A single nucleotide polymorphism （SNP） of 805 bp region in the intron 6 of transfor- ming growth factor β1 （TGF-fll） gene was identified by polymerase chain reaction-single-strand composi- tion polymorphism （PCR...A single nucleotide polymorphism （SNP） of 805 bp region in the intron 6 of transfor- ming growth factor β1 （TGF-fll） gene was identified by polymerase chain reaction-single-strand composi- tion polymorphism （PCR-SSCP） in 196 sheep among Small-tailed Han sheep, Tong sheep, Tan sheep and Oula sheep. Comparative sequence analysis of cloned products revealed an AGAC deletion at 294 bases up-stream of exon 7 of the TGF-βI gene （ site 14201 in gi76871756）. Statistical results of the genotype and allele frequencies in different breeds showed that gen- otype AB was dominant in the Small-tailed Han sheep. Genotype BB, however, was in majority in low- fecundity sheep. The results of a Chi-square test indi- cated that all the populations were in Hardy-Weinberg equilibrium.展开更多
基金supported by the National Natural Science Foundation of China(No.30270949)"863"Plan(No.2006AA10Z199)
文摘A single nucleotide polymorphism （SNP） of 805 bp region in the intron 6 of transfor- ming growth factor β1 （TGF-fll） gene was identified by polymerase chain reaction-single-strand composi- tion polymorphism （PCR-SSCP） in 196 sheep among Small-tailed Han sheep, Tong sheep, Tan sheep and Oula sheep. Comparative sequence analysis of cloned products revealed an AGAC deletion at 294 bases up-stream of exon 7 of the TGF-βI gene （ site 14201 in gi76871756）. Statistical results of the genotype and allele frequencies in different breeds showed that gen- otype AB was dominant in the Small-tailed Han sheep. Genotype BB, however, was in majority in low- fecundity sheep. The results of a Chi-square test indi- cated that all the populations were in Hardy-Weinberg equilibrium.
