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Correlation of Seven Biological Factors(Hsp90α,p53,MDM2,Bcl-2,Bax,Cytochrome C,and Cleaved caspase3)with Clinical Outcomes of ALK+ Anaplastic Large-cell Lymphoma 被引量:9
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作者 LI Hui Ling HUANG Xue Ping +6 位作者 ZHOU Xin Hua JI Tian Hai WU Zi Qing WANG Zhi Qiang JIANG Hui Yong LIU Fan Rong ZHAO Tong 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2011年第6期630-641,共12页
Objective To explore correlation of seven apoptosis-related proteins (Hsp9Oa, p53, MDM2, Bcl-2, Bax, Cytochrome C, and Cleaved caspase3) with clinical outcomes of ALK+ anaplastic large-cell lymphoma (ALCL). Metho... Objective To explore correlation of seven apoptosis-related proteins (Hsp9Oa, p53, MDM2, Bcl-2, Bax, Cytochrome C, and Cleaved caspase3) with clinical outcomes of ALK+ anaplastic large-cell lymphoma (ALCL). Methods Using immunohistochemistry and immunofluorescence double staining methods, the expressions of these seven apoptosis-associated proteins were studied to clarify their relationship with clinical outcomes of 36 ALK+ and 25 ALK- systemic ALCL patients enrolled between 1996 and 2006. The relationship of these apoptosis-regulating proteins with NPM-ALK status was also evaluated with the tyrosine inhibitor herbimycin A (HA) in vitro by immunocytochemistry, Western blotting and flow cytometric assays. Results The presence of Hsp90a-, MDM2-, Bax-, Cytochrome C, and Cleaved caspase3-positive tumor cells was found significantly different in ALK+ and ALK- ALCLs , which was correlated with highly favorable clinical outcome. The Bcl-2- and p53-positive tumor cells were found in groups of patients with unfavorable prognosis. Inhibition of NPM-ALK by HA could reactivate the p53 protein and subsequent apoptosis-related proteins and therefore induced apoptosis in ALK+ ALCL cells. Conclusion Our results suggest that these seven proteins might be involved in apoptosis regulation and associated with clinical outcome of ALK+ systemic ALCLs. We also reveal a dynamic chain relation that NPM-ALK regulates p53 expression and subsequent apoptosis cascade in ALK+ ALCLs. 展开更多
关键词 ALK+ALCL NPM-ALK Prognostic factors Apoptosis Herbimycin A
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Electroacupuncture Intervention Combined with Rat Nerve Growth Factor on Expression of Nestin and NeuN in MCAO Rats
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作者 谭峰 陈晶 +6 位作者 王健 顾勇 谭玖清 詹杰 程南方 顾明华 粱艳桂 《新中医》 CAS 2017年第1期232-237,共6页
Objective:To observe the effect of electroacupuncture(EA) on the expression of Nestin and Neuron specific nuclear protein antigen(neuron-specific protein,NeuN) at different time points after cerebral ischemia reperfus... Objective:To observe the effect of electroacupuncture(EA) on the expression of Nestin and Neuron specific nuclear protein antigen(neuron-specific protein,NeuN) at different time points after cerebral ischemia reperfusion injury.Methods:One hundred and fifty SD male rats were used to generate a one-side middle cerebral artery occlusion(MCAO) model using thread embolism method,then randomly divided them into the sham operation group,MCAO group,EA group,Nerve Growth Factor(NGF) group and EA+NGF group.The three benzene chloride tetrazolium(TTC) staining was used to detect the volume of cerebral infarction in the rats.Immunohistochemical staining was used to observe the expression of immune positive cell markers such as endogenous neural stem cell(NSC) marker protein Nestin,bromodeoxyuridine(Brdu) and NeuN on 1,7 and 14 days after cerebral ischemia in rats,and compared with the sham operation group.Results:TTC detection of the cerebral infarction volume showed that the nerve function injury score of the EA+ NGF group was significantly lower than MCAO group(P < 0.01).Ischemia led to severe loss of brain function,and EA+ NGF helped in the recovery of cerebral ischemia.Immunohistochemical results showed