Membrane proteins are vital components of the cell membrane and play crucial roles in various cellular activities.Analysis of membrane proteins is of paramount importance for studying molecular events inside cells and...Membrane proteins are vital components of the cell membrane and play crucial roles in various cellular activities.Analysis of membrane proteins is of paramount importance for studying molecular events inside cells and organisms and holds promising prospects for early disease diagnosis and treatment assessment.Benefiting from obvious merits including high affinity,high specificity and ease of modification,aptamers have been regarded as ideal molecular recognition elements in membrane protein analysis and molecular diagnostics strategies.This review summarised recent advances in membrane protein-specific aptamer screening,aptamer-based static and dynamic membrane protein analysis,and aptamer-based molecular diagnostic techniques.Prospects and challenges were also discussed.展开更多
Molecular imaging is a non-invasive method to image and analyze the concentration and activity of functional biomolecules in cells or in vivo at molecular level,and plays an increasing role in deep understanding of bi...Molecular imaging is a non-invasive method to image and analyze the concentration and activity of functional biomolecules in cells or in vivo at molecular level,and plays an increasing role in deep understanding of biological processes,early and accurate diagnosis of diseases,and evaluation of treatment.Nowadays,numerous novel molecular imaging probes have been developed,involving every biomedical imaging modality,such as optical imaging,photoacoustic imaging,magnetic resonance imaging,single-photon-emission computed tomography,and positron emission tomography.In this review,we summarize the development of current state-of-the-art molecular imaging probes.We introduce the design strategies of molecular probes and detailed imaging modalities,and highlight the properties of probes and biomedical imaging applications in cells and in vivo,including disease diagnosis,drug tracking,and imaging-guided surgery.Then we discuss the perspectives and challenges in this emerging field.We expect this review could inspire more effective molecular imaging probes to be developed,achieving the goal towards clinical practices.展开更多
Plasmonic metal nanomaterials with intrinsic surface–enhanced Raman scattering(SERS)and photothermal properties,especially AuAg nanoalloys with both the outstanding merits of Au and Ag nanocrystals,show huge applicat...Plasmonic metal nanomaterials with intrinsic surface–enhanced Raman scattering(SERS)and photothermal properties,especially AuAg nanoalloys with both the outstanding merits of Au and Ag nanocrystals,show huge application prospects in bacterial theranostics.However,the direct exposure of AuAg nanoalloys in external conditions probably cause undesirable reactions and poisonous metal ion leakage during SERS detection and photothermal antibacterial therapy process,which severely hinder bacterial theranostics applications.Herein,we report an ultrastable graphene–isolated AuAg nanoalloy(GAA)with AuAg core confined in few–layer graphitic shell as a versatile platform for bacterial detection and therapy.The encapsulation of graphene ensures the good stability of AuAg core,that its superior SERS and photothermal properties are therefore further guaranteed.GAA is used for SERS detection of two vital bacterial biomarkers(including corrosive cyanide and pyocyanin),exhibiting good SERS quantitative and multiplexing ability.GAA is further used for photothermal antibacterial therapy application,and ultrahigh antibacterial efficacies for both Gram–negative Escherichia coli and Gram–positive Staphylococcus aureus are achieved under 808 nm laser irradiation.This work proposes a valuable method to develop robust bacterial theranostic platform.展开更多
Investigation of metal–organic frameworks(MOFs)for biomedical applications has attracted much attention in recent years.MOFs are regarded as a promising class of nanocarriers for drug delivery owing to well-defined s...Investigation of metal–organic frameworks(MOFs)for biomedical applications has attracted much attention in recent years.MOFs are regarded as a promising class of nanocarriers for drug delivery owing to well-defined structure,ultrahigh surface area and porosity,tunable pore size,and easy chemical functionalization.In this review,the unique properties of MOFs and their advantages as nanocarriers for drug delivery in biomedical applications were discussed in the first section.Then,state-ofthe-art strategies to functionalize MOFs with therapeutic agents were summarized,including surface adsorption,pore encapsulation,covalent binding,and functional molecules as building blocks.In the third section,the most recent biological applications of MOFs for intracellular delivery of drugs,proteins,and nucleic acids,especially aptamers,were presented.Finally,challenges and prospects were comprehensively discussed to provide context for future development of MOFs as efficient drug delivery systems.展开更多
Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,...Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,the human skeletal muscle-specific ssDNA aptamers(HSM01,etc.)were selected and identified with Systematic Evolution of Ligands by Exponential Enrichment(SELEX).The HSM01 ssDNA aptamer preferentially interacted with human skeletal muscle cells in vitro.The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells.Furthermore,the ability of HSM01 ssDNA aptamer to target skeletal muscle cells was not affected by the formation of a disulfide bond with nanoliposomes in vitro or in vivo,suggesting a potential new approach for targeted drug delivery to skeletal muscles via liposomes.Therefore,this newly identified ssDNA aptamer and nanoliposome modification could be used for the treatment of human skeletal muscle diseases.展开更多
