Anthracnose disease caused by the Colletotrichum gloeosporioides species complex is a major problem worldwide.In this study,we investigated the phylogenetic diversity of 207 Indian Colletotrichum isolates,associated w...Anthracnose disease caused by the Colletotrichum gloeosporioides species complex is a major problem worldwide.In this study,we investigated the phylogenetic diversity of 207 Indian Colletotrichum isolates,associated with symptomatic and asymptomatic tissues of mango,belonging to this species complex.Phylogenetic analyses were performed based on a 6-gene dataset(act,cal,chs1,gapdh,ITS and tub2),followed by ApMat sequence-analysis.The ApMat-based phylogeny was found to be superior as it provided finer resolution in most of the species-level clades.Importantly,the ApMat marker identified seven lineages within C.siamense sensu lato,including C.jasmini-sambac,C.hymenocallidis,C.melanocaulon,C.siamense sensu stricto and three undesignated,potentially novel lineages.In this study,C.fragariae sensu stricto,C.fructicola,C.jasmini-sambac,C.melanocaulon and five undesignated,potentially novel lineages were found to be associated with mango tissues.There is a need to develop a consensus among mycologists as to which genes should be used to define and delimit a Colletotrichum species and in the mean time mycologists should voluntarily restrain from describing new species based on inadequate datasets.展开更多
Colletotrichum gloeosporioides sensu lato has been associated with anthracnose in diverse commercial crops.It is now established that C.gloeosporioides sensu lato comprises 33 phylogenetic species and C.gloeosporioide...Colletotrichum gloeosporioides sensu lato has been associated with anthracnose in diverse commercial crops.It is now established that C.gloeosporioides sensu lato comprises 33 phylogenetic species and C.gloeosporioides sensu stricto is not a common pathogen of tropical fruits.In this study,we investigated the phylogenetic relationships of 85 Colletotrichum isolates associated with select tropical fruits and flowering plants from India.In the ApMat marker analysis,the 85 isolates clustered with 7 known Colletotrichum species(C.aotearoa,C.dianesei,C.endomangiferae,C.musae,C.siamense,C.theobromicola,Glomerella cingulata f.sp.camelliae)and six novel lineages.One of the novel lineages is described and illustrated in this paper as Colletotrichum communis sp.nov.,while new-host pathogen associations for C.aotearoa,C.endomangiferae,C.dianesei and C.theobromicola are reported from India.Out of the 85 isolates analysed in this paper,73 isolates clustered within the C.siamense species complex,indicating that C.siamense species complex,not C.gloeosporioides sensu stricto,is common on tropical fruits.In comparison with act,cal,gapdh,ITS and tub2 gene markers,we recommend the use of the ApMat marker for accurate identification of cryptic species within the C.siamense species complex.We believe that the ApMat marker,in combination with one or two similar‘phylogenetically superior’gene markers,is a better candidate for specieslevel classification of fungi that were traditionally identified as‘Colletotrichum gloeosporioides’.展开更多
基金We thank Institute of Microbial Technology(CSIR-IMTECH)for the financial supportsupported by CSIR-IMTECH-OLP0071 project and CSIR-SRF fellowship awarded to GS and UGC-SRF fellowship to NK.K.D.+1 种基金yde thanks the National Research Council of Thailand for the award of grant No.54201020003a grant from the National Plan of Science and Technology,King Abdulaziz City of Science and Technology,Riyadh,Saudi Arabia,project No.10-Bio-965-02 to study Colletotrichum.
文摘Anthracnose disease caused by the Colletotrichum gloeosporioides species complex is a major problem worldwide.In this study,we investigated the phylogenetic diversity of 207 Indian Colletotrichum isolates,associated with symptomatic and asymptomatic tissues of mango,belonging to this species complex.Phylogenetic analyses were performed based on a 6-gene dataset(act,cal,chs1,gapdh,ITS and tub2),followed by ApMat sequence-analysis.The ApMat-based phylogeny was found to be superior as it provided finer resolution in most of the species-level clades.Importantly,the ApMat marker identified seven lineages within C.siamense sensu lato,including C.jasmini-sambac,C.hymenocallidis,C.melanocaulon,C.siamense sensu stricto and three undesignated,potentially novel lineages.In this study,C.fragariae sensu stricto,C.fructicola,C.jasmini-sambac,C.melanocaulon and five undesignated,potentially novel lineages were found to be associated with mango tissues.There is a need to develop a consensus among mycologists as to which genes should be used to define and delimit a Colletotrichum species and in the mean time mycologists should voluntarily restrain from describing new species based on inadequate datasets.
基金The authors would like to thank CSIR-Institute of Microbial Technology,Chandigarh for the financial support,Dr.D.Ananthapadmanaban for his help in the microscopy and Mr.Deepak Bhatt for DNA sequencing assistance.Drs.Kevin D.Hyde,Lei Cai and Bevan Weir are thanked for the inspiration and useful discussions on Colletotrichum taxonomy.This work was supported by IMTECHOLP0071 project and CSIR-SRF fellowship awarded to GS.This is NIO contribution no.7636 and IMTECH communication no.IMT2014/21.
文摘Colletotrichum gloeosporioides sensu lato has been associated with anthracnose in diverse commercial crops.It is now established that C.gloeosporioides sensu lato comprises 33 phylogenetic species and C.gloeosporioides sensu stricto is not a common pathogen of tropical fruits.In this study,we investigated the phylogenetic relationships of 85 Colletotrichum isolates associated with select tropical fruits and flowering plants from India.In the ApMat marker analysis,the 85 isolates clustered with 7 known Colletotrichum species(C.aotearoa,C.dianesei,C.endomangiferae,C.musae,C.siamense,C.theobromicola,Glomerella cingulata f.sp.camelliae)and six novel lineages.One of the novel lineages is described and illustrated in this paper as Colletotrichum communis sp.nov.,while new-host pathogen associations for C.aotearoa,C.endomangiferae,C.dianesei and C.theobromicola are reported from India.Out of the 85 isolates analysed in this paper,73 isolates clustered within the C.siamense species complex,indicating that C.siamense species complex,not C.gloeosporioides sensu stricto,is common on tropical fruits.In comparison with act,cal,gapdh,ITS and tub2 gene markers,we recommend the use of the ApMat marker for accurate identification of cryptic species within the C.siamense species complex.We believe that the ApMat marker,in combination with one or two similar‘phylogenetically superior’gene markers,is a better candidate for specieslevel classification of fungi that were traditionally identified as‘Colletotrichum gloeosporioides’.
