The purpose of this study is to investigate the function of a novel potassium transporter gene (NrHAK1) isolated from Nicotiana rustica roots using yeast complement and real-time PCR technique. The complementary DNA (...The purpose of this study is to investigate the function of a novel potassium transporter gene (NrHAK1) isolated from Nicotiana rustica roots using yeast complement and real-time PCR technique. The complementary DNA (cDNA) of NrHAK1, 2 488 bp long, contains an open reading frame (ORF) of 2 334 bp encoding a protein of 777 amino acids (87.6 kDa) with 12 predicted transmembrane domains. The NrHAK1 protein shows a high sequence similarity to those of high-affinity potassium transporters in Mesembryanthemum, Phytolacca acinosa, Arabidopsis thaliana, and so on. We found that the NrHAK1 gene could complement the yeast-mutant defect in K+ uptake. Among several tissues surveyed, the expression level of NrHAK1 was most abundant in the root tip and was up-regulated when exposed to potassium starvation. Moreover, the transcript accumulation was significantly reduced by adding 5 mmol/L NH4+ to the solution. These results suggest that NrHAK1 plays an important role in potassium absorption in N. rustica.展开更多
Glyphosate is a non-selective broad-spectrum herbicide that blocks plant growth by inhibiting 5-Enolpyruvylshikimate-3-phosphate synthase(EPSPS),a key enzyme of the shikimate pathway in microorganisms and plants.The f...Glyphosate is a non-selective broad-spectrum herbicide that blocks plant growth by inhibiting 5-Enolpyruvylshikimate-3-phosphate synthase(EPSPS),a key enzyme of the shikimate pathway in microorganisms and plants.The full-length epsps cDNA sequence(paepsps,Genebank:KY860582.1)was cloned and characterized for the first time from Phragmites australis.The full-length cDNA of paepsps was 1308 bp encoding a polypeptide of 435 amino acids.The bioinformatic analyses showed that PaEPSPS has highly homologous with EPSPS from other plants.RT-PCR analysis of paepsps expression indicated that the gene expressed in leaves,stems,and roots,with higher expression in leaves.The expression of the paepsps gene increased with glyphosate application.In addition,the transgenic tobacco containing the paepsps gene showed glyphosate resistance in comparison with control.The novel paepsps is a good candidate gene in transgenic crops with glyphosate tolerance in the future.展开更多
基金(No. 110200101008) supported by the State Tobacco Mo-nopoly Administration, China
文摘The purpose of this study is to investigate the function of a novel potassium transporter gene (NrHAK1) isolated from Nicotiana rustica roots using yeast complement and real-time PCR technique. The complementary DNA (cDNA) of NrHAK1, 2 488 bp long, contains an open reading frame (ORF) of 2 334 bp encoding a protein of 777 amino acids (87.6 kDa) with 12 predicted transmembrane domains. The NrHAK1 protein shows a high sequence similarity to those of high-affinity potassium transporters in Mesembryanthemum, Phytolacca acinosa, Arabidopsis thaliana, and so on. We found that the NrHAK1 gene could complement the yeast-mutant defect in K+ uptake. Among several tissues surveyed, the expression level of NrHAK1 was most abundant in the root tip and was up-regulated when exposed to potassium starvation. Moreover, the transcript accumulation was significantly reduced by adding 5 mmol/L NH4+ to the solution. These results suggest that NrHAK1 plays an important role in potassium absorption in N. rustica.
基金Heilongjiang Bayi Agricultural University Support Program for San Heng San Zong(ZRCQC201909).
文摘Glyphosate is a non-selective broad-spectrum herbicide that blocks plant growth by inhibiting 5-Enolpyruvylshikimate-3-phosphate synthase(EPSPS),a key enzyme of the shikimate pathway in microorganisms and plants.The full-length epsps cDNA sequence(paepsps,Genebank:KY860582.1)was cloned and characterized for the first time from Phragmites australis.The full-length cDNA of paepsps was 1308 bp encoding a polypeptide of 435 amino acids.The bioinformatic analyses showed that PaEPSPS has highly homologous with EPSPS from other plants.RT-PCR analysis of paepsps expression indicated that the gene expressed in leaves,stems,and roots,with higher expression in leaves.The expression of the paepsps gene increased with glyphosate application.In addition,the transgenic tobacco containing the paepsps gene showed glyphosate resistance in comparison with control.The novel paepsps is a good candidate gene in transgenic crops with glyphosate tolerance in the future.
