The changes of chlorophyll and malondialde-hyde (MDA) contents, plasma membrane permeability confirmed that 0.1 and 1 mmol/L sodium nitroprusside (SNP), a donor of nitric oxide (NO) in vivo, could markedly alleviate t...The changes of chlorophyll and malondialde-hyde (MDA) contents, plasma membrane permeability confirmed that 0.1 and 1 mmol/L sodium nitroprusside (SNP), a donor of nitric oxide (NO) in vivo, could markedly alleviate the oxidative damage to wheat (Triticum aestivum L.) leaves induced by 150 and 300 mmol/L NaCl treatments, respectively. Further results proved that NO significantly enhanced the activities of superoxide dismutase (SOD) and catalase (CAT), both of which separately contributed to the delay of O2- and H2O2 accumulation in wheat leaves under saltstress. Meanwhile, the accumulation of proline was apparently accelerated. Therefore, these results suggested that NO could strongly protect wheat leaves from oxidative damage caused by salt stress.展开更多
Soybean mosaic virus （SMV） is a severe disease in worldwide soybean production. A cross was made between Kefeng No.1 with broad spectrum resistance to SMV and Nannong 1138-2, a susceptible cultivar. The inheritance ...Soybean mosaic virus （SMV） is a severe disease in worldwide soybean production. A cross was made between Kefeng No.1 with broad spectrum resistance to SMV and Nannong 1138-2, a susceptible cultivar. The inheritance of resistance to SMV strain Sa prevailing in southern China was analyzed. Results of x<sup>2</sup> test from inoculation experiment on parents F1, F2 and F3 lines showed that the resistance to strain Sa was controlled by a single dominant gene Rsa. BSA method was adopted and 900 random 10-mer primers were used to amplify total DNA from resistant pool and susceptible pool in order to obtain polymorphic bands in two bulks.16 primers could generate polymorphic bands, of which OPW-05 and OPAS-06 could generate the most stable RAPD patterns. RAPD markers OPW-05<sub>660</sub> and OPAS-06<sub>1800</sub> were found to be linked to Rsa.Their order and genetic distance were OPAS-06（1800）22.2 cM Rsa 10.1 cM OPW-05<sub>660</sub>. Southern blotting showed that both OPAS-06<sub>1800</sub> and OPW-05<sub>660</sub> were low copy DNA in genomic DNA. OPW-05<sub>(</sub>展开更多
Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsible for induction of embryonic diapause in many insects. Using rapid amplification of the cDNA ends (RACE) method, the...Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsible for induction of embryonic diapause in many insects. Using rapid amplification of the cDNA ends (RACE) method, the cDNA encoding diapause hormone in Helicoverpa armigera, a main kind of crops pest was cloned. The nucleotide sequence reveals that the mRNA encodes an open reading frame and the 25-aa DH peptide is localized at N-terminal region just after the signal peptide. A homology search showed that H. armigera DH has high homology with the diapause hormone of Bornbyx mori and Helicoverpa zea; and it also belongs to the FXPRL neuropeptide family. Thus, H. armigera DH seems to be a new type of diapause hormone molecule.展开更多
Current plant functional genomics is converging on two as- pects to provide sustainable solutions to cater to the demands of the growing population： 1） engineering crops for sustainable food security, where recently...Current plant functional genomics is converging on two as- pects to provide sustainable solutions to cater to the demands of the growing population： 1） engineering crops for sustainable food security, where recently identified CRISPR/Cas is playing a detrimental role （Belhaj et al., 2014） and 2） identifying regulators of the post-transcriptional regulation, which can be functionally engineered. Canonical splicing has been widely seen and associated with functional protein diversity in plants （Min et al., 2015） （Figure 1）. Concurrent patterns of exonic and intronic splicing have revealed several new isoforms,展开更多
A scanning electron microscope observation is carried out on Glycine soja L., which grows around the estuary of the Yellow River, in Kenli County, Shandong Province. It is first discovered that there are salt glands, ...A scanning electron microscope observation is carried out on Glycine soja L., which grows around the estuary of the Yellow River, in Kenli County, Shandong Province. It is first discovered that there are salt glands, in the shape of balls, on the surface of the stems and leaves of G. soja L., and distributed at the intercellular layer. With a handle at the base, the salt glands are not equal in size, and show a layer-upon-layer structure. They have a diameter of 21 .6 μm, a handle length of 1.2 μm, their secretory pore’s diameter is 5.6 μm. There are two ways to secrete salt ions: younger glands secrete salt ions through the secretory pore, and mature glands by breaking themselves.展开更多
Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertilityrestoring gene（\%Rf\-i）\% of upland cotton. A total of 425 arbitrary 10mer oligonucleotide p...Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertilityrestoring gene（\%Rf\-i）\% of upland cotton. A total of 425 arbitrary 10mer oligonucleotide primers were screened on two DNA pools, bulked male fertile and sterile DNAs isolated from BC\-3 segregating population of （06132R×Simian No. 3）. Three primers produced repeatable polymorphisms between the paired bulks and their parents. DNA was extracted and amplified with these three primers for 92 plants of （Zhong 12A1×06132R）F\-2. Based on the male fertility scoring and RAPD amplification, it is found that one RAPD marker fragment designated OPV15 300 was linked with the fertilityrestoring gene （R f \-1） with a recombination value of 13.0±2.57%.展开更多
文摘The changes of chlorophyll and malondialde-hyde (MDA) contents, plasma membrane permeability confirmed that 0.1 and 1 mmol/L sodium nitroprusside (SNP), a donor of nitric oxide (NO) in vivo, could markedly alleviate the oxidative damage to wheat (Triticum aestivum L.) leaves induced by 150 and 300 mmol/L NaCl treatments, respectively. Further results proved that NO significantly enhanced the activities of superoxide dismutase (SOD) and catalase (CAT), both of which separately contributed to the delay of O2- and H2O2 accumulation in wheat leaves under saltstress. Meanwhile, the accumulation of proline was apparently accelerated. Therefore, these results suggested that NO could strongly protect wheat leaves from oxidative damage caused by salt stress.
