Long-wavelength fluorescence carbon dots (CDs) show great importance in multiple fields,especially for the biochemical sensing.Here,we proposed one type of CDs doped with nitrogen and sulfur through the hydrothermal m...Long-wavelength fluorescence carbon dots (CDs) show great importance in multiple fields,especially for the biochemical sensing.Here,we proposed one type of CDs doped with nitrogen and sulfur through the hydrothermal method,which exhibited obvious yellow-fluorescence in aqueous solution.Importantly,the fluorescence intensity of CDs decreased with pH decreasing in the acidic range,thus a linear relationship between pH and fluorescence intensity was established,exhibiting the potential of pH sensing.Additionally,introducing tigecycline into CDs resulted in their decreased fluorescence,thus,we further established a strategy of detecting tigecycline with the concentration range of 200 μM to 7 nM.Meanwhile,we elucidated the static quenching as the major mechanism for CDs responding tigecycline,which was induced by the formed new complex between CDs and tigecycline.Furthermore,the practicality of the method was verified by examining the recovery of tigecycline in the actual lake-water samples.展开更多
Colorectal cancer(CRC)is a type of malignant tumor that seriously threatens human health and life,and its treatment has always been a difficulty and hotspot in research.Herein,this study for the first time reports tha...Colorectal cancer(CRC)is a type of malignant tumor that seriously threatens human health and life,and its treatment has always been a difficulty and hotspot in research.Herein,this study for the first time reports that antipsychotic aripiprazole(Ari)against the proliferation of CRC cells both in vitro and in vivo,but with less damage in normal colon cells.Mechanistically,the results showed that5-hydroxytryptamine 2B receptor(HTR2B)and its coupling protein G protein subunit alpha q(Gaq)were highly distributed in CRC cells.Ari had a strong affinity with HTR2B and inhibited HTR2B downstream signaling.Blockade of HTR2B signaling suppressed the growth of CRC cells,but HTR2B was not found to have independent anticancer activity.Interestingly,the binding of Gaq to HTR2B was decreased after Ari treatment.Knockdown of Gaq not only restricted CRC cell growth,but also directly affected the antiCRC efficacy of Ari.Moreover,an interaction between Ari and Gaq was found in that the mutation at amino acid 190 of Gaq reduced the efficacy of Ari.Thus,these results confirm that Gaq coupled to HTR2B was a potential target of Ari in mediating CRC proliferation.Collectively,this study provides a novel effective strategy for CRC therapy and favorable evidence for promoting Ari as an anticancer agent.展开更多
基金supported by Natural Science Foundation of Chongqing,China(cstc2021jcyj-msxmx0027)Talent and Innovation Project of Beibei District in Chongqing(2022-11)Performance Incentive and Guidance Special Project of Scientific Research Organization,Chongqing Science and Technology(cstc2021jxjl130024).
文摘Long-wavelength fluorescence carbon dots (CDs) show great importance in multiple fields,especially for the biochemical sensing.Here,we proposed one type of CDs doped with nitrogen and sulfur through the hydrothermal method,which exhibited obvious yellow-fluorescence in aqueous solution.Importantly,the fluorescence intensity of CDs decreased with pH decreasing in the acidic range,thus a linear relationship between pH and fluorescence intensity was established,exhibiting the potential of pH sensing.Additionally,introducing tigecycline into CDs resulted in their decreased fluorescence,thus,we further established a strategy of detecting tigecycline with the concentration range of 200 μM to 7 nM.Meanwhile,we elucidated the static quenching as the major mechanism for CDs responding tigecycline,which was induced by the formed new complex between CDs and tigecycline.Furthermore,the practicality of the method was verified by examining the recovery of tigecycline in the actual lake-water samples.
基金supported by Chongqing basic research and frontier exploration project(cstc2022ycjh-bgzxm0119,China)。
文摘Colorectal cancer(CRC)is a type of malignant tumor that seriously threatens human health and life,and its treatment has always been a difficulty and hotspot in research.Herein,this study for the first time reports that antipsychotic aripiprazole(Ari)against the proliferation of CRC cells both in vitro and in vivo,but with less damage in normal colon cells.Mechanistically,the results showed that5-hydroxytryptamine 2B receptor(HTR2B)and its coupling protein G protein subunit alpha q(Gaq)were highly distributed in CRC cells.Ari had a strong affinity with HTR2B and inhibited HTR2B downstream signaling.Blockade of HTR2B signaling suppressed the growth of CRC cells,but HTR2B was not found to have independent anticancer activity.Interestingly,the binding of Gaq to HTR2B was decreased after Ari treatment.Knockdown of Gaq not only restricted CRC cell growth,but also directly affected the antiCRC efficacy of Ari.Moreover,an interaction between Ari and Gaq was found in that the mutation at amino acid 190 of Gaq reduced the efficacy of Ari.Thus,these results confirm that Gaq coupled to HTR2B was a potential target of Ari in mediating CRC proliferation.Collectively,this study provides a novel effective strategy for CRC therapy and favorable evidence for promoting Ari as an anticancer agent.