With reported pathogenic genes of Botrytis cirterea Pers. and unknown proteins related to pathogencity as experimental materials, the pathogenic genes were studied by bioinformatics methods, and these unknown proteins...With reported pathogenic genes of Botrytis cirterea Pers. and unknown proteins related to pathogencity as experimental materials, the pathogenic genes were studied by bioinformatics methods, and these unknown proteins were analyzed with bioinforrnaties tools and softwares, so as to lay a theoretical basis for the control of tomato grey mould at molecular level in future. The results showed that through the alignment analysis by DNAMAN software, three pairs of unknown pro- teins with higher comprehensive matching degrees were obtained, and these proteins were predicted to be coded by hrp gene cluster of type Ili secretion system; through Bioedit and ProtParam, the amino acid composition, size, molecular weight, isoelectrie point and hydrophobicity of the each of the six unknown proteins were obtained; and corresponding secondary structures were obtained using DNAMAN. The results of this study could guide deep research of such genes.展开更多
[Objectives] The research aimed to investigate the genetic rule of the disease resistance of tomato CLN2037,find molecular markers linked with disease-resistant genes and locate the disease-resistant genes. [Methods] ...[Objectives] The research aimed to investigate the genetic rule of the disease resistance of tomato CLN2037,find molecular markers linked with disease-resistant genes and locate the disease-resistant genes. [Methods] BCr,BCs and F2 were constructed from tomato CLN2037 and susceptible cultivar T2-03,and their resistance to late blight was identified and analyzed by inoculating the disease in the seedling stage of tomatoes. The genetic linkage analysis was made based on the genetic linkage map constructed by ICu GI using group segregation method and 90 pairs of tomato SSR primers. [Results]The resistance of CLN2037 to late blight was controlled by a single recessive gene. The polymorphic bands amplified by primer DM0231 had a linkage relationship with resistance genes. The size of the polymorphism fragment was 226 bp with the genetic linkage distance of 2. 67 c M,and the disease resistance gene was localized on CCPB272-03741.[Conclusions]DM0231 could be used as a molecular marker for molecular breeding of tomato against late blight,which laid the foundation for further fine localization of late blight resistance genes.展开更多
基金Supported by Science and Technology Pilot Project of Xinjiang Agricultural Reclamation Academy of Science(50YYD201610)
文摘With reported pathogenic genes of Botrytis cirterea Pers. and unknown proteins related to pathogencity as experimental materials, the pathogenic genes were studied by bioinformatics methods, and these unknown proteins were analyzed with bioinforrnaties tools and softwares, so as to lay a theoretical basis for the control of tomato grey mould at molecular level in future. The results showed that through the alignment analysis by DNAMAN software, three pairs of unknown pro- teins with higher comprehensive matching degrees were obtained, and these proteins were predicted to be coded by hrp gene cluster of type Ili secretion system; through Bioedit and ProtParam, the amino acid composition, size, molecular weight, isoelectrie point and hydrophobicity of the each of the six unknown proteins were obtained; and corresponding secondary structures were obtained using DNAMAN. The results of this study could guide deep research of such genes.
基金Supported by the Science and Technology Pilot Project of Xinjiang Academy of Agricultural Reclamation Science(50YYD201610)
文摘[Objectives] The research aimed to investigate the genetic rule of the disease resistance of tomato CLN2037,find molecular markers linked with disease-resistant genes and locate the disease-resistant genes. [Methods] BCr,BCs and F2 were constructed from tomato CLN2037 and susceptible cultivar T2-03,and their resistance to late blight was identified and analyzed by inoculating the disease in the seedling stage of tomatoes. The genetic linkage analysis was made based on the genetic linkage map constructed by ICu GI using group segregation method and 90 pairs of tomato SSR primers. [Results]The resistance of CLN2037 to late blight was controlled by a single recessive gene. The polymorphic bands amplified by primer DM0231 had a linkage relationship with resistance genes. The size of the polymorphism fragment was 226 bp with the genetic linkage distance of 2. 67 c M,and the disease resistance gene was localized on CCPB272-03741.[Conclusions]DM0231 could be used as a molecular marker for molecular breeding of tomato against late blight,which laid the foundation for further fine localization of late blight resistance genes.