almost no expression of Nestin in the sham operation group;positive cells was expressed significantly higher Nestin on the 7th day(P < 0.05);then was peaked on the 14 th day.Nestin/BrdU labeled cells in the sham operation group showed minor expression,which was increased on the 7th day(P < 0.05) and was peaked on the 14 th day after ischemia.The differences were statistically significant on the 7th and 14 th day among the EA group,NGF group,EA+NGF group and MCAO group(P< 0.05).NeuN expression was higher in the sham operation group,and was increased on the 7th day after ischemia.NeuN/BrdU labeled cells showed higher expression in the sham operation group,the most obvious improvement was in the EA group,and the NGF and EA+NGF groups also showed significantly increased expression,while MCAO group showed the least.Conclusion:Protective effect of EA combined with NGF on cerebral ischemia reperfusion injury may induce nerve cell regeneration,accelerate proliferation of newborn cells,and promote differentiation of newborn cells,which is important for the recovery of nerve function. 展开更多
关键词 中医 症状 临床治疗 患者
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Morphology of spinal cord extracellular matrix-derived acellular scaffolds fabricated in rats 被引量:1
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作者 Wenhua Yin Kaiwu Lu Dadi Jin 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第10期767-771,共5页
Acellular peripheral allograft scaffolds can be fabricated using chemical extraction techniques,but methods for producing acellular scaffold derived from spinal cord tissue are not currently available.The present stud... Acellular peripheral allograft scaffolds can be fabricated using chemical extraction techniques,but methods for producing acellular scaffold derived from spinal cord tissue are not currently available.The present study demonstrated that chemical extraction using Triton X-100 and sodium deoxycholate could be used to completely remove the cells,axons and neural sheaths in spinal cord extracellular matrix-derived scaffolds.The matrix fibers were longitudinally arranged in a wave-like formation,and were connected by fiber junctions.Lattice-shaped fiber cages appeared and developed into bone trabecula-like changes.The natural structure of matrix fibers in the scaffolds was maintained;this helps to guide the differentiation and migration of implanted stem cells.Decellularized spinal cord extracellular matrix-derived scaffolds can provide an ideal substance for fabricating tissue-engineered spinal cord. 展开更多
关键词 spinal cord extracellular matrix DECELLULARIZATION scaffolds neural regeneration
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GABPB1-AS1 acts as a tumor suppressor and inhibits non-small cell lung cancer progression by targeting miRNA-566/F-box protein 47
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作者 HUALIANG LV CHANGCHUN LAI +1 位作者 WENQU ZHAO YIBO SONG 《Oncology Research》 SCIE 2021年第6期401-409,共9页
It has been certified that GABPB1-AS1 is aberrantly expressed and plays as a vital role in some kinds of cancers.However,its expression pattern and functions in non-small cell lung cancer(NSCLC)are still largely unknow... It has been certified that GABPB1-AS1 is aberrantly expressed and plays as a vital role in some kinds of cancers.However,its expression pattern and functions in non-small cell lung cancer(NSCLC)are still largely unknown.This study aims to assess GABPB1-AS1 expression and biological roles in NSCLC.The expression of GABPB1-AS1 was detected in NSCLC specimens and adjacent normal specimens.CCK8 and Transwell assays were performed to evaluate the effects of GABPB1-AS1 on NSCLC cell proliferation,migration and invasion.Bioinformatics tools and luciferase reporter assays were applied to predict and verify GABPB1-AS1’s direct targets.The results revealed that GABPB1-AS1 is sharply reduced in NSCLC specimens and cell lines.CCK8 assays indicated that overexpression of GABPB1-AS1 dramatically reduced NSCLC cell growth,and Transwell assays proved that NSCLC cell migration and invasion were distinctly inhibited by GABPB1-AS1.Exploration of the mechanism uncovered that miRNA-566(miR-566)/F-box protein 47(FBXO47)is directly targeted by GABPB1-AS1 in NSCLC.The study demonstrated that GABPB1-AS1 inhibited NSCLC cell proliferation,migration and invasion by targeting miR-566/FBXO47. 展开更多
