Ribosome is one of the most abundant organelles in all living cells and plays a crucial role in cell growth. Synthesis of ribosomal components is tightly related with the change of growth conditions. We have comparati...Ribosome is one of the most abundant organelles in all living cells and plays a crucial role in cell growth. Synthesis of ribosomal components is tightly related with the change of growth conditions. We have comparatively analyzed the 5’ flanking region of ribosomal protein (RP) genes in Arabidopsis and O. sativa. In both Arabidopsis and O. sativa, there are two putative transcriptional factor binding motifs (telo box and site II elements) overrepresented in the proximal promoter region with a strong positional bias in most of the RP genes, which suggests the conserved mechanism of transcription-level control in RP genes of these two organisms. Tri-nucleotide repeats motif CTT and CCG were also common in 5’ flanking region of RP genes in Arabidopsis and O. sativa. However, we only found CCG repeat motif was enriched in O. sativa RP genes and most of them were clustered in the 5’ UTR region. This finding reveals molecular mechanism for divergent regulation of RP genes in Arabidopsis and O. sativa, and gives the possible clue to the mechanism of controlling O. sativa RP genes expression at the translation level.展开更多
Arboviral encephalitis is a group of animal and human illness that is mostly caused by several distinct families of viruses including orthobunya virus, phlebovirus, flaviviruses, and the alphaviruses. Although specifi...Arboviral encephalitis is a group of animal and human illness that is mostly caused by several distinct families of viruses including orthobunya virus, phlebovirus, flaviviruses, and the alphaviruses. Although specific signs and symptoms vary by the type of central nervous system (CNS), initial signs and symptoms are very similar. Therefore rapid immunologic and molecular tools for differential diagnosis of arboviral encephalitis viruses are important for effective case management and control of the spread of encephalitis. The qRT-PCR assay, especially multiplex PCR, has the potential to produce considerable savings in time and resources in the laboratory detection. Meanwhile, the use of IC can prevent false negatives effectively by monitoring the processes of nucleic acid extraction and amplification. This report describes the development of a panel of internally controlled multiplex one-step real-time RT-PCR assays in which two virus specific-probe sets were used in the same reaction for the detection of 15 species arboviral encephalitis viruses: the comparative sensitivity of multiplex one-step qRT-PCR assays to single plex one-step qRT-PCR assays as well as one-step RT-PCR assays for detection of each viral species. And total of 150 human serum samples were detected to evaluate the multiplex one-step qRT-PCR assays. These multiplex one-step real-time RT-PCR assays with IC were evaluated in terms of sensitivity, linearity, precision, specificity, and also field samples including serum and vector. These assays can detect and differentiate arboviral encephalitis viruses by high throughput, sensitive, and specific way. It is useful for clinical management and outbreak control of arboviral encephalitis viruses and vector surveillance.展开更多
Short Retraction Notice The paper is due to conflicts of interest. This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guideline...Short Retraction Notice The paper is due to conflicts of interest. This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. Aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused. The full retraction notice in PDF is preceding the original paper which is marked "RETRACTED".展开更多
文摘Ribosome is one of the most abundant organelles in all living cells and plays a crucial role in cell growth. Synthesis of ribosomal components is tightly related with the change of growth conditions. We have comparatively analyzed the 5’ flanking region of ribosomal protein (RP) genes in Arabidopsis and O. sativa. In both Arabidopsis and O. sativa, there are two putative transcriptional factor binding motifs (telo box and site II elements) overrepresented in the proximal promoter region with a strong positional bias in most of the RP genes, which suggests the conserved mechanism of transcription-level control in RP genes of these two organisms. Tri-nucleotide repeats motif CTT and CCG were also common in 5’ flanking region of RP genes in Arabidopsis and O. sativa. However, we only found CCG repeat motif was enriched in O. sativa RP genes and most of them were clustered in the 5’ UTR region. This finding reveals molecular mechanism for divergent regulation of RP genes in Arabidopsis and O. sativa, and gives the possible clue to the mechanism of controlling O. sativa RP genes expression at the translation level.
文摘Arboviral encephalitis is a group of animal and human illness that is mostly caused by several distinct families of viruses including orthobunya virus, phlebovirus, flaviviruses, and the alphaviruses. Although specific signs and symptoms vary by the type of central nervous system (CNS), initial signs and symptoms are very similar. Therefore rapid immunologic and molecular tools for differential diagnosis of arboviral encephalitis viruses are important for effective case management and control of the spread of encephalitis. The qRT-PCR assay, especially multiplex PCR, has the potential to produce considerable savings in time and resources in the laboratory detection. Meanwhile, the use of IC can prevent false negatives effectively by monitoring the processes of nucleic acid extraction and amplification. This report describes the development of a panel of internally controlled multiplex one-step real-time RT-PCR assays in which two virus specific-probe sets were used in the same reaction for the detection of 15 species arboviral encephalitis viruses: the comparative sensitivity of multiplex one-step qRT-PCR assays to single plex one-step qRT-PCR assays as well as one-step RT-PCR assays for detection of each viral species. And total of 150 human serum samples were detected to evaluate the multiplex one-step qRT-PCR assays. These multiplex one-step real-time RT-PCR assays with IC were evaluated in terms of sensitivity, linearity, precision, specificity, and also field samples including serum and vector. These assays can detect and differentiate arboviral encephalitis viruses by high throughput, sensitive, and specific way. It is useful for clinical management and outbreak control of arboviral encephalitis viruses and vector surveillance.
文摘Short Retraction Notice The paper is due to conflicts of interest. This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. Aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused. The full retraction notice in PDF is preceding the original paper which is marked "RETRACTED".
