut microbiota.We found that CA effectively attenuated DSS-stimulated colitis in mice,as evidenced by reduced disease activity index(DAI),and systemic and colonic inflammation.Additionally,CA restored microbial diversity and improved the composition of gut microbiota in DSS-treated mice.Moreover,Spearman’s correlation coefficient showed a significant correlation between the fecal metabolites and the gut microbiota species.Changes in gut microbiota and the correlated metabolites might partially explain CA’s anti-inflammatory effects against colitis.Future clinical trials are needed to determine the therapeutic effects and mechanisms of CA on IBD in humans.展开更多
Halite precipitation with water and air temperature was observed in detail,and homogenization temperature of fluid inclusions in halite formed in ancient and modem Chaka Salt Lake was studied.Halite precipitates mainl...Halite precipitation with water and air temperature was observed in detail,and homogenization temperature of fluid inclusions in halite formed in ancient and modem Chaka Salt Lake was studied.Halite precipitates mainly in August every year and largely precipitates between 13 and 15pm at one day when water temperatures reach 20℃but can seldom reach 30℃.Homogenization temperatures of fluid inclusions in halite formed in Chaka Salt Lake range from 14℃to 38℃with an average of 23.7℃.The number of inclusions appears an obvious peak value at homogenization temperatures between 18～25℃,which probably represent the water temperature in which halite mainly precipitates when water temperatures reach 20℃.Therefore,homogenization temperatures of fluid inclusions in halite formed in Chaka Salt Lake can well reflect the water temperature.展开更多
Various combinations of diamond, moissanite, zircon, corundum, rutile and titanitehave been recovered from the Bulqiza chromitites. More than 10 grains of diamond have been recovered, most of which are pale yellow to ...Various combinations of diamond, moissanite, zircon, corundum, rutile and titanitehave been recovered from the Bulqiza chromitites. More than 10 grains of diamond have been recovered, most of which are pale yellow to reddish–orange to colorless. The grains are all 100–300 μm in size and mostly anhedral, but with a range of morphologies including elongated, octahedral and subhedral varieties. Their identification was confirmed by a characteristic shift in the Raman spectra between 1325 cm-1 and 1333 cm-1, mostly at 1331.51 cm-1 or 1326.96 cm-1. This investigation extends the occurrence of diamond and moissanite to the Bulqiza chromitites in the Eastern Mirdita Ophiolite. Integration of the mineralogical, petrological and geochemical data of the Bulqiza chromitites suggests their multi–stage formation. Magnesiochromite grains and perhaps small bodies of chromitite formed at various depths in the upper mantle, and encapsulated the ultra–high pressure, highly reduced and crustal minerals. Some oceanic crustal slabs containing the magnesiochromite and their inclusion were later trapped in suprasubduction zones, where they were modified by tholeiitic and boninitic arc magmas, thus changing the magnesiochromite compositions and depositing chromitite ores in melt channels.展开更多
More and more evidence indicates that organic matter (OM) in immature organic-rich sediments and sedimentary rocks is chemically adsorbed onto the outer surfaces of minerals and into interlayer (inner) surfaces of sme...More and more evidence indicates that organic matter (OM) in immature organic-rich sediments and sedimentary rocks is chemically adsorbed onto the outer surfaces of minerals and into interlayer (inner) surfaces of smectitic clay minerals in the form of amorphous molecular-scale carbon. But there have been few reports about the occurrence of highly mature OM in marine black shales (petroleum source rocks). The occurrence of highly mature OM in the black shales of basal Cambrian from northern Tarim Basin is studied in this paper. Based on the comprehensive analyses of total organic carbon contents (TOC), maximum thermolysis temperatures (T-max) of OM, mineral surface areas (MSA), and scanning electronic microscopic (SEM) and transmission electronic microscopic (TEM) observations of the black shales, it is concluded that the highly mature OM in the marine black shales of the basal Cambrian from northern Tarim Basin occurs in particulates ranging in size from 1 to 5 μm in diameter. Through the contrast of the occurrence of the highly mature OM in the black shales with that of the immature ones in modern marine continental margin sediments, some scientific problems are proposed, which are worth to study further in detail.展开更多
