美国蒙特克莱尔州立大学图论教学特点研究及启示

图论是理工科专业学生开设的一门重要基础课程。该文研究美国蒙特克莱尔州立大学图论课程的教学方法,发现其在教学过程中融入大量应用实例,增强学生的学习主动性,完美达到理论联系实际的教学目标。分析其应用型教学特点,可以在国内图论课程的教学中,注重引入相关应用及研究理念,激发学生学习兴趣,明确图论课程的实际应用价值,对于图论课程的教学改革和课程建设具有一定的参考意义。

Microbial Degradation of Organic Contaminants in Streambed/Floodplain Sediments in Passaic River—New Jersey Area

This paper is intended to explore soil organic matter and carbon isotope fractionation at three locations of the Passaic River to determine if microbial degradation of organic contaminants in soil is correlated to the surrounding physical environment. Microbial degradation of organic contaminants is important for the detoxification of toxic substances thereby minimizing stagnation in the environment and accumulating in the food chain. Since organic contaminants are not easily dissolved in water, they will penetrate sediment and end up enriching the adjacent soil. The hypothesis that we are testing is microbial activity and carbon isotope fractionation will be greater in preserved soils than urban soils. The reason why this is expected to be the case is the expectation of higher microbial activity in preserved environments due to less exposure to pollutants, better soil structure, higher organic matter content, and more favorable conditions for microbial growth. This is contrasted with urban soils, which are impacted by pollutants and disturbances, potentially inhibiting microbial activity. We wish to collect soil samples adjacent to the Passaic River at a pristine location, Great Swamp Wildlife Refuge, a suburban location, Goffle Brook Park, Hawthorne NJ, and an urban location, Paterson NJ. These soil samples will be weighed for soil organic matter (SOM) and weighed for isotope ratio mass spectrometry (IRMS) to test organic carbon isotopes. High SOM and δ13C depletion activity indicate microbial growth based on the characteristics of the soil horizon rather than the location of the soil sample which results in degradation of organic compounds.

Black Garlic as an Antiviral for Herpes Simplex Virus-2 in Lung Cells

Allicin, an antioxidant, is known for providing garlic with its unique fragrance and taste, as well as for its antimicrobial properties. Black garlic, a fermented form of garlic, contains higher levels of antioxidants than fresh garlic. Antioxidants play a vital role in alleviating cellular stress during viral infections. Viral infections result in oxidative stress through the production of reactive oxidative species (ROS). A prolonged state of oxidative stress can result in cell death, DNA damage, and disease progression. In this study, black garlic extract (BGE) is evaluated for its ability to mitigate cytopathic effects and oxidative stress caused by herpes simplex virus-2 (HSV-2) infections in vitro. Antiviral assays were performed to determine the percent of viral inhibition resulting from treatment with the BGE. ROS-GloTM H2O2 assays were then completed to measure the post-infection ROS levels of BGE-treated virus and cells. The results thus far suggest that BGE may inhibit viral infection and decrease levels of oxidative stress.

HISP-1 Inhibits HSV-1 Infection in Cultured Vero Cells

Herpes simplex virus-1 (HSV-1) remains a leading cause of viral disease worldwide and is spread by direct contact with infected lesions. There is no vaccine against HSV-1 infections and there remains a need to identify therapeutics that could reduce the spread. In this study various hispolon compounds were analyzed to determine their antiviral potential against HSV-1 infections in cultured Vero cells. To determine the effects on infectivity and possible mechanisms of inhibition, the following assays were conducted. In vitro cytotoxicity assays were conducted to determine the effect of the compounds on cell viability and the maximum non-cytotoxic concentrations. Antiviral assays measured cell viability, percent inhibition of infection following treatment with the compounds, and the effect on the viral infection cycle. These effects were visualized using inverted light and fluorescent microscopy. Of the 24 hispolons tested, only hispolon pyrazole-1 (HISP-1) demonstrated antiviral effects. HISP-1 was demonstrated to effect early stages in HSV-1 infection in cultured Vero cells (attachment, penetration, and post-penetration). In silico modeling analyses were conducted to analyze the interactions between HISP-1 and viral glycoprotein D (gD). HISP-1 is safe at concentrations tested and is effective in inhibiting infection of HSV-1 in cultured cells. HISP-1 has potential for therapeutic use as an antiviral against HSV-1 infection, could work in synergy with other antivirals that work be a different modality, and could be developed as a component of a topical agent to reduce the spread of HSV-1 infections.

