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三七总皂苷对K562细胞增殖、凋亡及周期的影响及机制 被引量:9
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作者 李玉云 翟玮玮 +2 位作者 杨向荣 丁娟 阚立新 《南方医科大学学报》 CAS CSCD 北大核心 2015年第8期1103-1109,共7页
目的 研究三七总皂苷(PNS)对K562细胞增殖、凋亡、周期的影响及其相关分子机制。方法采用MTT法检测PNS对K562细胞增殖的影响;AO/EB双荧光染色法及Annexinv-FITc/PI双染色法观察细胞凋亡及死亡状况;流式细胞术检测K562细胞的周期... 目的 研究三七总皂苷(PNS)对K562细胞增殖、凋亡、周期的影响及其相关分子机制。方法采用MTT法检测PNS对K562细胞增殖的影响;AO/EB双荧光染色法及Annexinv-FITc/PI双染色法观察细胞凋亡及死亡状况;流式细胞术检测K562细胞的周期变化;RT-PCR检测mTOR信号通路主要分子mRNA的表达变化;Westernblot检测cleavedcaspase-3蛋白、cyclinD1蛋白及mTOR信号通路主要蛋白及磷酸化蛋白的表达量变化。结果100—800rtg/mL的PNS作用于K562细胞后能够抑制细胞增殖。PNS能够上调cleavedcaspase-3蛋白表达,促使K562细胞发生凋亡。并且下调cyclinD1蛋白表达,促使K562细胞阻滞在G0/G1期。同时,PNS能够抑制K562细胞mTORmRNA表达,降低mTOR蛋白和磷酸化蛋白p-mTOR(Ser2448)、p-p70S6K(Thr229/389)及P-4E-BP1(Thr37/46)的表达,从而抑制mTOR信号通路活性。结论PNS对体外培养K562细胞有抑制增殖,促进凋亡,使其发生周期阻滞的作用。其机制可能与PNS抑制mTOR信号通路活性、上调cleavedcaspase.3蛋白表达及抑制cyclinD1蛋白表达有关。 展开更多
关键词 三七总皂苷 K562细胞 增殖 凋亡 周期
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碳纳米管的力学 被引量:6
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作者 QianDong WagnerGregoryJ +5 位作者 LiuWingKam YuMinFeng RuoffRodneyS 徐志平 王立峰 郑泉水 《力学进展》 EI CSCD 北大核心 2004年第1期97-138,共42页
在发现碳纳米管后不久,对于这些有趣结构的力学性质——包括高强度、高硬度、低密度和结构的完美性的理论预测,使人们认识到它们可能具有理想的科技应用价值.对这些预测的实验验证或个别验伪以及大量基于不同模型的计算机模拟方法,使得... 在发现碳纳米管后不久,对于这些有趣结构的力学性质——包括高强度、高硬度、低密度和结构的完美性的理论预测,使人们认识到它们可能具有理想的科技应用价值.对这些预测的实验验证或个别验伪以及大量基于不同模型的计算机模拟方法,使得逾10年来对碳纳米管力学的理解日趋深入但远未达到尽头.本文回顾了理论预测,并对这种微小结构的观察和操作中经常用到的富有挑战性的实验技术进行了讨论.略述了采用的计算方法包括从头算法量子力学模拟、经典分子动力学和连续介质模型.多尺度和多物理模型的发展和模拟工具自然而然作为连接基础科学问题和工程应用的结果而出现,而这个主题仍然正在抓紧研究中.这里介绍了研究此主题的一些方法.注意力主要集中于研究力学性质的揭示方面,如杨氏模量、弯曲刚度、屈曲准则、拉伸和压缩强度.最后,讨论了利用这些性质的几个令人兴奋的应用例子,包括纳米绳束、填充的纳米管、纳米机电系统、纳米传感器和纳米管增强复合材料,引用了349篇参考文献。 展开更多
关键词 碳纳米管 力学性质 计算机模拟 杨氏模量 弯曲刚度 屈曲准则 压缩强度
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Surface diffusivity of atomic deuterium on Ni_3(Al, Ti)(110) surface with and without boron 被引量:1
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作者 J.L.Wang Y.W.Chung 《中国有色金属学会会刊:英文版》 EI CSCD 2002年第4期649-652,共4页
The electron stimulated desorption was used to measure the surface diffusivity of atomic deuterium on clean and boron modified Ni 3(Al, Ti)(110) surfaces. Boron dosing was performed using a solid state boron ion sourc... The electron stimulated desorption was used to measure the surface diffusivity of atomic deuterium on clean and boron modified Ni 3(Al, Ti)(110) surfaces. Boron dosing was performed using a solid state boron ion source. Earlier studies showed that boron dissociates water readily at temperatures as low as 130?K and that the resulting atomic hydrogen is bound to the surface strongly. The surface diffusion coefficient of atomic D on 0.05 monolayer boron modified surface was measured to be about 10 times smaller than that on the clean surface. This slower diffusion of atomic hydrogen may explain why boron improves the ductility of polycrystalline Ni 3Al in moist environments. 展开更多
关键词 氘原子 表面扩散 NI3AL Ni3Ti 金属间化合物
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Measurement of the Third-Order Nonlinear Optical Coefficient of ZnO Crystals by Using ICCD-Z-Scan 被引量:1
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作者 JIAGuang-Ming ZHANGGui-Zhong +1 位作者 XIANGWang-Hua J.B.Ketterson 《Chinese Physics Letters》 SCIE CAS CSCD 2004年第7期1356-1358,共3页
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Key Account: The Negotiating Process
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作者 PhilippeCoffre 《Chinese Business Review》 2018年第4期155-167,共13页