The interaction between biomolecules with their target ligands plays a great role in regulating biological functions.Aptamers are short oligonucleotide sequences that can specifically recognize target biomolecules via...The interaction between biomolecules with their target ligands plays a great role in regulating biological functions.Aptamers are short oligonucleotide sequences that can specifically recognize target biomolecules via structural complementarity and thus regulate related biological functions.In the past ten years,aptamers have made great progress in target biomolecule recognition,becoming a powerful tool to regulate biological functions.At present,there are many reviews on aptamers applied in biomolecular recognition,but few reviews pay attention to aptamer-based regulation of biological functions.Here,we summarize the approaches to enhancing aptamer affinity and the advancements of aptamers in regulating enzymatic activity,cellular immunity and cellular behaviors.Furthermore,this review discusses the challenges and future perspectives of aptamers in target recognition and biological functions regulation,aiming to provide some promising ideas for future regulation of biomolecular functions in a complex biological environment.展开更多
Molecular engineering of aptamers can confer exogenous biomedical properties that may be beneficial for various applications.In this study,a tumor-homing peptide modification strategy was developed to considerably enh...Molecular engineering of aptamers can confer exogenous biomedical properties that may be beneficial for various applications.In this study,a tumor-homing peptide modification strategy was developed to considerably enhance the accumulation and penetration abilities of the Sgc8c aptamer.Notably,the S2PM conjugate induced a much higher level of morphological variation in three-dimensional tumor microspheres(HCT116 cells)than in control groups,highlighting the importance of the homing and penetrating abilities derived from peptide.展开更多
Coronavirus disease 2019(COVID-19)is caused by a novel strain of coronavirus,designated as severe acute respiratory syndrome coronavirus 2(SARSCoV-2).It has caused a global pandemic rapidly sweeping across all countri...Coronavirus disease 2019(COVID-19)is caused by a novel strain of coronavirus,designated as severe acute respiratory syndrome coronavirus 2(SARSCoV-2).It has caused a global pandemic rapidly sweeping across all countries,bringing social and economic hardship to millions.Most countries have implemented early warning measures to detect,isolate,and treat patients infected with SARS-CoV-2.This minireview summarizes some of those steps,in particular,testing methods and drug development in the context of chemical biology,and discusses the molecular basis of COVID-19’s virulent transmissibility.展开更多
As a carrier of genetic information,DNA is a versatile module for fabricating nanostructures and nanodevices.Functional molecules could be integrated into DNA by precise base complementary pairing,greatly expanding th...As a carrier of genetic information,DNA is a versatile module for fabricating nanostructures and nanodevices.Functional molecules could be integrated into DNA by precise base complementary pairing,greatly expanding the functions of DNA nanomaterials.These functions endow DNA nanomaterials with great potential in the application of biomedical field.In recent years,functional DNA nanomaterials have been rapidly investigated and perfected.There have been reviews that classified DNA nanomaterials from the perspective of functions,while this review primarily focuses on the preparation methods of functional DNA nanomaterials.This review comprehensively introduces the preparation methods of DNA nanomaterials with functions such as molecular recognition,nanozyme catalysis,drug delivery,and biomedical material templates.Then,the latest application progress of functional DNA nanomaterials is systematically reviewed.Finally,current challenges and future prospects for functional DNA nanomaterials are discussed.展开更多
With the advantages of high sensitivity,rapid response,label-free,and simple operation,field effect transistor biosensors have shown promising application prospects in large-scale pathogen screening.However,in practic...With the advantages of high sensitivity,rapid response,label-free,and simple operation,field effect transistor biosensors have shown promising application prospects in large-scale pathogen screening.However,in practical biological fluids with relatively high ionic strength,such as saliva and serum,the Debye screening effect will weaken the interaction between FET biosensors and target bio-molecules,thereby affecting the sensing sensitivity and accuracy.展开更多
Surface-enhanced Raman spectroscopy(SERS)-based bioanalytical technique involves the interaction of SERS-active substrate with complex environment,which has aroused intensive research interests.Compared to the commonl...Surface-enhanced Raman spectroscopy(SERS)-based bioanalytical technique involves the interaction of SERS-active substrate with complex environment,which has aroused intensive research interests.Compared to the commonly used Au SERS substrates,Ag nanocrystals have larger optical absorption cross section and acceptable price,but they possess poor oxidation resistance and potential biotoxicity,and the occurrence of unnecessary chemical reactions is inevitable due to the direct contact with probe molecules.Herein,we report a graphene-isolated AuAg nanocrystal(GIAAN)with the SERS-active AuAg core confined in a nanospace of few-layer graphene shell,which possesses unique Raman peaks,high SERS activity,excellent stability,superior fluorescence quenching performance and good biocompatibility.Based on the limited solubility of GIAAN in water and organic solvents,it is able to spontaneously generate interfacial self-assembled GIAAN(ISA-GIAAN)film at immiscible two-phase interfaces without any inducer,and multiphase Raman analysis of both water-and lipid-soluble drug model molecules is further achieved.Moreover,the GIAAN is further non-covalently functionalized with polyoxyethylenestearyl ether(C18-PEG)to acquire GIAAN@PEG with good water-solubility for SERS quantitative analysis in homogeneous system and multimodal Raman imaging of MCF-7 cells.We expect the versatile GIAAN holds great potential to monitor drug metabolism and guide intended drug delivery in clinic trials.展开更多