Baculoviruses are used as microbial insecticides, protein expression vectors, epitope display platforms, and most recently as vectors for gene therapy. Understanding the mechanisms that control baculovirus host-range ...Baculoviruses are used as microbial insecticides, protein expression vectors, epitope display platforms, and most recently as vectors for gene therapy. Understanding the mechanisms that control baculovirus host-range and tissue tropisms are important for assessing their safety and for improving their properties for these biotechnology applications. In the past two decades some progress has been made and several baculovirus genes that influence host-range have been identified. Despite this progress, our understanding of the underlying mechanisms that restrict baculovirus host-range is still limited. Here we review what is currently known about baculovirus genes that influence virus host-range.展开更多
South China Sea （SCS） is the largest Western Pacific marginal sea. However, microbial studies have never been performed in the cold seep sediments in the SCS. In 2004, ＂SONNE＂ 177 cruise found two cold seep areas ...South China Sea （SCS） is the largest Western Pacific marginal sea. However, microbial studies have never been performed in the cold seep sediments in the SCS. In 2004, ＂SONNE＂ 177 cruise found two cold seep areas with different water depth in the northern SCS. Haiyang 4 area, where the water depth is around 3000 m, has already been confirmed for active seeping on the seafloor, such as microbial mats, authigenic carbonate crusts and bivalves. We investigated microbial abundance and diver- sity in a 5.55-m sediment core collected from this cold seep area. An integrated approach was employed including geochemistry and 16S rRNA gene phylogenetic analyses. Here, we show that microbial abun- dance and diversity along with geochemistry profiles of the sediment core revealed a coupled reaction between sulphate reduction and methane oxidation. Acridine orange direct count results showed that microbial abundance ranges from 105 to 106 cells/g sediment （wet weight）. The depth-related variation of the abundance showed the same trend as the methane concentration profile. Phylogenetic analysis indicated the presence of sulphate-reducing bacteria and anaerobic methane-oxidizing archaea. The diver- sity was much higher at the surface, but decreased sharply with depth in response to changes in the geochemical conditions of the sediments, such as methane, sulphate concentration and total organic carbon. Marine Benthic Group B, Chloroflexi and JS1 were predominant phylotypes of the archaeal and bacterial libraries, respectively.展开更多
The Rehai Geothermal Field, located in Tengchong County, in central-western Yunnan Prov- ince, is the largest and most intensively studied geothermal field in China. A wide physicochemical diversity of springs （ambie...The Rehai Geothermal Field, located in Tengchong County, in central-western Yunnan Prov- ince, is the largest and most intensively studied geothermal field in China. A wide physicochemical diversity of springs （ambient to -97 ℃; pH from 〈1.8 to≥9.3） provides a multitude of niches for extremophilic microorganisms. A variety of studies have focused on the cultivation, identification, basic physiology, taxonomy, and biotechnological potential of thermophilic microorganisms from Rehai. Ther- mophilic bacteria isolated from Rehai belong to the phyla Firmicutes and Deinococcus-Thermus. Firmicutes include neutrophilic or alkaliphilic Anoxybacillus, Bacillus, Caldalkalibacillus, Caldanaerobacter, Laceyella, and Geobacillus, as well as thermoacidophilic Alicyclobacillus and Sulfobacillus. Isolates from the Deinococcus-Thermus phylum include several Meiothermus and Thermus species. Many of these bacteria synthesize thermostable polymer-degrading enzymes that may be useful for biotech- nology. The thermoacidophilic archaea Acidianus, Metallosphaera, and Sulfolobus have also been isolated and studied. A few studies have reported the isolation of thermophilic viruses belonging to Siphoviridae （TTSP4 and TTSP10） and Fuselloviridae （STSV1） infecting Thermus spp. and Sulfolobus spp., respectively. More recently, cultivation-independent studies using 16S rRNA gene sequences, shotgun metagenomics, or ＂＇functional gene＂ sequences have revealed a mtlch broader diversity of micro- organisms than represented in culture. Studies of the gene and mRNA encoding 113e large subunit of the ammonia monooxygenase （amoA） of ammonia-oxidizing Archaea （AOA） and the tetraether lipid cre- narchaeol, a potential hiomarker for AOA, suggest a wide diversity, but possibly low abundance, of ther- mophilic AOA in Rehai. Finally, we introduce the Tengchong Partnerships in International Research and Education （P1RE） project, an international collaboration between Chinese and U.S. scientists with the goal of promoting international and interdisciplinary cooperation to gain a more holistic and gh〉bal view of life in te^estrial geothermal springs.展开更多
