Testis-specific protease 50（TSP50） has been identified as a testis-specific protein that is expressed abnormally in most human breast cancer samples,which makes it an attractive molecular marker and a potential targ...Testis-specific protease 50（TSP50） has been identified as a testis-specific protein that is expressed abnormally in most human breast cancer samples,which makes it an attractive molecular marker and a potential target for diagnosis and therapy.In the present study,we prepared a panel of monoclonal antibodies（mAbs） with high specificity and sensitivity against TSP50 by hybridoma method and characterized them by ELISA,Western blot,immunofluroescence and immunohistochemical analyses.The results show that all of the 9 different clones can specifically bind to TSP50.The mAbs against TSP50 we generated could be good tools for both basic and clinical studies.展开更多
Fabrication of folate and iron-substituted polyoxometalate [（FeOH2）2SiWlo036] to form nanoparticles （FA-SiWFe2） has been achieved. This inorganic-organic hybrid possesses intrinsic peroxidase-like activity, which ...Fabrication of folate and iron-substituted polyoxometalate [（FeOH2）2SiWlo036] to form nanoparticles （FA-SiWFe2） has been achieved. This inorganic-organic hybrid possesses intrinsic peroxidase-like activity, which could be used in detection of cancer cells in colorimetric multiplexed immunoassay.展开更多
Crassostrea sikamea(C.sikamea)is an important edible and medicinal seafood in China.In the present study,a compound named flazin was separated and identified from the ethyl acetate extract of C.sikamea(EAECs)for the f...Crassostrea sikamea(C.sikamea)is an important edible and medicinal seafood in China.In the present study,a compound named flazin was separated and identified from the ethyl acetate extract of C.sikamea(EAECs)for the first time.In addition,the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetra zolium(MTS)assay revealed that EAECs and flazin inhibited the transformation of splenic lymphocytes in vitro.Moreover,flazin(20μg·mL^(−1))altered the populations of splenic lymphocyte subtypes.Real-time quantitative PCR(RT-qPCR)analysis and enzyme-linked immunosorbent assay(ELISA)showed that flazin suppressed the mRNA expression and secretion of TNF-αand IL-2,and reversed Concanavalin A(ConA)-induced mRNA up-regulation and protein secretion of TNF-αand IL-2.Western blot results showed that flazin reversed ConA-induced increases in p-ERK1/2 and p-p38 in splenocytes.In conclusion,flazin exhibits effective immunomodulatory function and may be useful for treating immune-related disorders,which indicates the application potential of C.sikamea as a functional food or immunomodulator.展开更多
基金Supported by the National Natural Science Foundation of China(No.30672068)the Distinguished Young Scholars Fund of Jilin Province,China(No.20050114)+1 种基金the Key Grant of Jilin Province Science & Technology Committee,China(Nos.20060923-01,200805131)the Key Grant of Changchun City Science & Technology Committee,China(No.06GG147)
文摘Testis-specific protease 50（TSP50） has been identified as a testis-specific protein that is expressed abnormally in most human breast cancer samples,which makes it an attractive molecular marker and a potential target for diagnosis and therapy.In the present study,we prepared a panel of monoclonal antibodies（mAbs） with high specificity and sensitivity against TSP50 by hybridoma method and characterized them by ELISA,Western blot,immunofluroescence and immunohistochemical analyses.The results show that all of the 9 different clones can specifically bind to TSP50.The mAbs against TSP50 we generated could be good tools for both basic and clinical studies.
基金supported by the National Natural Science Foundation of China(No.51078066)the National Innovation Experiment Program for Undergraduates(No.201210200073)
文摘Fabrication of folate and iron-substituted polyoxometalate [（FeOH2）2SiWlo036] to form nanoparticles （FA-SiWFe2） has been achieved. This inorganic-organic hybrid possesses intrinsic peroxidase-like activity, which could be used in detection of cancer cells in colorimetric multiplexed immunoassay.
基金Education Department of Jilin Province(No.JJKH20201177KJ)the Foundation of Department of Human Resources and Social Security of Jilin Province(No.2020009)the Foundation of Jilin Province Development and Reform Commission(No.2020C015).
文摘Crassostrea sikamea(C.sikamea)is an important edible and medicinal seafood in China.In the present study,a compound named flazin was separated and identified from the ethyl acetate extract of C.sikamea(EAECs)for the first time.In addition,the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetra zolium(MTS)assay revealed that EAECs and flazin inhibited the transformation of splenic lymphocytes in vitro.Moreover,flazin(20μg·mL^(−1))altered the populations of splenic lymphocyte subtypes.Real-time quantitative PCR(RT-qPCR)analysis and enzyme-linked immunosorbent assay(ELISA)showed that flazin suppressed the mRNA expression and secretion of TNF-αand IL-2,and reversed Concanavalin A(ConA)-induced mRNA up-regulation and protein secretion of TNF-αand IL-2.Western blot results showed that flazin reversed ConA-induced increases in p-ERK1/2 and p-p38 in splenocytes.In conclusion,flazin exhibits effective immunomodulatory function and may be useful for treating immune-related disorders,which indicates the application potential of C.sikamea as a functional food or immunomodulator.
