Online Effective Identification of Glycopeptide Using Liquid Chromatography Combined with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS)

The detailed glycan structural analysis of glycoprotein is amenable to glycopeptide enrichment. Here, we develop a simple, effective and economical approach to enrich glycopeptides from proteolytically digested peptide mixtures by chromatographic column packed with graphite carbon and activated charcoal (G/A-column). Glycopeptide from ovalbumin was efficiently enriched by homemade G/A-column using liquid chromatography and the structure of glycopeptide was obtained by tandem mass spectrometry using Fourier transform ion cyclotron resonance mass spectrometry. The results in this study demonstrate that G/A-column can be used to enrich N-glycolpeptides and be benefit for online identification of glycopeptide using LC-MS.

Databases and Web Tools for Cancer Genomics Study

Publicly-accessible resources have promoted the advance of scientific discovery. The era of genomics and big data has brought the need for collaboration and data sharing in order to make effective use of this new knowledge. Here, we describe the web resources for cancer genomics research and rate them on the basis of the diversity of cancer types, sample size, omics data comprehensiveness, and user experience. The resources reviewed include data repository and analysis tools; and we hope such introduction will promote the awareness and facilitate the usage of these resources in the cancer research community.

Comparative metagenomic analysis of the vaginal microbiome in healthy women

The composition of these vaginal microbiome has a significant impact on women’s health.However,few studies have characterized the vaginal microbiome of healthy Chinese women using metagenomic sequencing.Here,we carried out a comparative metagenomic analysis to survey taxonomic,functional levels,and microbial communities’genome content in healthy women’s vaginal microbiome.Overall,we observed a total of 111 species,including all dominant vaginal Lactobacillus species,such as L.iners,L.crispatus,L.gasseri,and L.jensenii.Unlike microbial taxa,several pathways were ubiquitous and prevalent across individuals,including adenosine ribonucleotides de novo biosynthesis and pyruvate fermentation to acetate and lactate Ⅱ.Notably,our diversity analysis confirmed a significant difference in healthy women from different ethnic groups.Moreover,we binned vaginal assemblies into 62 high-quality genomes,including 9 L.iners,7 A.vaginae,5 L.jensenii,and 5 L.crispatus.We identified the pan and core genomes of L.iners and A.vaginae and revealed the genetic diversity.Primary differences between strains were the hypothetical genes and mobile element-like genes.Our results provide a framework for understanding the implications of the female reproductive tract’s composition and functional potential and highlight the importance of genome-resolved metagenomic analysis to further understand the human vaginal microbiome.

Trends of Adverse Pregnancy Outcomes in a High Prevalence Region of Birth Defects——Shanxi Province,China,2007-2019

Summary What is already known about this topic?Shanxi Province in northern China has been identified as a region with the highest prevalence of birth defects nationwide.With large amounts of financial support devoted for prevention and related interventions for birth defects,huge progress has been made as a 60% decrease in its prevalence was observed from 2009 to 2014.

2013~2017年中国空气质量改善与早产疾病负担改变

Preterm birth(PTB)is a leading cause of perinatal mortality and morbidity worldwide[1].Since maternal and fetal health protection has been included in the United Nations Sustainable Development Goals,identification of modifiable risk factors for PTB is particularly crucial.The Global Burden of Disease(GBD)Study 2019 recognized fine particulate matter(PM2.5)pollution as a risk factor for decreased gestational age and low birth weight.