The second IGCP-649 Workshop, held in Agros-Cyprus during 14-20 May 2016, brought together nearly fifty international scientists from around the world, and included a 5-day field excursion on the classic Troodos ophio...The second IGCP-649 Workshop, held in Agros-Cyprus during 14-20 May 2016, brought together nearly fifty international scientists from around the world, and included a 5-day field excursion on the classic Troodos ophiolite. Organized by the IGCP-649 Project Leadership and the Geological Survey Department of Cyprus, the workshop provided a forum for discussions on the latest views and interpretations on the petrogenesis of crustal and upper mantle peridotites in ophiolites, and introduced many young researchers and students to the intemal structure of the classical Troodos ophiolite. This was particularly the case for a large group of Chinese scholars and students, who visited Cyprus and the Troodos ophiolite for the first time. A 4-day profile across the complete ophiolite sequence gave these scientists a first- hand opportunity to examine the lithological and compositional variations within the Cretaceous oceanic crust and to study the igneous and tectonic contacts between them. Lively discussions by the experts and students in front of some of the best 3-dimensional outcrops were most fruitful and allowed all participants to compare the Troodos tectonics with some of ophiolites elsewhere. structure, geochemistry and the other well-documented展开更多
The Yarlung Zangbo Suture Zone（YZSZ）in southern Tibet includes the remnants of Neotethyan oceanic lithosphere and marks a major suture between the Indian Plate to the south and the Lhasa Terrane of Tibet to the nort...The Yarlung Zangbo Suture Zone（YZSZ）in southern Tibet includes the remnants of Neotethyan oceanic lithosphere and marks a major suture between the Indian Plate to the south and the Lhasa Terrane of Tibet to the north（Dupuis et al.,2005;Yang et al.,2011）.In the western part of the YZSZ,the Northern and the Southern sub-belts form two sub-parallel zones of mafic–ultramaficrockassemblageswithoverlapping crystallization ages（Xiong et al.,2011;Hebért et al.,2012;Liu et al.,2015）.The upper mantle section of the Cuobuzha ophiolite in the northern sub-belt of the Yarlung–Zangbo Suture Zone（YZSZ）in SW Tibet comprises mainly clinopyroxene（cpx）–rich and depleted harzburgites.Spinels in the cpx-harzburgites show lower Cr#values（12.6–15.1）than the spinels in the harzburgites（26.1–34.5）,and the cpx-harzburgites display higher heavy rare earth element concentrations than the depleted harzburgites.The harzburgites have subchondritic Os isotopic compositions（0.11624–0.11699）,whereas the cpx-harzburgites have suprachondritic 187Os/188Os ratios（0.12831–0.13125）with higher Re concentrations（0.380-0.575 ppb）.The cpx-harzburgites plot in a Re vs.Al2O3 diagram as a result of subsequent addition of Re following the last partial melting event that occurred during mid-ocean ridge melt evolution processes（Uysal et al.,2015）.Although these geochemical and isotopic signatures suggest that both peridotite types in the ophiolite represent mid-ocean ridge type upper mantle units,their melt evolution trends reflect different mantle processes.The cpx-harzburgites formed from low-degree partial melting（;%）of a primitive mantle source,and they weresubsequently modified by melt–rock interactions in a mid-ocean ridge environment.The depleted harzburgites,on the other hand,were produced by re-melting of the cpx-harzburgites,which later interacted with MORB-or island arc tholeiite（IAT）-like melts（Fig.1）possibly in a trench-distal backarc spreading center.Our new isotopic and geochemical data from the Cuobuzha peridotites confirm that the Neotethyan upper mantle had highly heterogeneous Os isotopic compositions as a result of multiple melt production and melt extraction events during its seafloor spreading evolution.展开更多
基金supported by Natural Science Foundation of Guangdong basic and applied basic research foundation(2021A1515010965)General project of Basic and applied basic Research in Guangzhou(202102080241)+3 种基金Laboratory opening project of Guangzhou Medical University(PX-1020423)Natural Science Foundation of Guangdong basic and applied basic research foundation([2018]105)Guangdong Provincial Department of Education(S202010570042)Communist Youth League Committee of Guangzhou Medical University(2019A060).