长江三角洲城市群城镇用地扩展的时空特征及驱动因素分析

选取长江三角洲城市群1995—2015年的城镇用地监测数据,采用城镇扩展强度指数、分形维度以及城镇化率3个指标分析其城镇用地时空演变特征,并基于GWR对其演变的驱动力进行分析。结果表明,长江三角洲城市群城镇用地扩展强度整体呈先上升后下降再缓慢上升的趋势,其中高速扩展型的城镇占总数的7.7%,中速扩展型城镇占总数的20.2%,低速扩展型城镇占总数的72.1%。1995—2015年长江三角洲城市群各省市的分形维数都有所增加,安徽省分形维数较低,变化幅度较大,江苏省较为平稳,上海市呈平稳上升趋势,且江苏省和上海市高于长江三角洲城市群的平均水平。1995—2015年,经济、医疗卫生、教育是长江三角洲城市群南部城市的主要驱动力,第一产业、交通运输和人口则是北部城市的主要驱动力。从空间维度视角来看,1995年经济和医疗卫生是浙江省和上海市的主要驱动力,随着时间的推移,人口是城市群北部的主要驱动力,医疗卫生是城市群南部的主要驱动力。

基于订正ALPHA差值谱的热红外温度与发射率分离算法

与可见光遥感不同,热红外传感器测得的辐射亮度值是温度和发射率的函数,因此,对于热红外遥感,温度和发射率的分离是一个关键问题?本研究从普朗克方程的维恩近似出发,定义并推导出与温度无关的ALPHA差值谱,并进一步提出消除维恩近似影响的修正项.以此为基础,在不考虑大气下行辐射影响的条件下,借鉴ALPHA导出发射率法和ASTERTES算法的优点,提出一个新的发射率温度分离算法.与现有发射率温度分离算法相比,该算法的优点是原理清楚,流程简单,求解速度快,结果精度高,并且适用的温度和下垫面范围宽.

GWLS-SVR模型的红枣树叶片叶绿素含量估算

叶绿素含量是红枣树光合作用能力、生长状况、营养状况的指示剂,不同地理位置种植的红枣树受到自然、人为等因素的影响,叶绿素含量分布有所不同,该研究实地测定了若羌县枣树叶片高光谱反射率及表征叶绿素含量的枣树叶片SPAD(soil plant analysis development)值。为了高效无损地估算红枣树叶片SPAD值,计算了红枣树叶片SPAD值全局莫兰指数,以SPAD值和高光谱波段之间的相关性为基础,通过C P统计量计算重要程度高的特征波段,运用地理加权最小二乘支持向量回归GWLS-SVR(geographically weighted least squares-support vector regression)模型对红枣树叶片SPAD值进行预测,与多元线性回归(MLR)、支持向量机回归(SVR)模型比较并探讨GWLS-SVR模型估算红枣树叶片SPAD值的能力。结果表明:(1)光谱一阶导数可以有效去除噪声并突出光谱信息尤其是492~510,542~543,642~652,657~670和682~692 nm区间内显著的提高了与SPAD值的相关性。(2)C P统计量方法能够有效的选择敏感区间的特征波段,进而提高模型估算精度,由统计量方法计算出原始光谱重要程度最高的两个变量为595与696 nm,光谱一阶导数的特征波段为688 nm。其中对于同一个敏感波段区间的波段组合总有单个波段的统计量低于多个波段组合的统计量,这可能是相近波段间的较强共线性导致的。(3)若羌县红枣树叶片SPAD值存在显著的空间聚集性,全局莫兰指数为0.1258(p<0.1),适合建立考虑空间位置的GWLS-SVR模型。(4)结合Bootstrap再抽样与t检验模型检验得到结合地理位置信息的GWLS-SVR模型总体上估算能力优于SVR和MLR模型,且结果高度显著(p<0.001),其中基于光谱一阶导数的GWLS-SVR模型为最优的红枣树叶片SPAD值估算模型(R 2为0.975,MSE为1.082),能够为高光谱定量反演红枣树SPAD值进而快速无损的监测红枣生长状况提供一定参考。

A comparative study of seismic provisions between International Building Code 2003 and Uniform Building Code 1997

This study focuses on the comparison of the Uniform Building Code （UBC） 1997 and International Building Code （IBC） 2003 in relation to the seismic design and analysis of special steel moment resisting frame buildings （SMRF）. This paper formulates a numerical study of a steel SMRF building, studied in four different situations, namely： as an office building in San Francisco; as an office building in Sacramento; as an essential facility in San Francisco, and as an essential facility in Sacramento. The analytical results of the model buildings are then compared and analyzed taking note of any significant differences. This case study explores variations in the results obtained using the two codes, particularly the design base shear and drift ratios as they relate to different locations and occupancy use. This study also proves that IBC 2003 is more stringent for the redundancy factor under design category E for the SMRF building, and drift limits for essential facilities.