Cells are the basic structural and functional units of organisms.Dynamic analysis and manipulation of specific components in living cells would provide valuable information for the study of related biological processe...Cells are the basic structural and functional units of organisms.Dynamic analysis and manipulation of specific components in living cells would provide valuable information for the study of related biological processes.Advances in fluorescence microscopy have allowed real-time monitoring of biological events at the molecular level.Meanwhile,the development of highperformance fluorescence probes has become a critical issue.Functional nucleic acids(FNAs)are oligonucleotides with special chemical and biological functions,and aptamers with excellent molecular recognition capability are one of the most important representatives.They have attracted extensive attention in the field of live-cell study,owing to intrinsic advantages of simple synthesis,convenient modification,low immunogenicity and high programmability.This review focuses on recent research progress in fluorescence imaging and manipulation of cells using FNAs,particularly aptamers,as the molecular tools.Finally,a summary is provided and the related challenges are discussed.展开更多
Complex biological environments and multiple physiological barriers significantly impede efficient accumulation and penetration of nanomaterials within tumor tissue for therapy.In situ energy conversion of nanomotors ...Complex biological environments and multiple physiological barriers significantly impede efficient accumulation and penetration of nanomaterials within tumor tissue for therapy.In situ energy conversion of nanomotors features autonomous movements and improves cancer treatment.However,one of the key challenges is to prepare nanomotors with an adequately small size,good biocompatibility,and precise positioning.Herein,we demonstrate a simple,ultrasmall,versatile,and real-time motion guidance strategy for magnetocatalytic CoPt@graphene navigators(MCGNs)that can enable highly efficient propulsion in the presence of H_(2)O_(2) or magnetic actuation.MCGNs act as highly diffusive delivery vehicles to promote tumor tissue targeting,and the amount of drug in the tumor was three times than without navigation.By engaging movements powered through in situ energy conversion,MCGNs gain considerable propulsion to penetrate a cell’s membrane and enhance intracellular delivery.展开更多
Specific regulation of the senescence-associated secretory phenotype(SASP)is vital to block senescence-induced detrimental cellular plasticity.Recently,some chemical compounds called senomorphics have demonstrated suc...Specific regulation of the senescence-associated secretory phenotype(SASP)is vital to block senescence-induced detrimental cellular plasticity.Recently,some chemical compounds called senomorphics have demonstrated such potential,but it remains challenging to achieve site-specific activation and real-time monitoring of the action of senomorphics,posing great obstacles for transformable applications.Here,we report a tailor-made hydrogen sulfide(H_(2)S)donor(Lyso-FH_(2)S-Gal)as a new class of molecule senomorphics for spatially controlled delivery of H_(2)S for visualization of regulation of cellular senescence.It comprises four functional moieties in a single molecular structure,including a lysosome-targeting group for cell recognition,a lysosomal enzyme-cleaved scaffold for site-specific activation,thiocarbamate as the H_(2)S precursor,and a switchable fluorophore for concurrent selfreporting of H_(2)S release and senescence imaging.Lyso-FH_(2)S-Gal exhibited remarkable response selectivity,sustained H_(2)S release,and 141-fold fluorescence enhancement.In cellular models,Lyso-FH_(2) S-Gal preferentially enriched in senescent cells over nonsenescent cells,and alleviated the levels of SASP and reactive oxygen species(ROS)in senescent cells,while remaining inert in nonsenescent cells.More impressively,it efficiently inhibited the SASPmediated crosstalk between senescent cells and surrounding nonsenescent cells,thereby preventing senescence propagation.This work offers a useful molecular tool with the hope for controlled intervention of senescence-related important biological processes.展开更多
Signal pathways participate in vital biological processes and regulate complex life activities through protein modifications.Protein modifications in signal pathways are accompanied by electron transfer.The study of e...Signal pathways participate in vital biological processes and regulate complex life activities through protein modifications.Protein modifications in signal pathways are accompanied by electron transfer.The study of electronic behavior helps to explore the physical and chemical processes in signal pathways,receiving extensive attention.There are some excellent reviews that have summarized methods for signal pathway detection,while few discussions are from an electron transfer perspective.This review describes the relationship between signal pathways and electron transfer in protein modification.Subsequently,we summarize the electron transfer-based detection methods,such as electrochemical,photoelectrochemical and electrochemiluminescence methods.Additionally,the applications of signal pathway detection in mechanism study and imaging are also reviewed.Finally,a comprehensive discussion of the summary and outlooks in this field is presented,aiming to provide valuable guidance for the molecular mechanism of life processes and the development of new analytical techniques.展开更多
