Phenotypic and Genotypic Comparison of Vibrio in Seawater Fish from Batam and Mataram, Indonesia

Grouper and snapper are the potential fishery commodity in Indonesia with a high economic value, as well as an export commodity. A common disease in grouper and snapper aquaculture is vibriosis. Vibriosis is a disease caused by bacteria of the genus Vibrio. The aim of study was to compare between phenotypic and genotypic identification of Vibrio isolated from Batam and Mataram, Indonesia. Bacteria were isolated from anterior kidney and eye of fish, then grown in thiosulfate-citrate-bile salts-sucrose （TCBS） and incubated in room temperature （25-28 ~C） for 24 h, and identified using morphology and biochemical test. Bacterial isolates were extracted, amplified and sequenced on 16S rRNA region. Phylogenetic tree of bacteria was constructed using neighbor-joining and maximum-parsimony methods. The phenotypic identification was found six isolates of Vibrio from Batam, such as K alginolyticus, V. carchariae, K damselae, V. fluvialis, V. furnissii and K parahaemolyticus. Three isolates were found from Mataram, such as 1I. alginolyticus, V. carchariae and V. fluvialis. Blast analysis showed isolates of V. alginolyticus_btm and V. carchariae_btm homolog to V. parahaemolyticus strain DAHMV3; isolates of V. damselae_btm and K alginolyticus_mtr homolog to V. neocaledonicus strain MS1; isolates of V. parahaemolyticus__btm and V. furnisii_btm homolog with Photobacterium damselae subsp, damselae strain： 04Ya311 and isolate of K fluvialis_mtr homolog to V. azureus strain MMRF532, respectively. All phenotypic identification was not supported by molecular identification on 16S rRNA region. It was suggested that phenotypic identification should be supported by molecular examination, especially in identification of Vibrio species.

The Use of Seaweeds Aquaculture for Carbon Sequestration： A Strategy for Climate Change Mitigation

Seaweed has the ability to use carbon from the environment through photosynthesis to produce biomass. The aim of this study is to estimate carbon sequestration by seaweed aquaculture as a strategy for climate change mitigation. The study was undertaken at Gerupuk Bay, Lombok Island, West Nusa Tenggara Province, Indonesia. Four seaweed variants, such as Kappaphycus alvarezii var. Tambalang and Maumere, K. striatum and Eucheuma denticulatum, were cultivated with long-line system for three cultivation periods, starting from July to November, 2013. Each cultivation period was taken about 45 days. Parameters including weight increasement and carbon content of seaweeds were measured every 15 days of culture for each cultivation period in order to calculate carbon sequestration rate. The results showed that E. denticulatum had the highest carbon sequestration rate and significantly different （P 〈 0.05） compared with other variants for every cultivation period. Different seaweed variants have different capacity on carbon sequestration. Optimal utilization of the potential area for seaweed aquaculture could reduce a great quantity of CO2 from the atmosphere and help to mitigate global climate change process.