Energy Balance-Based SWAT Model to Simulate the Mountain Snowmelt and Runoff——Taking the Application in Juntanghu Watershed(China) as an Example

In order to predict long-term flooding under extreme weather conditions in central Asia, an energy balance-based distributed snowmelt runoff model was developed and coupled with the Soil and Water Assessment Tool(SWAT) model. The model was tested at the Juntanghu watershed on the northern slope of the Tian Shan Mountains, Xinjiang,China. We compared the performances of temperature-index method and energy balanced method in SWAT model by taking Juntanghu river basin as an application example(as the simulation experiment was conducted in Juntanghu River, we call the energy balanced method as SWAT-JTH). The results suggest that the SWAT snowmelt model had overall Nash-Sutcliffe efficiency(NSE) coefficients ranging from 0.61 to 0.85 while the physical based approach had NSE coefficients ranging from 0.58 to0.69. Overall, on monthly scale, the SWAT model provides better results than that from the SWAT-JTH model. However, results generated from both methods seem to be fairly close at a daily scale. Thestructure of the temperature-index method is simple and produces reasonable simulation results if the parameters are well within empirical ranges. Although the data requirement for the energy balance method in current observation is difficult to meet and the existence of uncertainty is associated with the experimental approaches of physical processes, the SWAT-JTH model still produced a reasonably high NSE. We conclude that using temperature-index methods to simulate the snowmelt process is sufficient, but the energy balance-based model is still a good choice to simulate extreme weather conditions especially when the required data input for the model is acquired.

The synergistic effects of green tea polyphenols and antibiotics against potential pathogens

Green tea leaves contain many polyphenolic compounds such as (-)-epicatechin (EC), (-)-epicatechin-3-gallate (ECG), (-)-epigallocatechin (EGC), and (-)epigallocatechin-3-gallate (EGCG). These polyphenol compounds have been implicated to have distinct properties that combat the harmful effects of cell proliferation. They contain certain anti-viral and antibacterial properties that inhibit growth. In this study, 1% green tea and modified lipophilic green tea polyphenols (GTP and LTP) were used in combination with the most commonly prescribed antibiotics to study their effects on gram-positive, gram-negative, and acid-fast bacteria. The results indicated that 1% GTP and 1% LTP provided different synergistic effects on several antibiotics in various bacteria. It was found that 1% GTP works the best synergistically against Enterobacter aerogenes, making the resistant strain susceptible to 8 out of 12 antibiotics used. 1% LTP worked the best on Escherichia coli and was able to convert 7 antibiotic resistant categories to susceptible. In addition, 1% LTP was also able to inhibit the growth of Serratia marcescens synergistically with 3 antibiotics. These results suggest that 1% GTP and 1% LTP provide beneficial effects on selected antibiotics against microbial growth and are able to reverse the antibiotic resistance to susceptible. Green tea polyphenols could serve as natural alternatives to combat against antibiotic resistance pathogens.

Black carbon and polycyclic aromatic hydrocarbons (PAHs) in surface sediments of China’s marginal seas

This study investigates the distribution of black carbon (BC) and its correlation with total polycyclic aromatic hydrocarbons (ΣPAH) in the surface sediments of China’s marginal seas. BC content ranges from <0.10 to 2.45 mg/g dw (grams dry weight) in the sediments studied, and varied among the different coastal regions. The Bohai Bay sediments had the highest BC contents (average 2.18 mg/g dw), which comprises a significant fraction (27%–41%) of the total organic carbon (TOC) preserved in the sediments. In comparison, BC in the surface sediments of the North Yellow Sea, Jiaozhou Bay, East China Sea and the South China Sea is less abundant and accounted for an average of 6%, 8%, 14% and 5%, respectively, of the sedimentary organic carbon pool. The concentration of ΣPAH in the surface sediments ranges from 41 to 3 667 ng/g dw and showed large spatial variations among the sampling sites of different costal regions. The Bohai Bay has the highest ΣPAH values, ranging from 79 to 3 667 ng/g dw. This reflects the high anthropogenically contaminated nature of the sediments in the bay. BC is positively correlated to TOC but a strong correlation is not found between BC and ΣPAH in the surface sediments studied, suggesting that BC and PAHs preserved in the sediments are derived from different sources and controlled by different biogeochemical processes. Our study suggests that the abundance of BC preserved in the sediments could represent a significant sink pool of carbon cycling in China’s marginal seas.