Aptamers are single-stranded DNA or RNA sequences that can specifically bind with the target protein or molecule via specific secondary structures.Compared to antibody-drug conjugates(ADC),aptamer-drug conjugate(ApDC)...Aptamers are single-stranded DNA or RNA sequences that can specifically bind with the target protein or molecule via specific secondary structures.Compared to antibody-drug conjugates(ADC),aptamer-drug conjugate(ApDC)is also an efficient,targeted drug for cancer therapy with a smaller size,higher chemical stability,lower immunogenicity,faster tissue penetration,and facile engineering.Despite all these advantages,several key factors have delayed the clinical translation of ApDC,such as in vivo off-target effects and potential safety issues.In this review,we highlight the most recent progress in the development of ApDC and discuss solutions to the problems noted above.展开更多
Monitoring microbial metabolism is vital for revealing the mechanism of disease related to microbial metabolism and providing guidance for biomanufacturing processes optimization.However,it remains a grand challenge t...Monitoring microbial metabolism is vital for revealing the mechanism of disease related to microbial metabolism and providing guidance for biomanufacturing processes optimization.However,it remains a grand challenge to offer real-time insights into microbial metabolism owing to the complex and dynamic process.In this paper,the recent advances and prospects of optical biosensors including the organic,genetic coding and inorganic optical biosensors are briefly described for real-time monitoring of dynamic microbial metabolism.This paper points out that challenges remain in microbial heterogeneity.We believe that this work will inspire the application of developing new methods for single cell real-time analysis.展开更多
The rapid crystal growth of metal halide perovskite(MHP)nanocrystals inevitably leads to the generation of abundant crystal defects in the lattice.Here,defects-mediated long-lived charges and accompanying room-tempera...The rapid crystal growth of metal halide perovskite(MHP)nanocrystals inevitably leads to the generation of abundant crystal defects in the lattice.Here,defects-mediated long-lived charges and accompanying room-temperature persistent luminescence are demonstrated to be a general phenomenon in MHP nanocrystals.Density functional theory calculations suggest that the collaboration of Schottky and point defects enables upward cascading depletion for electron transfer in MHP nanocrystals,leading to the generation of long-lived photoexcited charges with lifetimes over 30 min.The excellent optical properties including the presence of long-lived charges,high charge separation efficiency,and broad absorption in the visible region make MHPs ideal candidates for both photocatalysis and photobiocatalysis.The MHPs were further integrated with enzymes to construct a light-driven biosynthetic system for the selective production of fine chemicals from CO_(2)with solar energy.The biosynthetic system can produce formate with a quantum yield of 3.24%,much higher than that of plants(∼0.2-1.6%).These findings will benefit the understanding of the optoelectronic properties of MHPs and further provide opportunities for the development of biosynthetic systems for solar-to-chemical synthesis.展开更多
Persistent luminescence nanoparticles(PLNPs)are a kind of phosphors that can remain luminescent for seconds to several days after the stoppage of excitation.Lanthanides show the special capability to largely broaden t...Persistent luminescence nanoparticles(PLNPs)are a kind of phosphors that can remain luminescent for seconds to several days after the stoppage of excitation.Lanthanides show the special capability to largely broaden the emission range and enhance the luminescence intensity of PLNPs due to their dense energy structure and unique electronic configurations.In the past decades,various methods have been developed for the synthesis of lanthanide-based PLNPs with excellent pe rsistent luminescence propertie s,and the lanthanide-based PLNPs are widely studied in areas including biome dicine,energy,and information storage.In this review,we summarized the research progress in the synthe sis of lanthanidebased PLNPs and outline d several typical synthesis methods.We discussed the fundamental concepts of preparation methods as well as the advantages and drawbacks of the typical synthetic approache s.Moreove r,the current challenges and the potential solutions for the development of lanthanide-based PLNP s are also discussed in an attempt to provide strate gies to further improve the optical properties of lanthanide-based PLNPs.We hope this review can contribute to the design of lanthanide-based PLNPs with desired properties and further promote their applications in biomedicine,energy,and information science.展开更多
The novel graphitic nanomaterial of metal graphitic nanocapsules(MGNs) with superior stability, unique optical properties and biocompatibility possess great potential in biomedical and bioanalytical applications. The ...The novel graphitic nanomaterial of metal graphitic nanocapsules(MGNs) with superior stability, unique optical properties and biocompatibility possess great potential in biomedical and bioanalytical applications. The graphitic shell can quench the background fluorescence interference from external environments via a fluorescence resonance energy transfer(FRET) process and even avoid unnecessary reactions catalyzed by inner metal core. The graphitic shell with several characteristic Raman bands itself can act as Raman signal probe or internal standard(IS), especially the 2D-band within the cellular Raman-silent region helps to reduce the interference signals from external conditions. The present context attempts to give a comprehensive overview about the preparation and unique properties of MGNs as well as their applications in SERS biodetection and bioimaging.展开更多
基金supported by the National Key Research and Development Project,China(No.2020YFA0909000)the National Natural Science Foundation of China(No.22107027)+1 种基金the Natural Science Foundation of Hunan Province,China(No.2023JJ20003)the Scientific Research Program of Furong Laboratory,China(No.2023SK2088).
