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Cloning and enzymology analysis of farnesyl pyrophosphate synthase gene from a superior strain of Artemisia annua L. 被引量:6
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作者 ZHAOYujun YEHechun 《Chinese Science Bulletin》 SCIE EI CAS 2003年第1期63-67,共5页
A cDNA(af1) encoding farnesyl pyrophosphate synthase AaFPS1 (FPS, EC2.5.1.1/EC2.5.1.10) from a high yield Artemisia annua strain 025 has been cloned from its cDNA library. Sequence analysis showed that the cDNA en-cod... A cDNA(af1) encoding farnesyl pyrophosphate synthase AaFPS1 (FPS, EC2.5.1.1/EC2.5.1.10) from a high yield Artemisia annua strain 025 has been cloned from its cDNA library. Sequence analysis showed that the cDNA en-coded a protein of 343 amino acid (aa) residues with mo-lecular weight of 39 kD. Deduced aa sequence of the cDNA was similar to FPS from other plants, yeast and mammals, containing 5 conserved domains found in both prenyl trans-ferase and polyprenyl synthase. The expression of the cDNA in Escherichia coli showed measurable specific activity of FPS in vitro. The enzyme was purified by ion exchange chromatography and its kinetics was measured. These re-sults would further promote the molecular regulation of ar-temisinin biosynthesis. 展开更多
关键词 黄花蒿 法尼基焦磷酸合成酶 基因克隆 酶学分析 青蒿素
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