文摘Accumulating evidence suggests that the gut microbiota plays an important role in the pathogenesis of inflammatory bowel disease(IBD).Carnosic acid(CA)is a major antioxidant component of rosemary and sage.Herein,we investigated the protective effects of dietary CA on dextran sodium sulfate(DSS)-induced colitis mouse model with an emphasis on its impact on the composition and metabolic function of gut microbiota.We found that CA effectively attenuated DSS-stimulated colitis in mice,as evidenced by reduced disease activity index(DAI),and systemic and colonic inflammation.Additionally,CA restored microbial diversity and improved the composition of gut microbiota in DSS-treated mice.Moreover,Spearman’s correlation coefficient showed a significant correlation between the fecal metabolites and the gut microbiota species.Changes in gut microbiota and the correlated metabolites might partially explain CA’s anti-inflammatory effects against colitis.Future clinical trials are needed to determine the therapeutic effects and mechanisms of CA on IBD in humans.
基金National Natural Science Foundation of China （Grant No.40373016）.
文摘Halite precipitation with water and air temperature was observed in detail,and homogenization temperature of fluid inclusions in halite formed in ancient and modem Chaka Salt Lake was studied.Halite precipitates mainly in August every year and largely precipitates between 13 and 15pm at one day when water temperatures reach 20℃but can seldom reach 30℃.Homogenization temperatures of fluid inclusions in halite formed in Chaka Salt Lake range from 14℃to 38℃with an average of 23.7℃.The number of inclusions appears an obvious peak value at homogenization temperatures between 18～25℃,which probably represent the water temperature in which halite mainly precipitates when water temperatures reach 20℃.Therefore,homogenization temperatures of fluid inclusions in halite formed in Chaka Salt Lake can well reflect the water temperature.
基金funded by grants from the NSF China(Nos.41502062,41672046,41541017,41641015)the China Geological Survey(Nos.DD20160023-01,201511022)+2 种基金National Key Research and Development Project of China(2016YFC0600310)from the Institute of Geology,Chinese Academy of Geological Sciences(J1526)IGCP–649
文摘Various combinations of diamond, moissanite, zircon, corundum, rutile and titanitehave been recovered from the Bulqiza chromitites. More than 10 grains of diamond have been recovered, most of which are pale yellow to reddish–orange to colorless. The grains are all 100–300 μm in size and mostly anhedral, but with a range of morphologies including elongated, octahedral and subhedral varieties. Their identification was confirmed by a characteristic shift in the Raman spectra between 1325 cm-1 and 1333 cm-1, mostly at 1331.51 cm-1 or 1326.96 cm-1. This investigation extends the occurrence of diamond and moissanite to the Bulqiza chromitites in the Eastern Mirdita Ophiolite. Integration of the mineralogical, petrological and geochemical data of the Bulqiza chromitites suggests their multi–stage formation. Magnesiochromite grains and perhaps small bodies of chromitite formed at various depths in the upper mantle, and encapsulated the ultra–high pressure, highly reduced and crustal minerals. Some oceanic crustal slabs containing the magnesiochromite and their inclusion were later trapped in suprasubduction zones, where they were modified by tholeiitic and boninitic arc magmas, thus changing the magnesiochromite compositions and depositing chromitite ores in melt channels.