文摘Membrane proteins are vital components of the cell membrane and play crucial roles in various cellular activities.Analysis of membrane proteins is of paramount importance for studying molecular events inside cells and organisms and holds promising prospects for early disease diagnosis and treatment assessment.Benefiting from obvious merits including high affinity,high specificity and ease of modification,aptamers have been regarded as ideal molecular recognition elements in membrane protein analysis and molecular diagnostics strategies.This review summarised recent advances in membrane protein-specific aptamer screening,aptamer-based static and dynamic membrane protein analysis,and aptamer-based molecular diagnostic techniques.Prospects and challenges were also discussed.
基金supported by the National Key R&D Program of China (2020YFA0210800, 2020YFA0909000)the National Natural Science Foundation of China (22176035, U21A20377, 21874024, 21890744, 22107027, 22074036)Shenzhen Science and Technology Program (RCBS20200714114821377)
文摘Molecular imaging is a non-invasive method to image and analyze the concentration and activity of functional biomolecules in cells or in vivo at molecular level,and plays an increasing role in deep understanding of biological processes,early and accurate diagnosis of diseases,and evaluation of treatment.Nowadays,numerous novel molecular imaging probes have been developed,involving every biomedical imaging modality,such as optical imaging,photoacoustic imaging,magnetic resonance imaging,single-photon-emission computed tomography,and positron emission tomography.In this review,we summarize the development of current state-of-the-art molecular imaging probes.We introduce the design strategies of molecular probes and detailed imaging modalities,and highlight the properties of probes and biomedical imaging applications in cells and in vivo,including disease diagnosis,drug tracking,and imaging-guided surgery.Then we discuss the perspectives and challenges in this emerging field.We expect this review could inspire more effective molecular imaging probes to be developed,achieving the goal towards clinical practices.
基金funding support from the National Key Research and Development Program of China(Nos.2022YFC2403501,2020YFA0210800)National Natural Science Foundation of China(No.22225401)+1 种基金Science and Technology Innovation Program of Hunan Province(No.2020RC4017)China Postdoctoral Science Foundation(No.2021M701145)。
文摘Plasmonic metal nanomaterials with intrinsic surface–enhanced Raman scattering(SERS)and photothermal properties,especially AuAg nanoalloys with both the outstanding merits of Au and Ag nanocrystals,show huge application prospects in bacterial theranostics.However,the direct exposure of AuAg nanoalloys in external conditions probably cause undesirable reactions and poisonous metal ion leakage during SERS detection and photothermal antibacterial therapy process,which severely hinder bacterial theranostics applications.Herein,we report an ultrastable graphene–isolated AuAg nanoalloy(GAA)with AuAg core confined in few–layer graphitic shell as a versatile platform for bacterial detection and therapy.The encapsulation of graphene ensures the good stability of AuAg core,that its superior SERS and photothermal properties are therefore further guaranteed.GAA is used for SERS detection of two vital bacterial biomarkers(including corrosive cyanide and pyocyanin),exhibiting good SERS quantitative and multiplexing ability.GAA is further used for photothermal antibacterial therapy application,and ultrahigh antibacterial efficacies for both Gram–negative Escherichia coli and Gram–positive Staphylococcus aureus are achieved under 808 nm laser irradiation.This work proposes a valuable method to develop robust bacterial theranostic platform.
基金supported by the National Natural Science Foundation of China(Grant No.21827811)Research and development plan of key areas in Hunan Province(Grant No.2019SK2201)Innovation science and technology plan of Hunan Province(Grant No.2017XK2103).
文摘Investigation of metal–organic frameworks(MOFs)for biomedical applications has attracted much attention in recent years.MOFs are regarded as a promising class of nanocarriers for drug delivery owing to well-defined structure,ultrahigh surface area and porosity,tunable pore size,and easy chemical functionalization.In this review,the unique properties of MOFs and their advantages as nanocarriers for drug delivery in biomedical applications were discussed in the first section.Then,state-ofthe-art strategies to functionalize MOFs with therapeutic agents were summarized,including surface adsorption,pore encapsulation,covalent binding,and functional molecules as building blocks.In the third section,the most recent biological applications of MOFs for intracellular delivery of drugs,proteins,and nucleic acids,especially aptamers,were presented.Finally,challenges and prospects were comprehensively discussed to provide context for future development of MOFs as efficient drug delivery systems.
基金supported by the National Key Research and Development Plan(2018YFC2001500)National Natural Science Foundation of China(81972254,82172098).
文摘Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,the human skeletal muscle-specific ssDNA aptamers(HSM01,etc.)were selected and identified with Systematic Evolution of Ligands by Exponential Enrichment(SELEX).The HSM01 ssDNA aptamer preferentially interacted with human skeletal muscle cells in vitro.The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells.Furthermore,the ability of HSM01 ssDNA aptamer to target skeletal muscle cells was not affected by the formation of a disulfide bond with nanoliposomes in vitro or in vivo,suggesting a potential new approach for targeted drug delivery to skeletal muscles via liposomes.Therefore,this newly identified ssDNA aptamer and nanoliposome modification could be used for the treatment of human skeletal muscle diseases.
基金supported by the National Natural Science Foundation of China(Nos.22174038,21925401,21904037).
