Many efforts have been devoted to studying the inhibitory effect of Salvadora persica (Miswak) on oral microorganisms. However, information regarding its antibacterial activity against other human pathogens is rare. P...Many efforts have been devoted to studying the inhibitory effect of Salvadora persica (Miswak) on oral microorganisms. However, information regarding its antibacterial activity against other human pathogens is rare. Particularly, against Pseudomonas aeruginosa, Acinetobacter baumannii and Enterobacter cloacae. The purpose of this piece of work was to evaluate the antioxidant power and in vitro antibacterial activities of Salvadora persica extract against the three bacterial clinical isolates mentioned above. Therefore, the action of Salvadora persica extract on the three germs, studied via the aromatograms method, has given an inhibition zone 20, 18 and 14 respectively on Pseudomonas aeruginosa, Acinetobacter baumannii and Enterobacter cloacae. The methanol extract of Salvadora persica using Soxhlet has given an output of 19%. The antioxidant evaluation, which was performed using the DPPH (1,1-diphenyl-2-picryl-hydrazyl), free radical scavenging method, indicated that the methanol extract showed good antioxidant efficiency (IC50 = 15.47 mg/ml) compared to ascorbic acid with (IC50 = 0.097 mg/ml).展开更多
The minimum inhibitory concentration (MIC) is the concentration at which an antibacterial agent experiences the complete inhibition of organism growth. Bacteriophages represent a rich and unique resource of anti-infec...The minimum inhibitory concentration (MIC) is the concentration at which an antibacterial agent experiences the complete inhibition of organism growth. Bacteriophages represent a rich and unique resource of anti-infectives to counter the growing world-wide problem of antibiotic resistance. In this study, we compared the host range of lytic bacteriophages and temperate phagesbelonging to various genera, namely Staphylococcus, E. coli and Salmonella, with a range of clinical isolates using two methods: the classical agar overlay method and a newly developed MIC method. MIC was only observed with isolates that were susceptible to phage infection, which correlated with the agar overlay assay, whereas no MIC was detected with isolates that were resistant to phage infection. The simple MIC method was useful in determining phage adsorption and host range, and detecting possible prophage contamination in phage preparations. Interestingly, this method was also applicable to strain differentiation through phage susceptibility testing using a 96-well, high throughput format that proved to be easy, cost-effective, fast and reliable.展开更多
After ban of antibiotics growth promoters (AGP) in Europe in 2006, use of non-medicated gut microbiota regulators as feed additives has dramatically increased. This study aimed at describing the effects of a copper-ex...After ban of antibiotics growth promoters (AGP) in Europe in 2006, use of non-medicated gut microbiota regulators as feed additives has dramatically increased. This study aimed at describing the effects of a copper-exchanged zeolite on broiler growth performance, small intestine morphology and microbiota composition. Illumina Sequencing of the V3-V4 region of the 16S rRNA gene was employed to study the small intestine microbiota. This microbiota with copper-exchanged zeolite treated-chickens was significantly less diverse with an almost exclusive presence of Lactobacillus johnsonii and Lactobacillus reuteri. These Lactobacilli are correlated with increased diameter, length and weight of the three segments of the small intestine and decreased viscosity of the intestinal content, suggesting probiotic action. The tested copper-exchanged zeolite would act as a prebiotic, selecting a “favorable” flora for the healthy broilers development.展开更多
文摘Many efforts have been devoted to studying the inhibitory effect of Salvadora persica (Miswak) on oral microorganisms. However, information regarding its antibacterial activity against other human pathogens is rare. Particularly, against Pseudomonas aeruginosa, Acinetobacter baumannii and Enterobacter cloacae. The purpose of this piece of work was to evaluate the antioxidant power and in vitro antibacterial activities of Salvadora persica extract against the three bacterial clinical isolates mentioned above. Therefore, the action of Salvadora persica extract on the three germs, studied via the aromatograms method, has given an inhibition zone 20, 18 and 14 respectively on Pseudomonas aeruginosa, Acinetobacter baumannii and Enterobacter cloacae. The methanol extract of Salvadora persica using Soxhlet has given an output of 19%. The antioxidant evaluation, which was performed using the DPPH (1,1-diphenyl-2-picryl-hydrazyl), free radical scavenging method, indicated that the methanol extract showed good antioxidant efficiency (IC50 = 15.47 mg/ml) compared to ascorbic acid with (IC50 = 0.097 mg/ml).
文摘The minimum inhibitory concentration (MIC) is the concentration at which an antibacterial agent experiences the complete inhibition of organism growth. Bacteriophages represent a rich and unique resource of anti-infectives to counter the growing world-wide problem of antibiotic resistance. In this study, we compared the host range of lytic bacteriophages and temperate phagesbelonging to various genera, namely Staphylococcus, E. coli and Salmonella, with a range of clinical isolates using two methods: the classical agar overlay method and a newly developed MIC method. MIC was only observed with isolates that were susceptible to phage infection, which correlated with the agar overlay assay, whereas no MIC was detected with isolates that were resistant to phage infection. The simple MIC method was useful in determining phage adsorption and host range, and detecting possible prophage contamination in phage preparations. Interestingly, this method was also applicable to strain differentiation through phage susceptibility testing using a 96-well, high throughput format that proved to be easy, cost-effective, fast and reliable.
文摘After ban of antibiotics growth promoters (AGP) in Europe in 2006, use of non-medicated gut microbiota regulators as feed additives has dramatically increased. This study aimed at describing the effects of a copper-exchanged zeolite on broiler growth performance, small intestine morphology and microbiota composition. Illumina Sequencing of the V3-V4 region of the 16S rRNA gene was employed to study the small intestine microbiota. This microbiota with copper-exchanged zeolite treated-chickens was significantly less diverse with an almost exclusive presence of Lactobacillus johnsonii and Lactobacillus reuteri. These Lactobacilli are correlated with increased diameter, length and weight of the three segments of the small intestine and decreased viscosity of the intestinal content, suggesting probiotic action. The tested copper-exchanged zeolite would act as a prebiotic, selecting a “favorable” flora for the healthy broilers development.