Mechanism Study of Transport and Distributions of Trace Metals in the Bohai Bay, China

This study shows that there are two regions with high trace metal concentrations in the Bohai Bay, China. The numerical hydrodynamic model coupled with geochemical analysis was applied to understand the mass transport and sedimentation in the bay. The modeling results show that the two regions are located within the residual current vortexes. Results from the particle-tracking model indicate that the trace metals released from the land sources enter the regions and take a relatively long residence time in the vortexes. The sediment radionuclide data indicates that the two regions experienced continuous and high sedimentation, and trace metals are prone to deposit in the regions. The correlations among trace metals, residual currents and radionuclides data suggest that the tides are the governing factor controlling the distributions of the trace metals in the bay. The consistence among these results also supports the reliabilities of the numerical simulation results of water and trace metal transport in this study.

Inhibition of Herpes Simplex Virus-1 by the Modified Green Tea Polyphenol EGCG-Stearate

Epigallocatechin gallate (EGCG), a green tea polyphenol possesses antioxidant, antibacterial, anticancer and antiviral properties. EGCG-Stearate (EGCG-S) is of interest for this study because of its stability and lipophilic properties. The chemical modification of EGCG-S increased its lipid solubility. Herpes simplex virus-1 (HSV-1), a member of the family Herpesviridae, and Alphaherpesvirinae subfamily is a leading cause of human viral diseases in the United States. In this study, 25 μM, 50 μM, 75 μM, and 100 μM of EGCG and EGCG-S were used to carry out cytotoxicity, cell viability and cell proliferation assays to determine the maximum non-cytotoxic concentrations on cultured A549 cells. The results suggested that 75 μM of EGCG and EGCG-S is the appropriate concentration to further study the effect on the infection of HSV-1 in A549 cells. Infectivity, antiviral, and inverted microscopy assays were performed to study the effects of EGCG and EGCG-S on HSV-1 infection. An antiviral assay was performed using luminescence and it indicated that EGCG-S treated HSV-1 showed up to 90% inhibition. Confocal microscopy images further supported the inhibitory effects of 75 μM EGCG-S on HSV-1 infection in A549 cells. The long-term goal of this research is to use EGCG-S as a possible novel topical therapeutic treatment to limit the spread of HSV-1 infections.

Quintic spline smooth semi-supervised support vector classification machine

A semi-supervised vector machine is a relatively new learning method using both labeled and unlabeled data in classifi- cation. Since the objective function of the model for an unstrained semi-supervised vector machine is not smooth, many fast opti- mization algorithms cannot be applied to solve the model. In order to overcome the difficulty of dealing with non-smooth objective functions, new methods that can solve the semi-supervised vector machine with desired classification accuracy are in great demand. A quintic spline function with three-times differentiability at the ori- gin is constructed by a general three-moment method, which can be used to approximate the symmetric hinge loss function. The approximate accuracy of the quintic spiine function is estimated. Moreover, a quintic spline smooth semi-support vector machine is obtained and the convergence accuracy of the smooth model to the non-smooth one is analyzed. Three experiments are performed to test the efficiency of the model. The experimental results show that the new model outperforms other smooth models, in terms of classification performance. Furthermore, the new model is not sensitive to the increasing number of the labeled samples, which means that the new model is more efficient.

Optimization of axial symmetrical FGM under the transient-state temperate field

A numerical procedure for analyzing the temperature distribution in a hollow axisymmetric cylinder, made of functionally gradient material （FGM）, was investigated. Based on the thermal elasticity theory and the arbitrary difference precise integration （ADPI） method, temperature distribution through the FGM cylinder in the ring section under a transient-state temperature field was developed and presented. A genetic algorithm （GA） was applied to the thermal stress optimal design of an FGM hollow cylinder, and as a result, the minimum thermal stress distribution in the FGM cylinder was obtained. A corresponding numerical procedure regarding to a ceramic-metal FGM cylinder was perforrned. and the comnutational results were discussed.

Modified Green Tea Polyphenols, EGCG-S and LTP, Inhibit Endospore in Three <i>Bacillus</i>spp.