文摘The interaction between biomolecules with their target ligands plays a great role in regulating biological functions.Aptamers are short oligonucleotide sequences that can specifically recognize target biomolecules via structural complementarity and thus regulate related biological functions.In the past ten years,aptamers have made great progress in target biomolecule recognition,becoming a powerful tool to regulate biological functions.At present,there are many reviews on aptamers applied in biomolecular recognition,but few reviews pay attention to aptamer-based regulation of biological functions.Here,we summarize the approaches to enhancing aptamer affinity and the advancements of aptamers in regulating enzymatic activity,cellular immunity and cellular behaviors.Furthermore,this review discusses the challenges and future perspectives of aptamers in target recognition and biological functions regulation,aiming to provide some promising ideas for future regulation of biomolecular functions in a complex biological environment.
基金supported by the National Key R&D Program of China(no.2018YFA0902300)the Huxiang Young Talent Program from Hunan Province(no.2019RS2022)+1 种基金the National Natural Science Foundation of China(no.91959102)Postgraduate Research and Innovation Project from Hunan Province(no.CX20190269).
文摘Molecular engineering of aptamers can confer exogenous biomedical properties that may be beneficial for various applications.In this study,a tumor-homing peptide modification strategy was developed to considerably enhance the accumulation and penetration abilities of the Sgc8c aptamer.Notably,the S2PM conjugate induced a much higher level of morphological variation in three-dimensional tumor microspheres(HCT116 cells)than in control groups,highlighting the importance of the homing and penetrating abilities derived from peptide.
基金This research was made possible as a result of a generous grant from the National Key Research Program(no.2020YFC0842800)the Science and Technology Project of Hunan Province(no.2020SK3008)the Ministry of Science and Technology of China(nos.2016YFA0201600 and 2018YFE0117200).
文摘Coronavirus disease 2019(COVID-19)is caused by a novel strain of coronavirus,designated as severe acute respiratory syndrome coronavirus 2(SARSCoV-2).It has caused a global pandemic rapidly sweeping across all countries,bringing social and economic hardship to millions.Most countries have implemented early warning measures to detect,isolate,and treat patients infected with SARS-CoV-2.This minireview summarizes some of those steps,in particular,testing methods and drug development in the context of chemical biology,and discusses the molecular basis of COVID-19’s virulent transmissibility.
基金supported by the National Natural Science Foundation of China(Nos.21925401,22174038,and 21904037)the Natural Science Foundation of Hunan Province(Nos.2022JJ20005 and 2020JJ4173).
文摘As a carrier of genetic information,DNA is a versatile module for fabricating nanostructures and nanodevices.Functional molecules could be integrated into DNA by precise base complementary pairing,greatly expanding the functions of DNA nanomaterials.These functions endow DNA nanomaterials with great potential in the application of biomedical field.In recent years,functional DNA nanomaterials have been rapidly investigated and perfected.There have been reviews that classified DNA nanomaterials from the perspective of functions,while this review primarily focuses on the preparation methods of functional DNA nanomaterials.This review comprehensively introduces the preparation methods of DNA nanomaterials with functions such as molecular recognition,nanozyme catalysis,drug delivery,and biomedical material templates.Then,the latest application progress of functional DNA nanomaterials is systematically reviewed.Finally,current challenges and future prospects for functional DNA nanomaterials are discussed.
基金The National Key R&D Program of China(2017YFA0208000,2021YFA1202400)the New Cornerstone Science Foundation through the XPLORER PRIZE are acknowledged for research fundingthe Core Facility of Wuhan University for ATR-FTIR,SEM,and XPS analysis.The study was approved by the Ethics Committee of Renmin Hospital of Wuhan University(WDRY2022-K257)。
文摘With the advantages of high sensitivity,rapid response,label-free,and simple operation,field effect transistor biosensors have shown promising application prospects in large-scale pathogen screening.However,in practical biological fluids with relatively high ionic strength,such as saliva and serum,the Debye screening effect will weaken the interaction between FET biosensors and target bio-molecules,thereby affecting the sensing sensitivity and accuracy.
基金the Hunan Provincial Natural Science Foundation of China(Nos.2020RC4017,2018JJ1007)the Science and Technology Development Fund,Macao(No.196/2017/A3).
文摘Surface-enhanced Raman spectroscopy(SERS)-based bioanalytical technique involves the interaction of SERS-active substrate with complex environment,which has aroused intensive research interests.Compared to the commonly used Au SERS substrates,Ag nanocrystals have larger optical absorption cross section and acceptable price,but they possess poor oxidation resistance and potential biotoxicity,and the occurrence of unnecessary chemical reactions is inevitable due to the direct contact with probe molecules.Herein,we report a graphene-isolated AuAg nanocrystal(GIAAN)with the SERS-active AuAg core confined in a nanospace of few-layer graphene shell,which possesses unique Raman peaks,high SERS activity,excellent stability,superior fluorescence quenching performance and good biocompatibility.Based on the limited solubility of GIAAN in water and organic solvents,it is able to spontaneously generate interfacial self-assembled GIAAN(ISA-GIAAN)film at immiscible two-phase interfaces without any inducer,and multiphase Raman analysis of both water-and lipid-soluble drug model molecules is further achieved.Moreover,the GIAAN is further non-covalently functionalized with polyoxyethylenestearyl ether(C18-PEG)to acquire GIAAN@PEG with good water-solubility for SERS quantitative analysis in homogeneous system and multimodal Raman imaging of MCF-7 cells.We expect the versatile GIAAN holds great potential to monitor drug metabolism and guide intended drug delivery in clinic trials.