This research plans to evaluate the changes and evolutions from the real world of key account management compared to literature review: How decisions are taken, what processes help to be successful on tenders, how to... This research plans to evaluate the changes and evolutions from the real world of key account management compared to literature review: How decisions are taken, what processes help to be successful on tenders, how to cooperate on a world basis, what methods facilitate conclusions. This work looks for a measure of evolution and changes during a negotiation process by KAM compared to existing literature research. Key account negotiators take strategic dimensions into account when starting a negotiation process. They measure the cost of negotiations, help their clients to position themselves properly, and develop partnerships. In general, researchers are more interested in organization and clients than in integrated processes. The process analysis is built on an in-depth analysis of Key Account Management (KAM) on three matters: an in-depth interview of 20 Key Account Managers from such companies as Air Liquide, Matra, Alcatel, GEC Alstom, Bull, EADS, PPG, an advertising agency and Omnicom on prospecting, partnerships, client’s effectiveness optimization, client’s qualification, lobbies, networks, international accounts. This research shows how KAM faces complex situations, how training and monitoring bring solutions to difficult situations, how information is managed through transparency relationships, TV shows, press media, information from shows and fairs, competitors evaluation. This work conceptualizes the KAM negotiation process and permits to managers to identify the different negotiation steps from their numerous mutual professional witnesses. The number of companies could have been larger, but we interviewed each salesperson in depth. Future work on key account salespeople should consider the following subjects: influence of KAM on tenders. Which ethical steps KAM can take with client companies to improve results. Innovation perception by clients is as a tool for success. 展开更多
关键词 key accounts salespersons purchasers sales effectiveness international accounts
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价值型整合营销传播
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作者 Don·E·Schultz 何西军 黄鹂 《新营销》 2005年第3期102-104,共3页
我们的世界瞬息万变,所以很多旧的营销原则与方法一旦受到挑战,就会显得左支右绌。虽然对于很多组织来说,改革是有必要的,但却因为过去的成功而变得抗拒改革。整合营销传播就是在这种营销与媒体选项不断改变而且发展迅速的市场中诞... 我们的世界瞬息万变,所以很多旧的营销原则与方法一旦受到挑战,就会显得左支右绌。虽然对于很多组织来说,改革是有必要的,但却因为过去的成功而变得抗拒改革。整合营销传播就是在这种营销与媒体选项不断改变而且发展迅速的市场中诞生的,即20世纪80年代中后期。 展开更多
关键词 整合营销传播 IMC 营销策略 价值链管理 客户关系管理 品牌忠诚度 顾客满意度
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1例注射依那西普的患者出现斑秃复发
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作者 Posten W Swan J 李晓莉 《世界核心医学期刊文摘(皮肤病学分册)》 2005年第10期24-25,共2页
Background: Alopecia areata is a common condition of patchy hair loss that has been postulated to have an autoimmune pathogenesis involving inflammatory cytokines, including tumor necrosis factor (TNF) α. Etanercept ... Background: Alopecia areata is a common condition of patchy hair loss that has been postulated to have an autoimmune pathogenesis involving inflammatory cytokines, including tumor necrosis factor (TNF) α. Etanercept is a novel medication that blocks TNF-α-mediated processes. We report a case involving the recurrence of alopecia areata in a patient receiving etanercept. Observations: We describe a 49-year-old man with a history of rheumatoid arthritis and alopecia areata who developed a recurrence of his alopecia areata while being treated with etanercept for more than 2 years. Conclusions: The anti-TNF-αeffect of etanercept therapy may not be sufficient to prevent the recurrence of alopecia areata. The possible role of TNF-αin the pathogenesis of alopecia areata may be called into question if our observation is repeated. 展开更多