文摘More and more evidence indicates that organic matter (OM) in immature organic-rich sediments and sedimentary rocks is chemically adsorbed onto the outer surfaces of minerals and into interlayer (inner) surfaces of smectitic clay minerals in the form of amorphous molecular-scale carbon. But there have been few reports about the occurrence of highly mature OM in marine black shales (petroleum source rocks). The occurrence of highly mature OM in the black shales of basal Cambrian from northern Tarim Basin is studied in this paper. Based on the comprehensive analyses of total organic carbon contents (TOC), maximum thermolysis temperatures (T-max) of OM, mineral surface areas (MSA), and scanning electronic microscopic (SEM) and transmission electronic microscopic (TEM) observations of the black shales, it is concluded that the highly mature OM in the marine black shales of the basal Cambrian from northern Tarim Basin occurs in particulates ranging in size from 1 to 5 μm in diameter. Through the contrast of the occurrence of the highly mature OM in the black shales with that of the immature ones in modern marine continental margin sediments, some scientific problems are proposed, which are worth to study further in detail.
文摘Baculoviruses are used as microbial insecticides, protein expression vectors, epitope display platforms, and most recently as vectors for gene therapy. Understanding the mechanisms that control baculovirus host-range and tissue tropisms are important for assessing their safety and for improving their properties for these biotechnology applications. In the past two decades some progress has been made and several baculovirus genes that influence host-range have been identified. Despite this progress, our understanding of the underlying mechanisms that restrict baculovirus host-range is still limited. Here we review what is currently known about baculovirus genes that influence virus host-range.
基金supported by the National Program on Key Basic Research Project(973 Program)(Grant No.2009CB219502)National Special Foundation(Grant No.GZH200200203-02-01)Non-profit Industry Financial Program of Ministry of Land and Resources of the PRC(Grant No.200811014-02)
文摘South China Sea （SCS） is the largest Western Pacific marginal sea. However, microbial studies have never been performed in the cold seep sediments in the SCS. In 2004, ＂SONNE＂ 177 cruise found two cold seep areas with different water depth in the northern SCS. Haiyang 4 area, where the water depth is around 3000 m, has already been confirmed for active seeping on the seafloor, such as microbial mats, authigenic carbonate crusts and bivalves. We investigated microbial abundance and diver- sity in a 5.55-m sediment core collected from this cold seep area. An integrated approach was employed including geochemistry and 16S rRNA gene phylogenetic analyses. Here, we show that microbial abun- dance and diversity along with geochemistry profiles of the sediment core revealed a coupled reaction between sulphate reduction and methane oxidation. Acridine orange direct count results showed that microbial abundance ranges from 105 to 106 cells/g sediment （wet weight）. The depth-related variation of the abundance showed the same trend as the methane concentration profile. Phylogenetic analysis indicated the presence of sulphate-reducing bacteria and anaerobic methane-oxidizing archaea. The diver- sity was much higher at the surface, but decreased sharply with depth in response to changes in the geochemical conditions of the sediments, such as methane, sulphate concentration and total organic carbon. Marine Benthic Group B, Chloroflexi and JS1 were predominant phylotypes of the archaeal and bacterial libraries, respectively.
基金supported generously by the U.S.National Science Foundation(Grant Nos.MCB-0546865 and OISE- 0968421 & OISE-0836450)National Natural Science Foundation of China(Grant No.31070007)
文摘The Rehai Geothermal Field, located in Tengchong County, in central-western Yunnan Prov- ince, is the largest and most intensively studied geothermal field in China. A wide physicochemical diversity of springs （ambient to -97 ℃; pH from 〈1.8 to≥9.3） provides a multitude of niches for extremophilic microorganisms. A variety of studies have focused on the cultivation, identification, basic physiology, taxonomy, and biotechnological potential of thermophilic microorganisms from Rehai. Ther- mophilic bacteria isolated from Rehai belong to the phyla Firmicutes and Deinococcus-Thermus. Firmicutes include neutrophilic or alkaliphilic Anoxybacillus, Bacillus, Caldalkalibacillus, Caldanaerobacter, Laceyella, and Geobacillus, as well as thermoacidophilic Alicyclobacillus and Sulfobacillus. Isolates from the Deinococcus-Thermus phylum include several Meiothermus and Thermus species. Many of these bacteria synthesize thermostable polymer-degrading enzymes that may be useful for biotech- nology. The thermoacidophilic archaea Acidianus, Metallosphaera, and Sulfolobus have also been isolated and studied. A few studies have reported the isolation of thermophilic viruses belonging to Siphoviridae （TTSP4 and TTSP10） and Fuselloviridae （STSV1） infecting Thermus spp. and Sulfolobus spp., respectively. More recently, cultivation-independent studies using 16S rRNA gene sequences, shotgun metagenomics, or ＂＇functional gene＂ sequences have revealed a mtlch broader diversity of micro- organisms than represented in culture. Studies of the gene and mRNA encoding 113e large subunit of the ammonia monooxygenase （amoA） of ammonia-oxidizing Archaea （AOA） and the tetraether lipid cre- narchaeol, a potential hiomarker for AOA, suggest a wide diversity, but possibly low abundance, of ther- mophilic AOA in Rehai. Finally, we introduce the Tengchong Partnerships in International Research and Education （P1RE） project, an international collaboration between Chinese and U.S. scientists with the goal of promoting international and interdisciplinary cooperation to gain a more holistic and gh〉bal view of life in te^estrial geothermal springs.
