Some experiments have shown that the properties of single mutation can be synthesizedin a mutant with multiple mutations. Following this idea, SRN-HI was prepared.Markussen et al.reported that a high stability and a p...Some experiments have shown that the properties of single mutation can be synthesizedin a mutant with multiple mutations. Following this idea, SRN-HI was prepared.Markussen et al.reported that a high stability and a prolonged action could be createdfrom mutations AsnA21Ser and ThrB27Arg respectively. Therefore, a double property wasexpected to appear in SRN-HI, which has been proved to be true by biological test.展开更多
Ⅰ. INTRODUCTIONThe chemical stability of human insulin preparations is directly related to the residue of A21-Asn at C-terminal of A chain and can be distinctly increased by substituting the A21 residue in the way of...Ⅰ. INTRODUCTIONThe chemical stability of human insulin preparations is directly related to the residue of A21-Asn at C-terminal of A chain and can be distinctly increased by substituting the A21 residue in the way of protein engineering. Studying the fine three-dimensional structures展开更多
It has long been found that insulin in acid solution is unstable. The main reason is that the A-chain C-terminal residue, asparagine, is very susceptible to acid hydrolysis, thereby a covalent-dimer formation takes pl...It has long been found that insulin in acid solution is unstable. The main reason is that the A-chain C-terminal residue, asparagine, is very susceptible to acid hydrolysis, thereby a covalent-dimer formation takes place readily due to the deamidation of this residue. As a number of pharmaceutical preparations of insulin are acidic solutions, this kind of instability has brought about a serious problem on purity and storage of this preparation in展开更多
基金Project supported by UNIDO grant (91/048) and "863" High-Tech Program of China (863-21-01).
文摘Some experiments have shown that the properties of single mutation can be synthesizedin a mutant with multiple mutations. Following this idea, SRN-HI was prepared.Markussen et al.reported that a high stability and a prolonged action could be createdfrom mutations AsnA21Ser and ThrB27Arg respectively. Therefore, a double property wasexpected to appear in SRN-HI, which has been proved to be true by biological test.
基金Project supported by the UNIDO(91/048/)and the "863" High-Tech Plan of China.
文摘Ⅰ. INTRODUCTIONThe chemical stability of human insulin preparations is directly related to the residue of A21-Asn at C-terminal of A chain and can be distinctly increased by substituting the A21 residue in the way of protein engineering. Studying the fine three-dimensional structures
基金Project supported by UNIDO(91/048)and High-Tech Program of China(103-19-01).
文摘It has long been found that insulin in acid solution is unstable. The main reason is that the A-chain C-terminal residue, asparagine, is very susceptible to acid hydrolysis, thereby a covalent-dimer formation takes place readily due to the deamidation of this residue. As a number of pharmaceutical preparations of insulin are acidic solutions, this kind of instability has brought about a serious problem on purity and storage of this preparation in
