An adjusted ELISpot-based immunoassay for evaluation of SARS-CoV-2-specific T-cell responses

Like antibody evaluation,using an effective antigen‐specific T‐cell immunity assessment method in coronavirus disease 2019(COVID‐19)patients,survivors and vaccinees is crucial for understanding the immune persistence,prognosis assessment,and vaccine development for COVID‐19.This study evaluated an empirically adjusted enzyme‐linked immunospot assay for detecting severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2)‐specific T‐cell immunity in 175 peripheral blood samples from COVID‐19 convalescents and healthy individuals.Results of viral nucleic acid were used as the gold standard of infection confirmation.The SARS‐CoV‐2M peptide pool had higher sensitivity of 85%and specificity of 71%for the single peptide pool.For combined peptide pools,the parallel evaluation(at least one of the peptide pools is positive)of total peptide pools(S1&S2&M&N)had higher sensitivity(up to 93%),and the serial evaluation(all peptide pools are positive)of total peptide pools had higher specificity(up to 100%).The result of the serial evaluation was better than that of the parallel evaluation as a whole.The detection efficiency of M and N peptide pool serial evaluation appeared the highest,with a sensitivity of 80%and specificity of 93%.This T‐cell immunity detection assay introduced in this report can achieve high operability and applicability.Therefore,it can be an effective SARS‐CoV‐2‐specific cellular immune function evaluation method.

COVID-19康复者持续恢复趋势下的部分异常:一项2年随访研究

对COVID-19康复患者健康水平和康复后生活质量的评估是值得关注的重要问题.本研究建立了COVID-19康复者的随访队列,进行问卷调查和血液样本检测,血液指标共55项.参与者为2020年初大流行早期阶段湖北省麻城市的COVID-19患者(n=136),同时纳入对照人群(n=125).康复1年后,60.5%报告至少存在一种不适症状,2年后该比例下降至47.5%.在急性期异常的白细胞、血小板、胆红素等指标,1年后基本恢复正常并在2年随访时保持.部分康复者血红蛋白、肌酐等在1年后异常,但多在2年后恢复.少数康复者肌红蛋白、碱性磷酸酶等指标在康复2年仍未完全恢复正常.本研究结果提示,对于新冠康复者需要合理的健康干预措施和较长的后续行动以促进康复.