关键词 GABPB1-AS1 NSCLC PROGRESSION miRNA-566 FBXO47
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Long-term follow-up of haploidentical transplantation in relapsed/refractory severe aplastic anemia:a multicenter prospective study 被引量:14
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作者 Lan-Ping Xu Zheng-Li Xu +10 位作者 Shun-Qing Wang De-Pei Wu Su-Jun Gao Jian-Min Yang Ling-Hui Xia Qi-Fa Liu Ming Jiang Hai Bai Xi Zhang Xin Wang Xiao-Jun Huang 《Science Bulletin》 SCIE EI CSCD 2022年第9期963-970,M0004,共9页
In recent decades,haploidentical stem cell transplantation(haplo-SCT)to treat severe aplastic anemia(SAA)has achieved remarkable progress.However,long-term results are still lacking.We conducted a multicenter prospect... In recent decades,haploidentical stem cell transplantation(haplo-SCT)to treat severe aplastic anemia(SAA)has achieved remarkable progress.However,long-term results are still lacking.We conducted a multicenter prospective study involving SAA patients who underwent haplo-SCT as salvage therapy.Long-term outcomes were assessed,mainly focusing on survival and quality of life(QoL).Longitudinal QoL was prospectively evaluated during pretransplantation and at 3 and 5 years posttransplantation using the SF-36 scale in adults and the PedsQL 4.0 scale in children.A total of 287 SAA patients were enrolled,and the median follow-up was 4.56 years(range,3.01–9.05 years)among surviving patients.During the long-term follow-up,268 of 275 evaluable patients(97.5%)obtained sustained full donor chimerism,and 93.4%had complete hematopoietic recovery.The estimated overall survival and failure-free survival for the whole cohort at 9 years were 85.4%±2.1%and 84.0%±2.2%,respectively.Age(≥18 years)and a poorer performance status(ECOG>1)were identified as risk factors for survival outcomes.For Qo L recovery after haplo-SCT,we found that QoL progressively improved from pretransplantation to the 3-year and 5-year time points with statistical significance.The occurrence of chronic graft versus host disease was a risk factor predicting poorer QoL scores in both the child and adult cohorts.At the last followup,74.0%of children and 72.9%of adults returned to normal school or work.These inspiring long-term outcomes suggest that salvage transplantation with haploidentical donors can be routine practice for SAA patients without human leukocyte antigen(HLA)-matched donors. 展开更多
关键词 Aplastic anemia HAPLOIDENTICAL Long-term follow-up Quality of life
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Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections: a multicenter study in China 被引量:21
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作者 LI De-zhi CHEN Yu-sheng +19 位作者 YANG Jing-ping ZHANG Wei HU Cheng-ping LI Jia-shu MU Lan HU Ying-hui GENG Rong HU Ke CAI Shao-xi WAN Huan-ying WANG Qiu-yue WEI Li-ping DU Juan YU Qin ZHONG Xiao-ning WANG Rui-qin MA Jian-jun TIAN Gui-zhen WANG Si-qin GAO Zhan-cheng 《Chinese Medical Journal》 SCIE CAS CSCD 2011年第5期687-692,共6页
