The need for the bacterial proteinase is rapidly growing,urging to catch a lowcost medium for the microbial fermentation,nanoparticles can play a vital role in this respect.The proteinase of Talaromyces purpureogenus ...The need for the bacterial proteinase is rapidly growing,urging to catch a lowcost medium for the microbial fermentation,nanoparticles can play a vital role in this respect.The proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation process.The interface amongst nitrogen sources(NH_(4)Cl and yeast extract)was investigated,applying the statistical modeling of central composite design under solid-state fermentation.The optimum medium for proteinase secretion was stimulated by 979.82 mg NH_(4)Cl and 437.68 mg yeast extract per 100 g substrate,yielding 108.15 U/g tubers.Using Plackett-Burman experimental design,the nanoparticles Co,Ni and Fe were assessed as inducers for proteinase stimulants.Co nanoparticles(5 ppm)were the greatest in both proteinase production by the fungus as well as an inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the reaction mixture,liberating the extreme quantity of amino acids,compared with the lack of the nanoparticles.The findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during the degradation of proteinaceous materials induce proteinase catalyst.This approach could be extended to modulate the productivity and activity of similar biomolecules.展开更多
Because of its antitumor therapeutic-activity,as well as its application in food industries to improve the quality,L-asparaginase has attracted considerable attention from several investigators.In recent years,fungi h...Because of its antitumor therapeutic-activity,as well as its application in food industries to improve the quality,L-asparaginase has attracted considerable attention from several investigators.In recent years,fungi have occupied advanced rank among microorganisms in the production process of the enzyme.This review is spotting the light on the advantages of fungal enzyme and its applications in the food industry and medications.The solid-state fermentation was discussed as the wide alternative and most accepted biosynthesis technique.However,some lights were also spotted to the statistical experimental design of the fermentation process,mainly on the methodology of the response surface for L-asparaginase biosynthesis by fungi.Finally,the immobilization of the enzyme and the features of the widely used solid substrates for the maximization of the production process were explored.展开更多
Profound inspection of the life forms on the earth teaches how to be the complexity of interrelationships among the various systems.Because of the emergence of novel viruses all the time and the inadequate of vaccines...Profound inspection of the life forms on the earth teaches how to be the complexity of interrelationships among the various systems.Because of the emergence of novel viruses all the time and the inadequate of vaccines and antivirals,viral contagions are amongst the most causative diseases affecting people worldwide.Fungi exemplify a massive source of bioactive molecules as,many fungal secondary metabolities like Oxoglyantrypine,Carneic acid F,Scedapin C,Asteltoxin E,Phomanolide,Norquinadoline A and Quinadoline B have antiviral activity.This review deals with how secondary metabolites of fungi can help in the war against viruses in general and especially Coronaviruses moreover several pieces of literature pointed out that many clusters of fungi in different biotopes are waiting to be exploited.展开更多
基金would like to thank the Deanship of Scientific Research at King Khalid University,Abha,Kingdom of Saudi Arabia for funding this work under grant no.(R.G.P.1/75/40).
文摘The need for the bacterial proteinase is rapidly growing,urging to catch a lowcost medium for the microbial fermentation,nanoparticles can play a vital role in this respect.The proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation process.The interface amongst nitrogen sources(NH_(4)Cl and yeast extract)was investigated,applying the statistical modeling of central composite design under solid-state fermentation.The optimum medium for proteinase secretion was stimulated by 979.82 mg NH_(4)Cl and 437.68 mg yeast extract per 100 g substrate,yielding 108.15 U/g tubers.Using Plackett-Burman experimental design,the nanoparticles Co,Ni and Fe were assessed as inducers for proteinase stimulants.Co nanoparticles(5 ppm)were the greatest in both proteinase production by the fungus as well as an inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the reaction mixture,liberating the extreme quantity of amino acids,compared with the lack of the nanoparticles.The findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during the degradation of proteinaceous materials induce proteinase catalyst.This approach could be extended to modulate the productivity and activity of similar biomolecules.
基金the Deanship of Scientific Research at King Khalid University,Abha,KSA for funding this work under Grant number(G.R.P/150/40).
文摘Because of its antitumor therapeutic-activity,as well as its application in food industries to improve the quality,L-asparaginase has attracted considerable attention from several investigators.In recent years,fungi have occupied advanced rank among microorganisms in the production process of the enzyme.This review is spotting the light on the advantages of fungal enzyme and its applications in the food industry and medications.The solid-state fermentation was discussed as the wide alternative and most accepted biosynthesis technique.However,some lights were also spotted to the statistical experimental design of the fermentation process,mainly on the methodology of the response surface for L-asparaginase biosynthesis by fungi.Finally,the immobilization of the enzyme and the features of the widely used solid substrates for the maximization of the production process were explored.
基金The authors are thankful to the Institute of Research and Consulting Studies at King Khalid University for supporting this research through Grant No.#6–93–S–2020.
文摘Profound inspection of the life forms on the earth teaches how to be the complexity of interrelationships among the various systems.Because of the emergence of novel viruses all the time and the inadequate of vaccines and antivirals,viral contagions are amongst the most causative diseases affecting people worldwide.Fungi exemplify a massive source of bioactive molecules as,many fungal secondary metabolities like Oxoglyantrypine,Carneic acid F,Scedapin C,Asteltoxin E,Phomanolide,Norquinadoline A and Quinadoline B have antiviral activity.This review deals with how secondary metabolites of fungi can help in the war against viruses in general and especially Coronaviruses moreover several pieces of literature pointed out that many clusters of fungi in different biotopes are waiting to be exploited.
