Six genes encoding light-harvesting complex (LHC) protein have been characterized in the multicellular red alga Griffithsia japonica EST analysis. Three of them were full sequences while others were partial sequences ...Six genes encoding light-harvesting complex (LHC) protein have been characterized in the multicellular red alga Griffithsia japonica EST analysis. Three of them were full sequences while others were partial sequences with 3'-UTRs. The cleavage sites between signal peptide and mature LHC protein were analyzed on these three full sequences. The sequence characteristics, calculated molecular weights and isoelectric point (pI) values and hydrophobicity of the mature proteins were deduced and analyzed. Comparing the LHC sequences of G. japonica with higher plant, Chlorophyta, chromophytes and other red algae, the high conservation of the chlorophyll (Chl) binding site among chromophytes and red algae were revealed. Phylogenetic analysis on LHC proteins from higher plant, green algae, euglena, brown algae, diatom, cryptomonad, Raphidophyte and red algae reveals that (1) there are two distinct groups of Chl a/b and Chl a/c -binding LHC; (2) Chl a binding proteins of red algae share greater similarities with the Chl a/c-binding proteins of the chromophytes and dinoflagellate than with the Chl a/b - binding proteins of the green algae and higher plants; (3) chromophyte's LHC is supposed to be evolved from red algae LHC.展开更多
Background Human metapneumovirus （hMPV） was discovered by scientists in the Netherlands as a novel respiratory virus in 2001 and had been found in children with acute respiratory tract infections （ARTI） in China. ...Background Human metapneumovirus （hMPV） was discovered by scientists in the Netherlands as a novel respiratory virus in 2001 and had been found in children with acute respiratory tract infections （ARTI） in China. The objective of this study was to determine the importance of hMPV infection in children in Beijing and the genotypes of the circulating virus by the surveillance during a four-consecutive-year period.展开更多
A54-year-old male smoker was admitted to our .hospital after undergoing the drainage of a leftperitonsillar abscess. Ten days prior to admission （day 1）, he complained of a ＂scratchy＂ throat with a fever of 38.5℃...A54-year-old male smoker was admitted to our .hospital after undergoing the drainage of a leftperitonsillar abscess. Ten days prior to admission （day 1）, he complained of a ＂scratchy＂ throat with a fever of 38.5℃. He had an enlarged left tonsil with prominent exudate; white blood cell count （WCC） 8.8×109/L; platelet count （Plt） 103 × 109/L; chest X-ray was normal in a medical clinic. Then the patient received an intravenous injection of 4 million units of penicillin G every 6 hours and 500 mg of oral azithromycin once daily. But his condition worsened with a temperature of 41℃ and laryngoscopy confirmed a marked left-sided peritonsillar abscess which was drained. On day 10, the patient was transferred to our hospital after developing persistent fever, rigors, left-sided neck pain, pain on swallowing and purulent bloody sputum.展开更多
文摘Six genes encoding light-harvesting complex (LHC) protein have been characterized in the multicellular red alga Griffithsia japonica EST analysis. Three of them were full sequences while others were partial sequences with 3'-UTRs. The cleavage sites between signal peptide and mature LHC protein were analyzed on these three full sequences. The sequence characteristics, calculated molecular weights and isoelectric point (pI) values and hydrophobicity of the mature proteins were deduced and analyzed. Comparing the LHC sequences of G. japonica with higher plant, Chlorophyta, chromophytes and other red algae, the high conservation of the chlorophyll (Chl) binding site among chromophytes and red algae were revealed. Phylogenetic analysis on LHC proteins from higher plant, green algae, euglena, brown algae, diatom, cryptomonad, Raphidophyte and red algae reveals that (1) there are two distinct groups of Chl a/b and Chl a/c -binding LHC; (2) Chl a binding proteins of red algae share greater similarities with the Chl a/c-binding proteins of the chromophytes and dinoflagellate than with the Chl a/b - binding proteins of the green algae and higher plants; (3) chromophyte's LHC is supposed to be evolved from red algae LHC.
基金This study was supported by the grants from the National Natural Science Foundation of China （No. 30570080 and No. 30872153）, the Natural Science Foundation of Beijing （No. 7052020） and the Special Grant for Outstanding Young Scientist from Beijing Municipal Government （No. 2004B34）.
文摘Background Human metapneumovirus （hMPV） was discovered by scientists in the Netherlands as a novel respiratory virus in 2001 and had been found in children with acute respiratory tract infections （ARTI） in China. The objective of this study was to determine the importance of hMPV infection in children in Beijing and the genotypes of the circulating virus by the surveillance during a four-consecutive-year period.
文摘A54-year-old male smoker was admitted to our .hospital after undergoing the drainage of a leftperitonsillar abscess. Ten days prior to admission （day 1）, he complained of a ＂scratchy＂ throat with a fever of 38.5℃. He had an enlarged left tonsil with prominent exudate; white blood cell count （WCC） 8.8×109/L; platelet count （Plt） 103 × 109/L; chest X-ray was normal in a medical clinic. Then the patient received an intravenous injection of 4 million units of penicillin G every 6 hours and 500 mg of oral azithromycin once daily. But his condition worsened with a temperature of 41℃ and laryngoscopy confirmed a marked left-sided peritonsillar abscess which was drained. On day 10, the patient was transferred to our hospital after developing persistent fever, rigors, left-sided neck pain, pain on swallowing and purulent bloody sputum.