Background Staphylococcus aureus (S. aureus) remains as an important microbial pathogen resulting in community and nosocomial acquired infections with significant morbidity and mortality. Few reports for S. aureus i... Background Staphylococcus aureus (S. aureus) remains as an important microbial pathogen resulting in community and nosocomial acquired infections with significant morbidity and mortality. Few reports for S. aureus in lower respiratory tract infections (LRTIs) have been documented. The aim of this study was to explore the molecular epidemiology of S. aureus in LRTIs in China.Methods A multicenter study of the molecular epidemiology of S. aureus in LRTIs was conducted in 21 hospitals in Beijing, Shanghai and twelve other provinces from November 2007 to February 2009. All the collected S. aureus strains were classified as minimum inhibitory concentration (MIC), mecA gene, virulence genes Panton-Valentine Leukocidin (PVL) and y-hemolysin (hlg), staphylococcal cassette chromosome mec (SCCmec) type, agr type, and Multilocus Sequence Typinq (MLST).Results Totally, nine methicillin-sensitive S. aureus (MSSA) and 29 methicillin-resistant S. aureus (MRSA) strains were isolated after culture from a total of 2829 sputums or bronchoalveolar lavages. The majority of MRSA strains (22/29) had a MIC value of 〉512 μg/ml for cefoxitin. The mecA gene acting as the conservative gene was carried by all MRSA strains. PVL genes were detected in only one S. aureus strain (2.63%, 1/38). The hlg gene was detected in almost the all S. aureus (100% in MSSA and 96.56% in MRSA strains). About 75.86% of MRSA strains carried SCCmec Ⅲ. Agr type 1 was predominant (78.95%) among the identified three agr types (agr types 1,2, and 3). Totally, ten sequence type (ST) of S. aureus strains were detected. A new sequence type (ST1445) was found besides confirming ST239 as the major sequence type (60.53%). A dendrogram generated from our own MLST database showed all the bootstrap values 〈50%. Conclusion Our preliminary epidemiology data show SCCmec Ⅲ, ST239 and agr type 1 of S. aureus as the predominant strains in LRTIs in Mainland of China. 展开更多
关键词 Staphylococcus aureus lower respirato tract infections molecular epidemiology staphylococcal cassette chromosome mec Multilocus Sequence Typing
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Haploidentical hematopoietic cell transplantation for severe acquired aplastic anemia: a case-control study of post-transplant cyclophosphamide included regimen vs. anti-thymocyte globulin & colony-stimulating factor-based regimen 被引量:3
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作者 Lanping Xu Bin Fu +13 位作者 Wenjing Wang Yajing Xu Depei Wu Shunqing Wang Qifa Liu Linghui Xia Sujun Gao Ming Jiang Jianmin Wang Xi Zhang Hai Bai Huiren Chen Chunfu Li Xiaojun Huang 《Science China(Life Sciences)》 SCIE CAS CSCD 2020年第6期940-942,共3页
Dear Editor,Haploidentical allogeneic hematopoietic stem cell transplantation(haplo-HSCT),a curative therapy for severe aplastic anemia(SAA)patients,has been used clinically for decades.Two models,not involving ex vit... Dear Editor,Haploidentical allogeneic hematopoietic stem cell transplantation(haplo-HSCT),a curative therapy for severe aplastic anemia(SAA)patients,has been used clinically for decades.Two models,not involving ex vitro T-cell depletion,have been adopted for haplo-HSCT in patients with SAA.The first is referred to as the"Beijing protocol"(Xu et al.,2017),and comprises a conditioning regimen using busulfex(BU),cyclophosphamide(CY). 展开更多
关键词 CSF anti-thymocyte globulin colony-stimulating factor-based regimen Haploidentical hematopoietic cell transplantation for severe acquired aplastic anemia a case-control study of post-transplant cyclophosphamide included regimen vs
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Drug-resistant genes carried by Acinetobacter baumanii isolated from patients with lower respiratory tract infection 被引量:13
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作者 DAI Ning LI De-zhi +24 位作者 CHEN Ji-chao CHEN Yu-sheng GENG Rong HU Ying-hui YANG Jing-ping DU Juan HU Cheng-ping ZHANG Wei LI Jia-shu YU Qin WAN Huan-ying MU Lan ZHONG Xiao-ning WEI Li-ping MA Jian-jun WANG Qiu-yue HU Ke TIAN Gui-zhen CAI Shao-xi WANG Rui-qin HE Bei WANG Si-qin WANG Zhan-wei ZHAO Su-rui GAO Zhan-cheng 《Chinese Medical Journal》 SCIE CAS CSCD 2010年第18期2571-2575,共5页
