NEW PARAMETER FOR DESCRIBING AND ANALYZING THE OPTICAL-ANISOTROPIC PROPERTIES OF BIOLOGICAL TISSUES

To characterize the degree of similarity inherent to parameters of the optically uniaxial birefringent protein-fibril networks of biological tissues,a new parameter-complex degree of mutual anisotropy-has been offered.The technique of polarization measuring the coordinate distributions of the complex degree of mutual anisotropy of biological tissues has been developed.It has been shown that statistical approach to the analysis of complex degree of mutual anisotropy distributions for biological tissues in various morphological and physiological states and for different optical thicknesses appears to be more sensitive and efficient in differentiation of physiological state,as compared to investigations of complex degree of mutual polarization in the corresponding laser images.

Multidither coherent optical adaptive technique for deep tissue two-photon microscopy

Two-photon microscopy normally suffers from the scattering of the tissue in biological imaging.Multidither coberent optical adaptive technique(COAT)can correct the scattered wavefront in parallel.However,the determination of the corrective phases may not be completely accurate using conventional method,which undermines the performance of this technique.In this paper,we theoretically demonstrate a method that can obtain more accurate corrective phases by determining the phase values from the square root of the fuorescence signal.A numnerical simulation model is established to study the performance of adaptive optics in two-photon micros-copy by combining scalar diffraction theory with vector diffraction theory.The results show that the distortion of the wavefront can be corrected more thoroughly with our method in two-photon imaging.In our simulation,with the scattering from a 450-mn-thick mouse brain tissue,excitation focal spots with higher peak-to background ratio(PBR)and images with higher contrast can be obtained.Hence,further enhancement of the multidither COAT correction performance in two-photon imaging can be expected.

CLASSIFYING OPTICAL PROPERTIES OF SURFACE-AND BULK-SCATTERING BIOLOGICAL LAYERS WITH POLARIZATION SINGULAR STATES

The results of singular approach usage in the tasks of description and classification of appearance of optical anisotropy of different types of phase-inhomogeneous biological layers(surface-scattering,optically thin and optically thick)have been presented.The characteristic values of the fourth Stokes vector parameter(S_(4)=0-linear polarization-(L-state);S_(4)=±1-circular polarization-(C-state))have been chosen as the main analytical tool descni bing polarization-singular states.The value of S.has been deternined by the value of phase shift betwoen the ort hogonal components of amplitude in the point of biological layer laser image and therefore is azimuthally stable.Hence,statistic moments of the first to the fourth orders characterizing the distribution of the amount of characteristic values S4=0;S_(4)=±1 have been used for definition and di ferentiation of optical properties of diferent types of biological layers-surface scattering,optically thin and optically thick human skin.

Laser polarization-variable autofluorescence of the network of optically anisotropic biological tissues:Diagnostics and differentiation of early stages of cancer of cervia uteri

This research presents the results of investigation of laser_polarization fluorescence ofbiological layers(histological sections,cy tological smears)in the task of diagnostics and differ-en tiation of early stages of cancer:Dysplasia-cervical microinvasive carcinoma of cervic uteri.The analytical conditions of polarization-optimal probing of biological layers were determinedbasing on the model of linear birefringence and dichroism of birefringent(fibrllar,porphyrin)networks.The technique of polarization-variable laser autofluorescence was developed andexperimentally tested.The objective criteria(statistical moments)of differentiation of histo-logical sections autofluorescent images of endometrium biopsy and cytological smears of itmucous coat were defined.The operational characteristics(sensitivity,speifity,accuracy)ofthis technique were determined concerning the positions of probative medicine,and clinical efficiency.

Optical transfer function analysis of circular-pupil wavefront coding systems with separable phase masks

This paper proposes a simple method to achieve the optical transfer function of a circular pupil wavefront coding system with a separable phase mask in Cartesian coordinates. Based on the stationary phase method, the optical transfer function of the circular pupil system can be easily obtained from the optical transfer function of the rectangular pupil system by modifying the cut-off frequency and the on-axial modulation transfer function. Finally, a system with a cubic phase mask is used as an example to illustrate the way to achieve the optical transfer function of the circular pupil system from the rectangular pupil system.

Propagation Effect of Hollow Gaussian Beams Passing through a Misaligned Optical System

A generalized formula of hollow Gaussian beams through the first-order misaligned ABCD systems is derived by using the generalized diffraction integral formula. It is shown that the hollow Gaussian beam passing through the misaligned system becomes a decentred hollow Gaussian beam. The propagation properties of the output beam are investigated when it propagates through a simple misaligned lens system. These results provide a powerful theoretical tool for applications of optical traps.

Propagation of Optical Pulses and Pulsed Beams in the Frequency Domain

The diffraction integral formulae in the temporal and spatial-temporal frequency domains are derived by using a Fourier transform and tensor analysis method. Based on these formulae, the abcd law in the temporal frequency domain and the tensor ABCD law in the four-dimensional spatial-temporal frequency domain are derived. An application example of the derived formulae is provided.