Endospores have the ability to withstand extreme temperature, desiccation, ultraviolet radiation and chemicals which make them a threat to the food and healthcare industry. Green tea polyphenols (GTP), contain anti-microbial and anti-spore properties but not stable. In this study, two modified lipophilic green tea polyphenols, epigallocatechin-3-gallate-sterate (EGCG-S) and crude lipophilic green tea polyphenols (LTP), were used to compare their anti-spore effect with EGCG and crude GTP. Purified endospores from Bacillus cereus (B. cereus), B. megaterium and B. subtilis were treated with 1% or 5% of four tea polyphenols. Log reduction showed colony forming units (CFU) reduced significantly in all treated samples, ranging from 1.27 to 4.31 with no survivals (CFU = 0) in four samples (P < 0.05). Average percentage of inhibition for these poly-phenols treatment ranged from 91.68% to 100%. The EGCG-S and LTP have equal or better anti-spore activities compared with EGCG and GTP. EGCG-S and LTP were further used to carry out time course study on B. cereus. The results indicated that 15 min of treatment of 1% and 5% LTP and EGCG-S are able to inhibit 98.7% to 100% of germination. Transmission and scanning electron microscopy studies showed that EGCG-S caused surface disruption and damaged spores structural integrity. EGCG-S and LTP are stable anti-spore agents may aid in preventing food and beverage spoilage caused by spore-forming bacteria as well as preventing contamination in the medical industry.

Quantitative analysis of trace levels of β-ionone in water by liquid-liquidphase extraction-gas chromatography-mass spectrometry(LLE-GC-MS)

A simple and rapid technique based on liquid-liquid extraction coupled to gas chromatography-mass spectrometric detection(LLE-GC-MS) was developed for analysis of taste and odour compound β-ionone in water. Instrument parameters including programmed oven temperature, injection temperature and ion source temperature were evaluated and optimized. Effects of extraction time, ionic strength and p H on the detection efficiency were investigated and optimum conditions were 8 min of extraction time, without Na Cl addition at p H=9. Good linearity(R2=0.9997) was obtained when the linear range was 10-500 μg/L. The recoveries of β-ionone in ultrapure water and tap water samples were 88%-95% and 110%-114%, respectively. The relative standard deviations(RSD) were less than 10%. The method detection limit(MDL) and rejection quality level(RQL) were achieved at1.98 μg/L and 6.53 μg/L, respectively. LLE-GC-MS was demonstrated to be a rapid and convenient method for the determination ofβ-ionone in water samples.

Inhibition of Curcumin-Treated Herpes Simplex Virus 1 and 2 in Vero Cells

The purpose of this study was to investigate the effect of curcumin-treated Herpes simplex virus-1 (HSV-1) and Herpes simplex virus-2 (HSV-2) virions in cultured Vero cells. Previous studies have indicated that curcumin, a polyphenol extracted from the plant Curcuma longa, has demonstrated antiviral properties against a variety of viruses. After establishing the maximum non-cytotoxic concentrations of curcumin on Vero cells, HSV-1 and HSV-2 virions were treated with varying concentrations of curcumin. The effect on infectivity was determined by antiviral assays, using WST-1, plaque assays, adsorption and penetration assays. Treating HSV-1 and HSV-2 viruses with curcumin, at a concentration of 30 μM, reduces the production of infectious HSV-1 and HSV-2 virions in cultured Vero cells by interfering with the adsorption process. These results support the potential of curcumin to be used as a therapeutic agent to reduce the transmission of HSV-1 and HSV-2.

Short-and long-term sediment transport in western Bohai Bay and coastal areas

沉积核心(锝 ? 0 鈥 ? 00 厘米) 在西方的 Bohai 海湾， Haihe 河河口， Yongding 河河口和天津港口在 12 个地点被收集，中国在 24 鈥 ?2007 年 7 月 6 日期间，并且为 7Be 和 210Pb 活动分析了。由于从挖出活动的局部性的水动力学模式和经常的骚乱，不变的沉积特征没在这研究被观察。是在 7Be 和 210Pb 侧面示威了，这些放射性核种的时间、空间的变化在学习区域支持非稳定的州的 depositional 环境。由大气的来源在沉积把 7Be 和 210Pb 库存与那些作比较，我们发现了那：1 ) 从天津港口挖出或从附近的河口和沿海的区域侵蚀了的沉积为相对短的间隔(几个月) 在西方的 Bohai 海湾被保留，在西方的 Bohai 海湾在相对高的 7Be 库存思考了；2 ) 在上长期(到十年的年) ，在沉积的 210Pb 库存暗示有一张网沉积的向陆的运输，和沉积在全部学习区域是平衡团的。总的来说，我们的结果建议尽管有本地可变性，沉积由于人的活动在沉积动力学和骚乱在河口和西方的 Bohai 海湾被保留。关键词 Bohai 海湾 - 河口 - 沉积 - 沉积运输 - 放射性核种由新泽西海资助支持了(号码 6560-0000， H. 冯) 并且天津科技委员会(号码 06YFGHHZ01500 )