The leaf extract of Mallotus japonicus and its major active constituent, rutin,suppressed on melanin production in murine B16F1 melanoma

Objective: To find anti-melanogenesis materials used in whitening cosmetics.Methods: The ethanolic leaf extract of Mallotus japonicus(M. japonicus) having an anti-melanogenesis activity was separated by a sephadex LH-20 chromatography. Each fraction was measured for its tyrosinase inhibitory activity together with its polyphenol content using the Folin–Ciocalteu method. The anti-melanogenesis activity of the active fractions was determined by the melanin content in the murine B16F1 melanoma. The active fractions were put together due to similar constituents, and then separated by high performance liquid chromatography using a C-18 ODS column. The major antimelanogenesis compound was identified using1 H and13C-NMR and liquid chromatography-mass spectrometry.Results: The ethanolic leaf extract of M. japonicus showed an anti-tyrosinase activity with a high polyphenol content, resulting in suppression of melanin production in the B16F1 melanoma. The extract was separated and the active compound was identical as rutin based on the1 H,13C-NMR and liquid chromatography-mass spectrometry analysis data. In addition, the rutin treatment with cells reduced the melanin content in a concentration dependent manner without any cell toxicity. The leaf extract of M. japonicus containing rutin would be useful in whitening cosmetics for protection from UV-light exposure to be limiting the accumulation of melanin in skin.Conclusions: The leaf extract of M. japonicus and/or rutin isolated from the extract as a key whitening agent would be useful as a whitening cosmetic material for protecting against disorder skin due to melanin accumulation.

Recent development of Faith conjecture

Is a semiprimary right self-injective ring a quasi-Frobenius ring？ Almost half century has passed since Faith raised this problem. He first conjectured “No” in his book Algebra II. Ring Theory in 1976, but changing his mind, he conjectured “Yes” in his article “When self-injective rings are QF： a report on a problem” in 1990. In this paper, we describe recent studies of this problem based on authors works and raise related problems.

Unsteady Correlation between Pressure and Temperature Field on Impinging Plate for Dual Underexpanded Jets

In this paper, dual underexpanded impinging jets are experimentally studied by measuring unsteady wall staticpressure and temperature fields using high response semiconductor pressure sensors and infrared imaging cam-era(100 captures per sec) with dual converging sonic nozzles. The flow field is also visualized by means of highspeed schlieren system( up to 70000 frame per second) to clarify the unsteady behavior of the jet structures suchas barrel shock wave, and Mach disk. Experiments were performed with a blow down tunnel changing the parameterssuch as the nozzle to plate distance, the distance between the two nozzles, and wind tunnel pressure ratio.The nozzle to plate distance L and the distance between the two nozzles H are non-dimensionalized by the nozzlediameter D covering H/D=1.5, 2.0, and 3, L/D= 2, 3,and 4, and po/pb=1.0 - 5.0, where po and pb are stagnationpressure and back pressure respectively. Although the response of the infrared camera is quite different fromthat of the high speed camera, the unsteady correlation between the pressure and temperature fields is confirmedunder some conditions. Also the high speed schlieren pictures and the wall static pressure fluctuations suggestthat the generation of the Mach disk promote the unsteady behavior of the jets. After the confirmation of the correlation,a simple way to find the severe fluctuating region can be provided according to the two dimensional unsteadytemperature images without a lot of unsteady pressure measurements.