Atomic motion in the magneto–optical trap consisting of partially spatially coherent laser

Rb atom motion in a magneto–optical trap(MOT) consisting of a partially spatially coherent laser(PSCL) is investigated theoretically. The spatial coherence of the laser is controlled by the electro–optic crystal. The instantaneous spatial distribution of the dissipative force induced by the PSCL on an Rb atom is varying with time stochastically. The simulated results indicate that compared with a fully coherent laser, the spatial coherent laser has effects on the atomic trajectories;however, the capture velocity and the escape velocity are kept the same. The main reason is that the spatial coherence of the laser fluctuates temporally and spatially, but the average photon scattering rate varies little, which makes the total number of atoms and the atomic density distribution unchanged.

Simplifying the detection of optical distortions by machine learning

Adaptive optics has been widely used in biological science to recover high-resolution optical image deep into the tissue,where optical distortion detection with high speed and accuracy is strongly required.Here,we introduce convolutional neural networks,one of the most popular machine learning models,into Shack-Hartmann wavefront sensor(SHWS)to simplify optical distortion detection processes.Without image segmentation or centroid positioning algorithm,the trained network could estimate up to 36th Zernike mode coefficients directly from a full SHWS image within 1.227ms on a personal computer,and achieves prediction accuracy up to 97.4%.The simulation results show that the average root mean squared error in phase residuals of our method is 75.64%lower than that with the modal-based SHWS method.With the high detection accuracy and simplified detection processes,this work has the potential to be applied in wavefront sensor-based adaptive optics for in vivo deep tissue imaging.

Holograms in Albumins and Optical Properties Recorded in Real Time

Holographic recording analysis was performed on the films that were sensitized with ammonium dichromate with albumin of hen and quail (Gallus gallus and Callipepla cali) as abiopolymeric photosensitive matrix. The samples were exposed to an He-Cd laser, λ = 442 nm, at various concentrations, and diffraction efficiencies were measured as a function to the energy, thickness, protein density, aging time, and spatial frequencies. The photosensitivity was measured as a function of storage time, and the gratings were recorded in real time. The photochemical processes involved in the formation of holographic image are described. The results indicate the behavior of colloidal systems based on albumin bird build holographic recording materials.

Experimental Investigation on a Highly Sensitive Atomic Magnetometer

A highly sensitive all-optical atomic magnetometer based on the magnetooptical effect which uses the advanced technique of single laser beam detection is reported and demonstrated experimentally. A sensitivity of 0.5pT/Hz^1/2 is obtained by analyzing the magnetic noise spectrum, which exceeds that of most traditional magnetometers. This kind of atomic magnetometer is very compact, has a low power consumption, and has a high theoretical sensitivity limit, which make it suitable for many applications.

A Compact Setup of Saturated Absorption Spectroscopy for Diode Laser Frequency Stabilization

A novel setup of saturated absorption spectroscopy (SAS) is presented.It is based on laser reflections at surfaces of a sample vapor cell.It only needs one cell and one photodiode and is more compact than conventional setups of SAS.Its spectrum is similar to a conventional SAS.The frequency stabilization performance of an external-cavity diode laser with this setup is investigated.A frequency stability of 1.1 × 10^(-11) is achieved at an averaging time of 60s in the Allen variance measurements.

Laser frequency stabilization and shifting by using modulation transfer spectroscopy

The stabilizing and shifting of laser frequency are very important for the interaction between the laser and atoms. The modulation transfer spectroscopy for the 87Rb atom with D2 line transition F = 2 →F＇ = 3 is used for stabilizing and shifting the frequency of the external cavity grating feedback diode laser. The resonant phase modulator with electro-optical effect is used to generate frequency sideband to lock the laser frequency. In the locking scheme, circularly polarized pump- and probe-beams are used. By optimizing the temperature of the vapor, the pump- and probe-beam intensity, the laser linewidth of 280 kHz is obtained. Furthermore, the magnetic field generated by a solenoid is added into the system. Therefore the system can achieve the frequency locking at any point in a range of hundreds of megahertz frequency shifting with very low power loss.

Fabrication and characterization of rugate structures composed of SiO_2 and Nb_2O_5

Gradient index layers and rugate structures were fabricated on a Leybold Syrus pro deposition system by plasma-assisted coevaporation of the low index material silica and the high index material niobium pentoxide. To obtain information about the compositional profiles of the produced layers, cross sectional transmission electron microscopy was used in assistance to deposition rate data recorded by two independent crystal monitors during the film preparation. The depth dependent concentration profiles were transformed to refractive index gradients by means of effective medium approximation. Based on the refractive index gradients the corresponding samples` transmission and reflection spectra could be calculated by utilizing matrix formalism. The relevance of the established refractive index profiles could be verified by comparison of the calculated spectra with the measured ones.