文摘Soybean mosaic virus （SMV） is a severe disease in worldwide soybean production. A cross was made between Kefeng No.1 with broad spectrum resistance to SMV and Nannong 1138-2, a susceptible cultivar. The inheritance of resistance to SMV strain Sa prevailing in southern China was analyzed. Results of x<sup>2</sup> test from inoculation experiment on parents F1, F2 and F3 lines showed that the resistance to strain Sa was controlled by a single dominant gene Rsa. BSA method was adopted and 900 random 10-mer primers were used to amplify total DNA from resistant pool and susceptible pool in order to obtain polymorphic bands in two bulks.16 primers could generate polymorphic bands, of which OPW-05 and OPAS-06 could generate the most stable RAPD patterns. RAPD markers OPW-05<sub>660</sub> and OPAS-06<sub>1800</sub> were found to be linked to Rsa.Their order and genetic distance were OPAS-06（1800）22.2 cM Rsa 10.1 cM OPW-05<sub>660</sub>. Southern blotting showed that both OPAS-06<sub>1800</sub> and OPW-05<sub>660</sub> were low copy DNA in genomic DNA. OPW-05<sub>(</sub>
文摘Diapause hormone (DH) is a neurohormone which is secreted from suboesophageal ganglion and responsible for induction of embryonic diapause in many insects. Using rapid amplification of the cDNA ends (RACE) method, the cDNA encoding diapause hormone in Helicoverpa armigera, a main kind of crops pest was cloned. The nucleotide sequence reveals that the mRNA encodes an open reading frame and the 25-aa DH peptide is localized at N-terminal region just after the signal peptide. A homology search showed that H. armigera DH has high homology with the diapause hormone of Bornbyx mori and Helicoverpa zea; and it also belongs to the FXPRL neuropeptide family. Thus, H. armigera DH seems to be a new type of diapause hormone molecule.
文摘Current plant functional genomics is converging on two as- pects to provide sustainable solutions to cater to the demands of the growing population： 1） engineering crops for sustainable food security, where recently identified CRISPR/Cas is playing a detrimental role （Belhaj et al., 2014） and 2） identifying regulators of the post-transcriptional regulation, which can be functionally engineered. Canonical splicing has been widely seen and associated with functional protein diversity in plants （Min et al., 2015） （Figure 1）. Concurrent patterns of exonic and intronic splicing have revealed several new isoforms,
文摘A scanning electron microscope observation is carried out on Glycine soja L., which grows around the estuary of the Yellow River, in Kenli County, Shandong Province. It is first discovered that there are salt glands, in the shape of balls, on the surface of the stems and leaves of G. soja L., and distributed at the intercellular layer. With a handle at the base, the salt glands are not equal in size, and show a layer-upon-layer structure. They have a diameter of 21 .6 μm, a handle length of 1.2 μm, their secretory pore’s diameter is 5.6 μm. There are two ways to secrete salt ions: younger glands secrete salt ions through the secretory pore, and mature glands by breaking themselves.
文摘Bulked segregant analysis was employed to construct two mixed DNA pools to screen the RAPd marker linked with the fertilityrestoring gene（\%Rf\-i）\% of upland cotton. A total of 425 arbitrary 10mer oligonucleotide primers were screened on two DNA pools, bulked male fertile and sterile DNAs isolated from BC\-3 segregating population of （06132R×Simian No. 3）. Three primers produced repeatable polymorphisms between the paired bulks and their parents. DNA was extracted and amplified with these three primers for 92 plants of （Zhong 12A1×06132R）F\-2. Based on the male fertility scoring and RAPD amplification, it is found that one RAPD marker fragment designated OPV15 300 was linked with the fertilityrestoring gene （R f \-1） with a recombination value of 13.0±2.57%.
