Background: We have previously shown that the inducible kinin B1 receptor is expressed on T lymphocytes during relapses and progression in multiple sclerosis. Objective: To evaluate the correlation between the express...Background: We have previously shown that the inducible kinin B1 receptor is expressed on T lymphocytes during relapses and progression in multiple sclerosis. Objective: To evaluate the correlation between the expression of B1 receptor on peripheral blood mononuclear cells derived from patients who have multiple scle rosis with serial, clinical magnetic resonance imaging and immunological study- derived measures. Design: Using frozen samples obtained from a high-frequency m agnetic resonance imaging-immunological study, we analyzed B1 receptor messenge r RNA (mRNA) expression in peripheral blood-deriv- ed mononuclear cells serially collected from 6 patients with multiple sclerosi s and 8 healthy control subjects by semiquantitative radioactive duplex reverse transcriptasepolymerase chain reaction amplification. Timecourse kinin B1-actin mRNA ratios were subsequently compared with corresponding clinical magnetic res onance imaging and immune parameters. Results: The time-course kinin B1-actin mRNA ratio correlated positively with the Expanded Disability Status Scale index (P < .001), occurrence of clinical relapse (P=.02), volume of lesion on T2-wei ghted images (P < .003) and interleukin 2 receptor and major histocompatibility complex class II expression on CD4+lymphocytes, but not with gadoliniumenhancin g lesions. The time-course kinin B1-actin mRNA ratios were 5 to 25 times lower in samples derived from healthy controls. Conclusion: The correlation of kinin B1 receptor mRNA levels with dynamic clinical and magnetic resonance imaging mea sures suggests that expression of this receptor can serve as an index of disease activity in multiple sclerosis.展开更多
文摘Background: We have previously shown that the inducible kinin B1 receptor is expressed on T lymphocytes during relapses and progression in multiple sclerosis. Objective: To evaluate the correlation between the expression of B1 receptor on peripheral blood mononuclear cells derived from patients who have multiple scle rosis with serial, clinical magnetic resonance imaging and immunological study- derived measures. Design: Using frozen samples obtained from a high-frequency m agnetic resonance imaging-immunological study, we analyzed B1 receptor messenge r RNA (mRNA) expression in peripheral blood-deriv- ed mononuclear cells serially collected from 6 patients with multiple sclerosi s and 8 healthy control subjects by semiquantitative radioactive duplex reverse transcriptasepolymerase chain reaction amplification. Timecourse kinin B1-actin mRNA ratios were subsequently compared with corresponding clinical magnetic res onance imaging and immune parameters. Results: The time-course kinin B1-actin mRNA ratio correlated positively with the Expanded Disability Status Scale index (P < .001), occurrence of clinical relapse (P=.02), volume of lesion on T2-wei ghted images (P < .003) and interleukin 2 receptor and major histocompatibility complex class II expression on CD4+lymphocytes, but not with gadoliniumenhancin g lesions. The time-course kinin B1-actin mRNA ratios were 5 to 25 times lower in samples derived from healthy controls. Conclusion: The correlation of kinin B1 receptor mRNA levels with dynamic clinical and magnetic resonance imaging mea sures suggests that expression of this receptor can serve as an index of disease activity in multiple sclerosis.