Background Acinetobacter baumanii (A. baumanii ) remains an important microbial pathogen resulting in nosocomialacquired infections with significant morbidity and mortality. The mechanism by which nosocomial bacteri... Background Acinetobacter baumanii (A. baumanii ) remains an important microbial pathogen resulting in nosocomialacquired infections with significant morbidity and mortality. The mechanism by which nosocomial bacteria, like A. baumanii, attain multidrug resistance to antibiotics is of considerable interest. The aim in this study was to investigate the spread status of antibiotic resistance genes, such as multiple 13-1actamase genes and aminoglycoside-modifying enzyme genes, from A. baumanii strains isolated from patients with lower respiratory tract infections (LRTIs). Methods Two thousand six hundred and ninety-eight sputum or the bronchoalveolar lavage samples from inpatients with LRTIs were collected in 21 hospitals in the mainland of China from November 2007 to February 2009. All samples were routinely inoculated. The isolated bacterial strains and their susceptibility were analyzed via VITEK-2 expert system. Several kinds of antibiotic resistant genes were further differentiated via polymerase chain reaction and sequencing methods. Results Totally, 39 A. baumanii strains were isolated from 2698 sputum or bronchoalveolar lavage samples. There was not only a high resistant rate of the isolated A. baumanfi strains to ampicillin and first- and second-generation cephalosporins (94.87%, 100% and 97.44%, respectively), but also to the third-generation cephalosporins (ceftriaxone at 92.31%, ceftazidine at 51.28%) and imipenem (43.59%) as well. The lowest antibiotic resistance rate of 20.51% was found to amikacin. The OXA-23 gene was identified in 17 strains of A. baumanii, and the AmpC gene in 23 strains. The TEM-1 gene was carried in 15 strains. PER-1 and SHV-2 genes were detected in two different strains. Aminoglycoside-modifying enzyme gene aac-3-1a was found in 23 strains, and the aac-6"lb gene in 19 strains, aac-3-1a and aac-6"lb genes hibernated in three A. baumanfi strains that showed no drug-resistant phenotype. Conclusions A. baumanii can carry multiple drug-resistant genes at the same time and result in multi-drug resistance. Aminoglycoside-modifying enzyme genes could be hibernating in aminoglycoside sensitive strains without expressing their phenotype. 展开更多
关键词 Acinetobacter baumanii lower respiratory tract infections Β-LACTAMASE drug-resistant gene
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Atorvastatin attenuates IL-6 production partly via activating heme oxygenase-1 in LPS-stimulated RAW264.7 macrophages
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作者 王晓俏 林永青 +6 位作者 陈样新 陈仲清 王景峰 聂如琼 秦再生 徐建设 古妙宁 《South China Journal of Cardiology》 CAS 2010年第1期58-65,共8页
Background Statins are known as a lipid-lowering drug as well as anti-inflammatory effect, this article aimed to evaluate the effect of atorvastatin on LPS-induced interleukin-6 (IL-6) production and determine the r... Background Statins are known as a lipid-lowering drug as well as anti-inflammatory effect, this article aimed to evaluate the effect of atorvastatin on LPS-induced interleukin-6 (IL-6) production and determine the related mechanisms in RAW264.7 macrophages. Methods The levels of IL-6 were determined by enzyme linked immunosorbent assay (ELISA). The levels of mRNA and protein expression of IL-6 and heme oxygenase-1 (HO-1) were respectively determined by quantitative PCR and western-blot. Results LPS could significantly increase mRNA expression of IL-6 and its secretion in dose- and time-dependent manners, which could be significantly attenuated by atorvastatin. In addition, HO-1 expression could be significantly increased by atorvastatin treatment, and it could be remarkably attenuated by SB203580 and PD98059 but not SP600125, which suggests that extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) pathways participate in regulating the above-mentioned effects of atorvastatin on HO-1 expression. In addition, SnPP, a kind of HO-1 activity inhibitor could significantly attenuate atorvastatin’s effects on IL-6 expression and secretion in LPS-stimulated RAW264.7 macrophages. Conclusions Atorvastatin can attenuate LPS-induced IL-6 expression and secretion by activating HO-1 via ERK and p38 MAPK pathways, which helps to explain atorvastatin has pleiotropic benefits for the treatment of diseases associated with inflammation. 展开更多