关键词 依那西普 类风湿性关节炎 自身免疫性疾病 新型药物 炎性细胞因子 肿瘤坏死因子 潜在作用 发斑 发病机制
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Influences of Annealing on the Opto—electronic Properties of ZnO Films Grown by Plasma—Enhanced MOCVD
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作者 王金忠 刘翔 《Chinese Physics Letters》 SCIE CAS CSCD 2002年第4期581-583,共3页
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Ultraviolet Lasing from ZnO Single Crystal at Room Temperature
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作者 向望华 孙懿 《Chinese Physics Letters》 SCIE CAS CSCD 2003年第2期296-297,共2页
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肾小管间质性肾炎抗原在肾脏研究中的进展 被引量:3
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作者 杨叶猗 肖力 +2 位作者 刘伏友 Yashpal S. Kanwar 孙林 《中华肾脏病杂志》 CAS CSCD 北大核心 2013年第5期396-399,共4页
肾小管间质性肾炎抗原(tubulointerstitialnephritisantigen,TINag)是一种主要表达在肾脏近曲小管基底膜,少部分表达在远端小管和鲍曼囊以及肠黏膜基底膜的膜糖蛋白,相对分子质量大致为48000—58000。TINag与Ⅳ型胶原蛋白和层粘连... 肾小管间质性肾炎抗原(tubulointerstitialnephritisantigen,TINag)是一种主要表达在肾脏近曲小管基底膜,少部分表达在远端小管和鲍曼囊以及肠黏膜基底膜的膜糖蛋白,相对分子质量大致为48000—58000。TINag与Ⅳ型胶原蛋白和层粘连蛋白等细胞外基质具有较高的亲和力,对维持小管基底膜的结构与功能具有重要作用。目前认为它也可能是人类抗肾小管基底膜(tubularbasementmembrane,TBM)抗体介导的问质性肾炎的特异性抗原。虽然TINag已明确主要表达在肾组织小管基底膜,有关其研究也已有近20年,但其生物学功能仍然不完全清楚。为此,我们结合国内外文献及我们以往的研究工作,综述TINag在肾脏病研究中的进展,以期引起同行重视。 展开更多
关键词 肾小管间质性肾炎 肾脏近曲小管 特异性抗原 肾小管基底膜 相对分子质量 Ⅳ型胶原蛋白 细胞外基质 层粘连蛋白
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GaAs衬底上分子束外延α-Sn薄膜及其量子阱结构考虑 被引量:1
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作者 易新建 郝建华 +1 位作者 张新宇 G.K.Wong 《中国科学(A辑)》 CSCD 1998年第2期166-170,共5页
利用分子束外延 (MBE)在GaAs( 0 0 1)单晶衬底上进行了α Sn薄膜的生长 ,利用反射高能电子显微镜 (RHEED) ,原位监控α Sn的生长过程和利用透射电子显微镜 (TEM )分析了界面结构 .测试结果表明 ,与以往报道相比亚稳态的α Sn膜具有高得... 利用分子束外延 (MBE)在GaAs( 0 0 1)单晶衬底上进行了α Sn薄膜的生长 ,利用反射高能电子显微镜 (RHEED) ,原位监控α Sn的生长过程和利用透射电子显微镜 (TEM )分析了界面结构 .测试结果表明 ,与以往报道相比亚稳态的α Sn膜具有高得多的温度稳定性 (由 70℃提高到 10 0℃ )和厚度稳定性 (由 5 0 0nm提高到 70 0nm) ,并观察到在其他电性能方面的改进 .此外 ,还提出了一种新型量子阱结构 . 展开更多
关键词 量子阱 量子尺寸效应 分子束外延 薄膜
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SHRIMP U-Pb zircon age of the Jinchuan ultramafic intrusion and its geological significance 被引量:40
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作者 LIXianhua SULi +1 位作者 SONGBiao LIUDunyi 《Chinese Science Bulletin》 SCIE EI CAS 2004年第4期420-422,共3页
关键词 锆石 超基性岩 侵入岩 地质学 片麻岩 大理石
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ON THE LIMIT CYCLES OF PLANAR AUTONOMOUS SYSTEMS 被引量:1
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作者 HANMAOAN QIANTIFEI 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2001年第3期297-306,共10页
The authors investigate the global properties of general autonomous systems on the plane and establish criteria for the nonexistence, existence and uniqueness of limit cycles. As application examples, the limit cycles... The authors investigate the global properties of general autonomous systems on the plane and establish criteria for the nonexistence, existence and uniqueness of limit cycles. As application examples, the limit cycles for some polynomial systems are studied. 展开更多
关键词 Limit cycle Planar autonomous system Existence.