基金supported by the National Key Research Program(2018YFC1602900,2019YFA0905800)the National Natural Science Foundation of China(NSFC 21922404)the Science and Technology Project of Hunan Province(2017XK2103,2018JJ3034,2019SK2201,2018RS3035,2020SK3008)。
文摘Cells are the basic structural and functional units of organisms.Dynamic analysis and manipulation of specific components in living cells would provide valuable information for the study of related biological processes.Advances in fluorescence microscopy have allowed real-time monitoring of biological events at the molecular level.Meanwhile,the development of highperformance fluorescence probes has become a critical issue.Functional nucleic acids(FNAs)are oligonucleotides with special chemical and biological functions,and aptamers with excellent molecular recognition capability are one of the most important representatives.They have attracted extensive attention in the field of live-cell study,owing to intrinsic advantages of simple synthesis,convenient modification,low immunogenicity and high programmability.This review focuses on recent research progress in fluorescence imaging and manipulation of cells using FNAs,particularly aptamers,as the molecular tools.Finally,a summary is provided and the related challenges are discussed.
基金financially supported by the National Natural Science Foundation of China(grant no.21522501)Hunan Provincial Natural Science Foundation of China(grant no.2020RC4017)+1 种基金National Postdoctoral Program for Innovative Talents of China(grant no.BX20190111)the Science and Technology Development Fund,Macao(no.196/2017/A3).
文摘Complex biological environments and multiple physiological barriers significantly impede efficient accumulation and penetration of nanomaterials within tumor tissue for therapy.In situ energy conversion of nanomotors features autonomous movements and improves cancer treatment.However,one of the key challenges is to prepare nanomotors with an adequately small size,good biocompatibility,and precise positioning.Herein,we demonstrate a simple,ultrasmall,versatile,and real-time motion guidance strategy for magnetocatalytic CoPt@graphene navigators(MCGNs)that can enable highly efficient propulsion in the presence of H_(2)O_(2) or magnetic actuation.MCGNs act as highly diffusive delivery vehicles to promote tumor tissue targeting,and the amount of drug in the tumor was three times than without navigation.By engaging movements powered through in situ energy conversion,MCGNs gain considerable propulsion to penetrate a cell’s membrane and enhance intracellular delivery.
基金supported by the National Natural Science Foundation of China(grant nos.NSFC22274044 and 21877031)the National Key Research and Development Program of China(grant no.2020YFA0210802)the Science and Technology Innovation Program of Hunan Province(grant no.2018RS3043).
文摘Specific regulation of the senescence-associated secretory phenotype(SASP)is vital to block senescence-induced detrimental cellular plasticity.Recently,some chemical compounds called senomorphics have demonstrated such potential,but it remains challenging to achieve site-specific activation and real-time monitoring of the action of senomorphics,posing great obstacles for transformable applications.Here,we report a tailor-made hydrogen sulfide(H_(2)S)donor(Lyso-FH_(2)S-Gal)as a new class of molecule senomorphics for spatially controlled delivery of H_(2)S for visualization of regulation of cellular senescence.It comprises four functional moieties in a single molecular structure,including a lysosome-targeting group for cell recognition,a lysosomal enzyme-cleaved scaffold for site-specific activation,thiocarbamate as the H_(2)S precursor,and a switchable fluorophore for concurrent selfreporting of H_(2)S release and senescence imaging.Lyso-FH_(2)S-Gal exhibited remarkable response selectivity,sustained H_(2)S release,and 141-fold fluorescence enhancement.In cellular models,Lyso-FH_(2) S-Gal preferentially enriched in senescent cells over nonsenescent cells,and alleviated the levels of SASP and reactive oxygen species(ROS)in senescent cells,while remaining inert in nonsenescent cells.More impressively,it efficiently inhibited the SASPmediated crosstalk between senescent cells and surrounding nonsenescent cells,thereby preventing senescence propagation.This work offers a useful molecular tool with the hope for controlled intervention of senescence-related important biological processes.
