Therapeutic mechanism of ginkgo biloba exocarp polysaccharides on gastric cancer

AIM: To study the therapeutic mechanism of Ginkgo biloba exocarp polysaccharides (GBEP) on gastric cancer.METHODS: Thirty patients with gastric cancer were treated with oral GBEP capsules. The area of tumors was measured by electron gastroscope before and after treatment, then the inhibitory and effective rates were calculated. The ultrastructures of tumor cells were examined by transmissional electron microscope. Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of human gastric cancer SGC-7901 cells.RESULTS: Compared with the statement before treatment,GBEP capsules could reduce the area of tumors, and the effective rate was 73.4 %. Ultrastructural changes of the cells indicated that GBEP could induce apoptosis and differentiation in tumor cells of patients with gastric cancer.GBEP could inhibit the growth of human gastric cancer SGC7901 cells following 24-72 h treatment in vitro at 10-320 mg/L,which was dose- and time-dependent. GBEP was able to elevate the apoptosis rate and expression of c-los gene,but reduce the expression of c-myc and bcl-2 genes also in a dose-dependent manner.CONCLUSION: The therapeutic mechanism of GBEP on human gastric cancer may relate to its effects on the expression of c-myc, bcl-2 and c-los genes, which can inhibit proliferation and induce apoptosis and differentiation of tumor cells.

Correlation of tumor-positive ratio and number of perigastric lymph nodes with prognosis of patients with surgically-removed gastric carcinoma

AIM:To evaluate the tumor-positive ratio and number of perigastric lymph nodes as prognostic factors of gastric carcinoma in surgically-treated patients.METHODS:The postoperative survival of 169 patients with gastric cancer who were performed D2 curative gastrectomy was analyzed with regard to its lymph node metastasis ratio and number.Meanwhile correlation of tumor-positive ratio and number of perigastric lymph nodes with pathological parameters of these patients was studied.RESULTS:The overall 5-year survival rate of all the patients studied was 29.6%.The 5-year cumulative survival rate in patients with 1%-20% and more than 20% of tumor-positive lymph nodes was 70.6% and 12.0% respectively,and 46.6% and 17.4% in those with 1-5 and more than 5 of tumorpositive lymph nodes respectively, which were significantly decreased with the increment of involved lymph nodes assessed by either numbers or ratio (P<0.05).Multiple stepwise regression analysis showed that both the positive ratio and number of tumor-involved lymph nodes were sensitive prognostic factors in these surgically-treated patients, which were also significantly correlated with tumor size and depth of submucosal invasion (P<0.05).CONCLUSION:Tumor-positive ratio and number of perigastric lymph nodes are associated with cancer progression and five-year survival rate, and may serve as valuable prognostic factors of gastric cancer in surgically-treated patients.

Prediction of ventricular fibrillation based on nonlinearmulti-parameter

Ventricular fibrillation (VF) caused by myo-cardial ischemia is one of the leading factors of death attrib-uted to cardiovascular diseases. It is particularly significantto predict VF and gain valuable time for clinic therapy. Five dogs are taken as the research objects and a VF model is introduced. The nonlinear characteristics of the ECGs before and after VF are investigated with nonlinear multi-parame-ter analysis methods, Gaussian kernel (GK) correlation es-timation algorithm and Lyapunov exponent estimation algo-rithm. Correlation entropy h2 is also presented. The results indicate that there are three parameters which will change at the same time with the conditions of myocardial ischemia,and any changes of a single parameter may be caused byother factors and mislead the judgment. Multi-parameter analysis is more reliable to reveal the heart conditions, and to predict VF without misjudgments.

Clinical Study on Treatment of Simple Obesity with Acupuncture

采用针灸为主和针灸减肥仪辅助治疗的方法治疗112例单纯性肥胖患者，获得了良好的效果。结果表明，112例经1个疗程治疗，有效率为85%；84例经2个疗程治疗，有效率为92％；43例经3个疗程治疗，有效率为98％。针仪结合的疗效疗程长者优于短者，男性优于女性，年龄轻者优于长者，肥胖度轻者优于重者，病程短者优于长者，实证者优于虚证者，无并发症者优于有并发症者，无肥胖家族史者优于有肥胖家族史者。提示，早期治疗和延长治疗时间可以提高针灸减肥的疗效。