文摘The second IGCP-649 Workshop, held in Agros-Cyprus during 14-20 May 2016, brought together nearly fifty international scientists from around the world, and included a 5-day field excursion on the classic Troodos ophiolite. Organized by the IGCP-649 Project Leadership and the Geological Survey Department of Cyprus, the workshop provided a forum for discussions on the latest views and interpretations on the petrogenesis of crustal and upper mantle peridotites in ophiolites, and introduced many young researchers and students to the intemal structure of the classical Troodos ophiolite. This was particularly the case for a large group of Chinese scholars and students, who visited Cyprus and the Troodos ophiolite for the first time. A 4-day profile across the complete ophiolite sequence gave these scientists a first- hand opportunity to examine the lithological and compositional variations within the Cretaceous oceanic crust and to study the igneous and tectonic contacts between them. Lively discussions by the experts and students in front of some of the best 3-dimensional outcrops were most fruitful and allowed all participants to compare the Troodos tectonics with some of ophiolites elsewhere. structure, geochemistry and the other well-documented
文摘The Yarlung Zangbo Suture Zone（YZSZ）in southern Tibet includes the remnants of Neotethyan oceanic lithosphere and marks a major suture between the Indian Plate to the south and the Lhasa Terrane of Tibet to the north（Dupuis et al.,2005;Yang et al.,2011）.In the western part of the YZSZ,the Northern and the Southern sub-belts form two sub-parallel zones of mafic–ultramaficrockassemblageswithoverlapping crystallization ages（Xiong et al.,2011;Hebért et al.,2012;Liu et al.,2015）.The upper mantle section of the Cuobuzha ophiolite in the northern sub-belt of the Yarlung–Zangbo Suture Zone（YZSZ）in SW Tibet comprises mainly clinopyroxene（cpx）–rich and depleted harzburgites.Spinels in the cpx-harzburgites show lower Cr#values（12.6–15.1）than the spinels in the harzburgites（26.1–34.5）,and the cpx-harzburgites display higher heavy rare earth element concentrations than the depleted harzburgites.The harzburgites have subchondritic Os isotopic compositions（0.11624–0.11699）,whereas the cpx-harzburgites have suprachondritic 187Os/188Os ratios（0.12831–0.13125）with higher Re concentrations（0.380-0.575 ppb）.The cpx-harzburgites plot in a Re vs.Al2O3 diagram as a result of subsequent addition of Re following the last partial melting event that occurred during mid-ocean ridge melt evolution processes（Uysal et al.,2015）.Although these geochemical and isotopic signatures suggest that both peridotite types in the ophiolite represent mid-ocean ridge type upper mantle units,their melt evolution trends reflect different mantle processes.The cpx-harzburgites formed from low-degree partial melting（;%）of a primitive mantle source,and they weresubsequently modified by melt–rock interactions in a mid-ocean ridge environment.The depleted harzburgites,on the other hand,were produced by re-melting of the cpx-harzburgites,which later interacted with MORB-or island arc tholeiite（IAT）-like melts（Fig.1）possibly in a trench-distal backarc spreading center.Our new isotopic and geochemical data from the Cuobuzha peridotites confirm that the Neotethyan upper mantle had highly heterogeneous Os isotopic compositions as a result of multiple melt production and melt extraction events during its seafloor spreading evolution.
