With the constant development of multiphoton microscopy,our ability to observe complex and dynamic biological processes deeper within living tissue,is steadily improving.Researchers use multiphoton microscopy,because ...With the constant development of multiphoton microscopy,our ability to observe complex and dynamic biological processes deeper within living tissue,is steadily improving.Researchers use multiphoton microscopy,because experiments can be conducted with little to no invasiveness or tissue damage over a long period of time with no photodamage(Mancuso et al.,2009).This allows for the introduction of tissue into the context of a three-dimensional 3D environment in which visualization of cellular activation and interaction is viable.By circumventing a distorted reconstruction with limited z-stacks,multiphoton imaging provides enhanced spatiotemporal resolution.展开更多
Microglia and macrophages in the development of maladaptive plastic changes after peripheral nerve injury:Microglia and macrophages encompass the innate immune response to injury in the central and peripheral nervous ...Microglia and macrophages in the development of maladaptive plastic changes after peripheral nerve injury:Microglia and macrophages encompass the innate immune response to injury in the central and peripheral nervous systems,respectively,and are intimately involved in the pathogenesis of maladaptive changes(Tsuda,2019).These dynamic cells can influence neuronal activity in active and quiescent states.Conflicting findings argue that peripheral macrophages facilitate the development of nerve injury-induced neuropathic pain,as opposed to central microglia(Lopes et al.,2017;Yu et al.,2020).展开更多
基金NIH grant K22NS096030(to MDB)American Pain Society Future Leaders Grant(MDB)+1 种基金Rita Allen Foundation Award in Pain(to MDB)The University of Texas System STARS program research support grant(to MDB)。
文摘With the constant development of multiphoton microscopy,our ability to observe complex and dynamic biological processes deeper within living tissue,is steadily improving.Researchers use multiphoton microscopy,because experiments can be conducted with little to no invasiveness or tissue damage over a long period of time with no photodamage(Mancuso et al.,2009).This allows for the introduction of tissue into the context of a three-dimensional 3D environment in which visualization of cellular activation and interaction is viable.By circumventing a distorted reconstruction with limited z-stacks,multiphoton imaging provides enhanced spatiotemporal resolution.
基金supported by NIH grant K22NS096030(MDB)American Pain Society Future Leaders Grant(MDB)+1 种基金Rita Allen Foundation Award in Pain(MDB)The University of Texas System STARS program research support grant(MDB).
文摘Microglia and macrophages in the development of maladaptive plastic changes after peripheral nerve injury:Microglia and macrophages encompass the innate immune response to injury in the central and peripheral nervous systems,respectively,and are intimately involved in the pathogenesis of maladaptive changes(Tsuda,2019).These dynamic cells can influence neuronal activity in active and quiescent states.Conflicting findings argue that peripheral macrophages facilitate the development of nerve injury-induced neuropathic pain,as opposed to central microglia(Lopes et al.,2017;Yu et al.,2020).