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Frequency-dependent random fatigue of panel-type structures made of ceramic matrix composites 被引量:3
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作者 Yadong Zhou Xiaochen Hang +2 位作者 Shaoqing Wu Qingguo Fei Natasa Trisovic 《Acta Mechanica Solida Sinica》 SCIE EI CSCD 2017年第2期165-173,共9页
The panel-type structures used in aerospace engineering can be subjected to severe highfrequency acoustic loadings in service. This paper evaluates the frequency-dependent random fatigue of panel-type structures made ... The panel-type structures used in aerospace engineering can be subjected to severe highfrequency acoustic loadings in service. This paper evaluates the frequency-dependent random fatigue of panel-type structures made of ceramic matrix composites(CMCs) under acoustic loadings. Firstly, the high-frequency random responses from the broadband random excitation will result in more stress cycles in a deinite period of time. The probability density distributions of stress amplitudes will be different in different frequency bandwidths, though the peak stress estimations are identical. Secondly, the fatigue properties of CMCs can be highly frequency-dependent. The fatigue evaluation method for the random vibration case is adopted to evaluate the fatigue damage of a representative stiffened panel structure. The frequency effect through S-N curves on random fatigue damage is numerically veriied. Finally, a parameter is demonstrated to characterize the mean vibration frequency of a random process, and hence this parameter can further be considered as a reasonable loading frequency in the fatigue tests of CMCs to obtain more reliable S-N curves.Therefore, the inluence of vibration frequency can be incorporated in the random fatigue model from the two perspectives. 展开更多
关键词 Random fatigue Frequency effect Ceramic matrix composites(CMCs) S-N curve Loading frequency
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ENTROPIES AND FLUX-SPLITTINGS FOR THE ISENTROPIC EULER EQUATIONS
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作者 CHENGUIQIANG P.G.LEFLOCH 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2001年第2期145-158,共14页
The authors establish the existence of a large class of mathematical entropies (the so-called weak entropies) associated with the Euler equations for an isentropic, compressible fluid governed by a general pressure la... The authors establish the existence of a large class of mathematical entropies (the so-called weak entropies) associated with the Euler equations for an isentropic, compressible fluid governed by a general pressure law. A mild assumption on the behavior of the pressure law near the vacuum is solely required. The analysis is based on an asymptotic expansion of the fundamental solution (called here the entropy kernel) of a highly singular Euler- Poisson-Darboux equation. The entropy kernel is only Holder continuous and its regularity is carefully inversti- gated. Relying on a notion introduced earlier by the authors, it is also proven that, for the Euler equations, the set of eatropy flux-splittings coincides with the set of entropies-entropy fluxes. There results imply the existence of a flux-splitting consistent with all of the entropy inequalities. 展开更多
关键词 Compressible Euler equations Real gas hyperbolic Conservation law Entropy Euler- Poisson- Darboux equation
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DISCONTINUOUS SOLUTIONS IN L~∞ FOR HAMILTON-JACOBI EQUATIONS
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作者 CHENGUIQIANG SUBo 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2000年第2期165-186,共22页
An approach is introduced to construct global discontinuous solutions in L~∞ for HamiltonJacobi equations. This approach allows the initial data only in L~∞ and applies to the equations with nonconvex Hamiltonians.... An approach is introduced to construct global discontinuous solutions in L~∞ for HamiltonJacobi equations. This approach allows the initial data only in L~∞ and applies to the equations with nonconvex Hamiltonians. The profit functions are introduced to formulate the notion of discoatinuous solutions in L~∞. The existence of global discontinuous solutions in L~∞ is established. These solutions in L~∞ coincide with the viscosity solutions and the minimax solutions, provided that the initial data are continuous. A prototypical equation is analyzed to examine the L~∞ stability of our L~∞ solutions. The analysis also shows that global discontinuous solutions are determined by the topology in which the initial data are approximated. 展开更多
关键词 Hamilton-Jacobi equations Discontinuous solutions Profit functions Viscosity solutions Madman solutions STABILITY
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Uniqueness of Periodic Solutions for Certain Second-Order Equations
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作者 MaoAnHAN TiFeiQIAN 《Acta Mathematica Sinica,English Series》 SCIE CSCD 2004年第2期247-254,共8页
We establish a criterion for the uniqueness of periodic solutions for a class of second-order equations. We also give an application to a polynomial system and corrections to some known results.
关键词 Periodic solution UNIQUENESS Second-order system
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