Simple PSF based method for pupil phase mask’s optimization in wavefront coding system

By applying the wavefront coding technique to an optical system, the depth of focus can be greatly increased. Several complicated methods, such as Fisher Information based method, have already been taken to optimize for the best pupil phase mask in ideal condition. Here one simple point spread function (PSF) based method with only the standard deviation method used to evaluate the PSF stability over the depth of focus is taken to optimize for the best coefficients of pupil phase mask in practical optical systems. Results of imaging simulations for optical systems with and without pupil phase mask are presented, and the sharpness of image is calculated for comparison. The optimized results showed better and much more stable imaging quality over the original system without changing the position of the image plane.

High contrast atomic magnetometer based on coherent population trapping

We present an experimental and theoretical investigation of the coherent population trapping （CPT） resonance excited on the D1 line of 87Rb atoms by bichromatic linearly polarized laser light. The experimental results show that a lin｜｜lin tran- sition scheme is a promising alternative to the conventional circular-circular transition scheme for an atomic magnetometer. Compared with the circular light transition scheme, linear light accounts for high-contrast transmission resonances, which makes this excitation scheme promising for high-sensitivity magnetometers. We also use linear light and circular light to detect changes of a standard magnetic field, separately.

Quantum state transfer between atomic ensembles trapped in separate cavities via adiabatic passage

We propose a new approach for quantum state transfer（QST） between atomic ensembles separately trapped in two distant cavities connected by an optical fiber via adiabatic passage. The three-level Λ-type atoms in each ensemble dispersively interact with the nonresonant classical field and cavity mode. By choosing appropriate parameters of the system, the effective Hamiltonian describes two atomic ensembles interacting with ＂the same cavity mode＂ and has a dark state. Consequently, the QST between atomic ensembles can be implemented via adiabatic passage. Numerical calculations show that the scheme is robust against moderate fluctuations of the experimental parameters. In addition, the effect of decoherence can be suppressed effectively. The idea provides a scalable way to an atomic-ensemble-based quantum network, which may be reachable with currently available technology.

Objective assessment of the effect of pupil size upon the power distribution of multifocal contact lenses

AIM： To analytically assess the effect of pupil size upon the refractive power distributions of different designs of multifocal contact lenses.METHODS： Two multifocal contact lenses of center-near design and one multifocal contact lens of center-distance design were used in this study. Their power profiles were measured using the NIMO TR1504 device （LAMBDA-X, Belgium）. Based on their power profiles, the power distribution was assessed as a function of pupil size. For the high addition lenses, the resulting refractive power as a function of viewing distance （far, intermediate, and near） and pupil size was also analyzed.RESULTS： The power distribution of the lenses was affected by pupil size differently. One of the lenses showed a significant spread in refractive power distribution, from about ？3 D to 0 D. Generally, the power distribution of the lenses expanded as the pupil diameter became greater. The surface of the lens dedicated for each distance varied substantially with the design of the lens.CONCLUSION： In an experimental basis, our results show how the lenses power distribution is affected by the pupil size and underlined the necessity of careful evaluation of the patient’s visual needs and the optical properties of a multifocal contact lens for achieving the optimal visual outcome.

Investigation of the thermal adaptability for a mobile cold atom gravimeter

The cold atom gravimeter offers the prospect of a new generation of inertial sensors for field applications. We ac- complish a mobile atom gravimeter. With the compact and stable system, a sensitivity of 1.4× 10-7 g.Hz-1/2 is achieved. Moreover, a continuous gravity monitoring of 80 h is carried out. However, the harsh outdoor environment is a big challenge for the atom gravimeter when it is for field applications. In this paper, we present the preliminary investigation of the thermal adaptability for our mobile cold atom gravimeter. Here, we focus on the influence of the air temperature on the performance of the atom gravimeter. The responses to different factors （such as laser power, fiber coupling efficiency, etc.） are evaluated when there is a great temperature shift of 10 ℃. The result is that the performances of all the factors deteriorate to different extent, nevertheless, they can easily recover as the temperature comes back. Finally, we conclude that the variation of air temperature induces the increase of noise and the system error of the atom gravimeter as well, while the process is reversible with the recovery of the temperature.

A laser pump-re-pump atomic magnetometer

We demonstrate experimentally an atomic magnetometer based on optical pumping theory, a magnetic resonance that is induced by a radio frequency field and dependent on the magnetic field strength. Compared with the conventional method using one radiation field, which is used not only as the probe beam but also as a pump beam, the additional re-pump beam can increase remarkably the amplitude of the signal. It is shown that the amplitude of the magnetic field resonance signal can increase more than 55% by using an additional re-pump beam, which makes the sensitivity of the magnetometer higher. Finally, we investigate the relation between amplitude of the signal and re-pump laser power, and calculate the atomic population in the trapping states with rate equations.