基金supported by the National Natural Science Foundation of China(22174038,21925401,21904037,52221001)the Natural Science Foundation of Hunan Province(2022JJ20005,2020JJ4173)。
文摘Signal pathways participate in vital biological processes and regulate complex life activities through protein modifications.Protein modifications in signal pathways are accompanied by electron transfer.The study of electronic behavior helps to explore the physical and chemical processes in signal pathways,receiving extensive attention.There are some excellent reviews that have summarized methods for signal pathway detection,while few discussions are from an electron transfer perspective.This review describes the relationship between signal pathways and electron transfer in protein modification.Subsequently,we summarize the electron transfer-based detection methods,such as electrochemical,photoelectrochemical and electrochemiluminescence methods.Additionally,the applications of signal pathway detection in mechanism study and imaging are also reviewed.Finally,a comprehensive discussion of the summary and outlooks in this field is presented,aiming to provide valuable guidance for the molecular mechanism of life processes and the development of new analytical techniques.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(No.Y21C050001,China)Zhejiang Provincial Research Center for Diagnosis and Treatment of Major Diseases(No.JBZX-202003,China)+2 种基金the National Natural Science Foundation of China(Nos.22104132 and 22204144)the Zhejiang Province“Kunpeng”Program。
文摘Aptamers are single-stranded DNA or RNA sequences that can specifically bind with the target protein or molecule via specific secondary structures.Compared to antibody-drug conjugates(ADC),aptamer-drug conjugate(ApDC)is also an efficient,targeted drug for cancer therapy with a smaller size,higher chemical stability,lower immunogenicity,faster tissue penetration,and facile engineering.Despite all these advantages,several key factors have delayed the clinical translation of ApDC,such as in vivo off-target effects and potential safety issues.In this review,we highlight the most recent progress in the development of ApDC and discuss solutions to the problems noted above.
基金The National Natural Science Foundation of China(21925401)is acknowledged for research funding。
文摘Monitoring microbial metabolism is vital for revealing the mechanism of disease related to microbial metabolism and providing guidance for biomanufacturing processes optimization.However,it remains a grand challenge to offer real-time insights into microbial metabolism owing to the complex and dynamic process.In this paper,the recent advances and prospects of optical biosensors including the organic,genetic coding and inorganic optical biosensors are briefly described for real-time monitoring of dynamic microbial metabolism.This paper points out that challenges remain in microbial heterogeneity.We believe that this work will inspire the application of developing new methods for single cell real-time analysis.
基金This work was supported by the National Natural Science Foundation of China(nos.21925401 and 21904100)the National Key R&D Program of China(no.2017YFA0208000).Q.Y.and Y.Y.thank the large-scale instrument and equipment-sharing foundation of Wuhan University。
文摘The rapid crystal growth of metal halide perovskite(MHP)nanocrystals inevitably leads to the generation of abundant crystal defects in the lattice.Here,defects-mediated long-lived charges and accompanying room-temperature persistent luminescence are demonstrated to be a general phenomenon in MHP nanocrystals.Density functional theory calculations suggest that the collaboration of Schottky and point defects enables upward cascading depletion for electron transfer in MHP nanocrystals,leading to the generation of long-lived photoexcited charges with lifetimes over 30 min.The excellent optical properties including the presence of long-lived charges,high charge separation efficiency,and broad absorption in the visible region make MHPs ideal candidates for both photocatalysis and photobiocatalysis.The MHPs were further integrated with enzymes to construct a light-driven biosynthetic system for the selective production of fine chemicals from CO_(2)with solar energy.The biosynthetic system can produce formate with a quantum yield of 3.24%,much higher than that of plants(∼0.2-1.6%).These findings will benefit the understanding of the optoelectronic properties of MHPs and further provide opportunities for the development of biosynthetic systems for solar-to-chemical synthesis.
基金Project supported by the National Key R&D Program of China(2021YFA1202400,2017YFA0208000)the National Natural Science Foundation of China(21925401,21904033,51902177)the Fundamental Research Funds for the Central Universities(2042021 kf0036)。
文摘Persistent luminescence nanoparticles(PLNPs)are a kind of phosphors that can remain luminescent for seconds to several days after the stoppage of excitation.Lanthanides show the special capability to largely broaden the emission range and enhance the luminescence intensity of PLNPs due to their dense energy structure and unique electronic configurations.In the past decades,various methods have been developed for the synthesis of lanthanide-based PLNPs with excellent pe rsistent luminescence propertie s,and the lanthanide-based PLNPs are widely studied in areas including biome dicine,energy,and information storage.In this review,we summarized the research progress in the synthe sis of lanthanidebased PLNPs and outline d several typical synthesis methods.We discussed the fundamental concepts of preparation methods as well as the advantages and drawbacks of the typical synthetic approache s.Moreove r,the current challenges and the potential solutions for the development of lanthanide-based PLNP s are also discussed in an attempt to provide strate gies to further improve the optical properties of lanthanide-based PLNPs.We hope this review can contribute to the design of lanthanide-based PLNPs with desired properties and further promote their applications in biomedicine,energy,and information science.
基金financially supported by the National Natural Science Foundation of China (No. 21522501)Hunan Provincial Natural Science Foundation of China (No. 2018JJ1007)Science and Technology Development Fund, Macao (No. 196/2017/A3)
文摘The novel graphitic nanomaterial of metal graphitic nanocapsules(MGNs) with superior stability, unique optical properties and biocompatibility possess great potential in biomedical and bioanalytical applications. The graphitic shell can quench the background fluorescence interference from external environments via a fluorescence resonance energy transfer(FRET) process and even avoid unnecessary reactions catalyzed by inner metal core. The graphitic shell with several characteristic Raman bands itself can act as Raman signal probe or internal standard(IS), especially the 2D-band within the cellular Raman-silent region helps to reduce the interference signals from external conditions. The present context attempts to give a comprehensive overview about the preparation and unique properties of MGNs as well as their applications in SERS biodetection and bioimaging.