关键词 LIPOPOLYSACCHARIDE INTERLEUKIN-6 heme oxygenase-1 ATORVASTATIN mitogen-activated protein kinase
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中国胃癌肝转移患者临床诊疗特征及预后分析:全国多中心队列研究(RECORD研究) 被引量:4
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作者 高云鹤 郗洪庆 +34 位作者 商亮 唐兆庆 卫勃 乔治 唐云 王鑫鑫 周静 汪学非 黄昌明 陆俊 李国新 余江 梁延锐 季加孚 李子禹 薛侃 梁寒 柯彬 臧潞 何子锐 谢少华 黄华 徐泽宽 田艳涛 熊建平 李佶阳 崔秋霞 李力 卢婷婷 宋奇嬴 刘诗贺 孙益红 李乐平 陈凛 RECORD研究协作组 《Science Bulletin》 SCIE EI CAS CSCD 2024年第3期303-307,共5页
Despite surgical improvements and pharmacological advances,management of late-stage gastric cancer patients,especially those with hepatic metastasis remains challenging[1–3].Although nationwide registry data from SEE... Despite surgical improvements and pharmacological advances,management of late-stage gastric cancer patients,especially those with hepatic metastasis remains challenging[1–3].Although nationwide registry data from SEER(Surveillance,Epidemiology,and End Results)[4]and the Nordic database[5]in Western countries have provided epidemiological information for patients with gastric cancer liver metastasis(GCLM),little is known about the detailed clinical characteristics. 展开更多
关键词 胃癌肝转移 METASTASIS cancer
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The Chinese Society of Clinical Oncology(CSCO):clinical guidelines for the diagnosis and treatment of gastric cancer 被引量:154
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作者 Feng-Hua Wang Lin Shen +19 位作者 Jin Li Zhi-Wei Zhou Han Liang Xiao-Tian Zhang Lei Tang Yan Xin Jing Jin Yu-Jing Zhang Xiang-Lin Yuan Tian-Shu Liu Guo-Xin Li Qi Wu Hui-Mian Xu Jia-Fu Ji Yuan-Fang Li Xin Wang Shan Yu Hao Liu Wen-Long Guan Rui-Hua Xu 《Cancer Communications》 SCIE 2019年第1期75-105,共31页
China is one of the countries with the highest incidence of gastric cancer.There are differences in epidemiological characteristics,clinicopathological features,tumor biological characteristics,treatment patterns,and ... China is one of the countries with the highest incidence of gastric cancer.There are differences in epidemiological characteristics,clinicopathological features,tumor biological characteristics,treatment patterns,and drug selection between gastric cancer patients from the Eastern and Western countries.Non-Chinese guidelines cannot specifically reflect the diagnosis and treatment characteristics for the Chinese gastric cancer patients.The Chinese Society of Clinical Oncology(CSCO)arranged for a panel of senior experts specializing in all sub-specialties of gastric cancer to compile,discuss,and revise the guidelines on the diagnosis and treatment of gastric cancer based on the findings of evidence-based medicine in China and abroad.By referring to the opinions of industry experts,taking into account of regional differences,giving full consideration to the accessibility of diagnosis and treatment resources,these experts have conducted experts’consensus judgement on relevant evidence and made various grades of recommendations for the clinical diagnosis and treatment of gastric cancer to reflect the value of cancer treatment and meeting health economic indexes.This guideline uses tables and is complemented by explanatory and descriptive notes covering the diagnosis,comprehensive treatment,and follow-up visits for gastric cancer. 展开更多
关键词 Chinese Society of Clinical Oncology(CSCO) Gastric cancer Diagnosis Surgery NEOADJUVANT ADJUVANT RADIOTHERAPY Chemotherapy Targeted therapy IMMUNOTHERAPY
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