One of the important questions in space biology is the mechanisms underlying plant responses to an outer space environment,i.e.,how gene expression is altered in space.In this study,the transcriptome of Arabidopsis th...One of the important questions in space biology is the mechanisms underlying plant responses to an outer space environment,i.e.,how gene expression is altered in space.In this study,the transcriptome of Arabidopsis thaliana seedlings was analyzed as a part of Germany SIMBOX(science in microgravity box)spaceflight experiment on Shenzhou 8 spacecraft.This experiment involved the following treatments:spaceflight with microgravity(Fμg),spaceflight with 1g centrifugal force(F 1g),and ground 1g control(G 1g).Gene chips were used to screen gene expression differences in Arabidopsis thaliana seedlings among these treatments.Microarray analysis revealed that 621 genes were differentially expressed in samples Fμg vs.G 1g,249 genes in samples F 1g vs.G 1g,and 368 genes in samples Fμg vs.F 1g.Gene ontology analysis indicated that the genes were involved in metabolism of stress response,gravitropic response,and DNA damage and repair,suggesting that plants adjust these metabolic pathways to space environmental stress,microgravity,and radiation.展开更多
In cold surroundings, in both plants and animals (the class of fish, amphibian, mammal, etc.) tissue cells may appear many special anti-freeze proteins or cold adaptation-associated proteins for existence and developm...In cold surroundings, in both plants and animals (the class of fish, amphibian, mammal, etc.) tissue cells may appear many special anti-freeze proteins or cold adaptation-associated proteins for existence and development and regulate relevant gene expression at transcription and translation levels. With respect to mammals, the regulation and integration展开更多
基金supported by the National Key Basic Research Program (Grant No. 2011CB710902)China Manned Space Engineering ProgramInternational S&T Cooperation Program of China
文摘One of the important questions in space biology is the mechanisms underlying plant responses to an outer space environment,i.e.,how gene expression is altered in space.In this study,the transcriptome of Arabidopsis thaliana seedlings was analyzed as a part of Germany SIMBOX(science in microgravity box)spaceflight experiment on Shenzhou 8 spacecraft.This experiment involved the following treatments:spaceflight with microgravity(Fμg),spaceflight with 1g centrifugal force(F 1g),and ground 1g control(G 1g).Gene chips were used to screen gene expression differences in Arabidopsis thaliana seedlings among these treatments.Microarray analysis revealed that 621 genes were differentially expressed in samples Fμg vs.G 1g,249 genes in samples F 1g vs.G 1g,and 368 genes in samples Fμg vs.F 1g.Gene ontology analysis indicated that the genes were involved in metabolism of stress response,gravitropic response,and DNA damage and repair,suggesting that plants adjust these metabolic pathways to space environmental stress,microgravity,and radiation.
文摘In cold surroundings, in both plants and animals (the class of fish, amphibian, mammal, etc.) tissue cells may appear many special anti-freeze proteins or cold adaptation-associated proteins for existence and development and regulate relevant gene expression at transcription and translation levels. With respect to mammals, the regulation and integration
