Osteosarcoma is the most common primary malignant neoplasm of the bone in children and adolescents and has a high risk of relapse and metastasis. Of the various methods to treat osteosarcoma, the use of genetic approa...Osteosarcoma is the most common primary malignant neoplasm of the bone in children and adolescents and has a high risk of relapse and metastasis. Of the various methods to treat osteosarcoma, the use of genetic approaches to inhibit the rapid growth of osteosarcoma while limiting tumor metastasis has presented a challenge in its implementation. Here, we successfully synthesized a polysaccharide derivative （Amy-g-PLLD） for delivery of astrocyte elevated gene-1 （AEG-1） small-interfering RNA （siRNA） （siAEG-1）, and used it for the first time to suppress osteosarcoma tumors in vitro and in vivo. Amy-g-PLLD/ siAEG-1 complexes were delivered into 143B human osteosarcoma cells with low resultant cytotoxicity. Osteosarcoma tumor proliferation and invasion were inhibited in vitro. Intratumoral injection of Amy-g-PLLD/siAEG-1 complexes markedly inhibited tumor growth and lung metastasis in 143B tumor-bearing mice. This biocompatible and effective approach employing a natural material- siRNA complex should pave the way for more genetic research in treating osteosarcoma.展开更多
Yolk-shell nanostructures have recently attracted tremendous research interests in various areas because of their unique structural merits.Currently,there is an urgent need for developing porous shells with multifunct...Yolk-shell nanostructures have recently attracted tremendous research interests in various areas because of their unique structural merits.Currently,there is an urgent need for developing porous shells with multifunctional features to enhance their performance in various applications.Herein,advanced yolk-shell nanospheres have been facilely prepared by encapsulating silver nanoparticles with porous Janus polymer shells that consist of a hypercrosslinked polystyrene(xPS)outer layer and a tethered poly(acrylic acid)(PAA)brush lining.The xPS outer layer possesses well-developed porosity as mass diffusion pathways.More importantly,the tethered PAA brushes with customized carboxyl groups exhibit great affinity toward specific species(e.g.,cationic dyes),leading to their enrichment from the bulk solution into the interior cavity.The unusual combination of highly porous outer layers with customizable inbuilt polymer linings in the porous Janus shells endows them with great promise for synergistically catalytic degradation of cationic dyes.展开更多
基金This work was supported by the National Natural Science Foundation of China （Nos. 81402221 and 51273216）, the Research Fund for the Doctoral Program of Higher Education of China （No. 20130171120077）, the Science and Technology Program of Guangzhou, China （No. 201707010108）, the Guangdong Innovative Research Team Program （No. 2009010057）, and the Science and Technology Planning Project of Guangzhou （No. 201610010006）, the Science and Technology Planning project of Guangdong Province （No. 20153900042020319） and Natural Science Foundation of Guangdong Province （No. 2016A030313819）.
文摘Osteosarcoma is the most common primary malignant neoplasm of the bone in children and adolescents and has a high risk of relapse and metastasis. Of the various methods to treat osteosarcoma, the use of genetic approaches to inhibit the rapid growth of osteosarcoma while limiting tumor metastasis has presented a challenge in its implementation. Here, we successfully synthesized a polysaccharide derivative （Amy-g-PLLD） for delivery of astrocyte elevated gene-1 （AEG-1） small-interfering RNA （siRNA） （siAEG-1）, and used it for the first time to suppress osteosarcoma tumors in vitro and in vivo. Amy-g-PLLD/ siAEG-1 complexes were delivered into 143B human osteosarcoma cells with low resultant cytotoxicity. Osteosarcoma tumor proliferation and invasion were inhibited in vitro. Intratumoral injection of Amy-g-PLLD/siAEG-1 complexes markedly inhibited tumor growth and lung metastasis in 143B tumor-bearing mice. This biocompatible and effective approach employing a natural material- siRNA complex should pave the way for more genetic research in treating osteosarcoma.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.51925308,U1601206,51672313,51702370,51872336)National Program for Support of Top-notch Young Professionals,Leading Scientific,Technical and Innovation Talents of Guangdong Special Support Program(No.2017TX04C248)+1 种基金Fundamental Research Funds for the Central Universities(Nos.18lgzd10,17lgpy83)the Science and Technology Program of Guangzhou(No.201704020059).
文摘Yolk-shell nanostructures have recently attracted tremendous research interests in various areas because of their unique structural merits.Currently,there is an urgent need for developing porous shells with multifunctional features to enhance their performance in various applications.Herein,advanced yolk-shell nanospheres have been facilely prepared by encapsulating silver nanoparticles with porous Janus polymer shells that consist of a hypercrosslinked polystyrene(xPS)outer layer and a tethered poly(acrylic acid)(PAA)brush lining.The xPS outer layer possesses well-developed porosity as mass diffusion pathways.More importantly,the tethered PAA brushes with customized carboxyl groups exhibit great affinity toward specific species(e.g.,cationic dyes),leading to their enrichment from the bulk solution into the interior cavity.The unusual combination of highly porous outer layers with customizable inbuilt polymer linings in the porous Janus shells endows them with great promise for synergistically catalytic degradation of cationic dyes.
