Purpose An important outcome measure of patient care is the impact on the patient’s health-related quality of life(HRQoL).Current ear-specific HRQoL instruments are designed for one diagnosis and emphasize different ...Purpose An important outcome measure of patient care is the impact on the patient’s health-related quality of life(HRQoL).Current ear-specific HRQoL instruments are designed for one diagnosis and emphasize different subdivisions such as symptoms,hearing problems,psychosocial impact,and the need for care.The optimal length of the recall period has not been studied.For these reasons,a new survey is needed that would cover most chronic ear diseases.Methods A preliminary 24-item survey(EOS-24)was created.Untreated adult patients(included n=186)with one of seven different chronic otologic conditions from all university hospitals in Finland were recruited to respond to EOS-24 and the 15D general HRQoL instrument.The recruiting otologists evaluated the severity of the disease and the disability caused by it.A control group was recruited.Based on the patients’responses in different diagnosis groups,the items were reduced according to pre-defined criteria.The resulting survey was validated using a thorough statistical analysis.Results The relevance and necessity of the original 24 items were thoroughly investigated,leading to the exclusion of 8 items and the modification of 1.The remaining 16 items were well-balanced between subdivisions and were useful in all seven diagnosis groups,thus constituting the final instrument,EOS-16.The most suitable recall period was three months.Conclusions EOS-16 has been created according to the HRQoL survey guidelines with a versatile nationwide patient population.The survey has been validated and can be used for a wide range of chronic ear diseases as a HRQoL instrument.展开更多
This study aims to elucidate the nature of cognitive deficits caused by intracranial tumors, as well as to examine how a surgical operation of the tumor may affect tumor-induced cognitive deficits. The patient group i...This study aims to elucidate the nature of cognitive deficits caused by intracranial tumors, as well as to examine how a surgical operation of the tumor may affect tumor-induced cognitive deficits. The patient group included 43 individuals with meningioma or low-grade glioma admitted to a surgical operation of the tumor. Neuropsychological examination was conducted preoperatively, as well as three and 12 months postoperatively. The control group comprised 31 healthy subjects. In the tumor patients, preoperative cognitive performance was compromised in several cognitive domains as compared to the controls. The tumor patients with frontal and large tumors showed impairment virtually across all cognitive domains. Postoperatively, the cognitive performance of the meningioma and the small tumor group improved in all domains, with the performance of the low-grade glioma group and the large tumor group reflecting more modest cognitive improvement. Most of this improvement did not emerge until the 12 months follow-up. Cognitive impairment due to an intracranial tumor is diffuse affecting most cognitive domains. Cognitive recovery after the surgery is more noticeable in patients with meningiomas and small tumors, and the recovery will require a minimum of one year time-wise. This evidence is of significant value when planning both clinical treatment and rehabilitation of intracranial tumor patients.展开更多
Background:Transcriptomic and proteomic profiling of human brain tissue is hindered by the availability of fresh samples from living patients.Postmortem samples usually represent the advanced disease stage of the pati...Background:Transcriptomic and proteomic profiling of human brain tissue is hindered by the availability of fresh samples from living patients.Postmortem samples usually represent the advanced disease stage of the patient.Fur-thermore,the postmortem interval can affect the transcriptomic and proteomic profiles.Therefore,fresh brain tissue samples from living patients represent a valuable resource of metabolically intact tissue.Implantation of deep brain stimulation(DBS)electrodes into the human brain is a neurosurgical treatment for,e.g.,movement disorders.Here,we describe an improved approach to collecting brain tissues from surgical instruments used in implantation of DBS device for transcriptomics and proteomics analyses.Methods:Samples were extracted from guide tubes and recording electrodes used in routine DBS implantation procedure to treat patients with Parkinson’s disease,genetic dystonia and tremor.RNA sequencing was performed in tissues extracted from the recording microelectrodes and liquid chromatography-mass spectrometry(LC-MS)per-formed in tissues from guide tubes.To assess the performance of the current approach,the obtained datasets were compared with previously published datasets representing brain tissues.Results:Altogether,32,034 RNA transcripts representing the unique Ensembl gene identifiers were detected from eight samples representing both hemispheres of four patients.By using LC-MS,we identified 734 unique proteins from 31 samples collected from 14 patients.The datasets are available in the BioStudies database(accession number S-BSST667).Our results indicate that surgical instruments used in DBS installation retain brain material sufficient for protein and gene expression studies.Comparison with previously published datasets obtained with similar approach proved the robustness and reproducibility of the protocol.Conclusions:The instruments used during routine DBS surgery are a useful source for obtaining fresh brain tis-sues from living patients.This approach overcomes the issues that arise from using postmortem tissues,such as the effect of postmortem interval on transcriptomic and proteomic landscape of the brain,and can be used for studying molecular aspects of DBS-treatable diseases.展开更多
文摘Purpose An important outcome measure of patient care is the impact on the patient’s health-related quality of life(HRQoL).Current ear-specific HRQoL instruments are designed for one diagnosis and emphasize different subdivisions such as symptoms,hearing problems,psychosocial impact,and the need for care.The optimal length of the recall period has not been studied.For these reasons,a new survey is needed that would cover most chronic ear diseases.Methods A preliminary 24-item survey(EOS-24)was created.Untreated adult patients(included n=186)with one of seven different chronic otologic conditions from all university hospitals in Finland were recruited to respond to EOS-24 and the 15D general HRQoL instrument.The recruiting otologists evaluated the severity of the disease and the disability caused by it.A control group was recruited.Based on the patients’responses in different diagnosis groups,the items were reduced according to pre-defined criteria.The resulting survey was validated using a thorough statistical analysis.Results The relevance and necessity of the original 24 items were thoroughly investigated,leading to the exclusion of 8 items and the modification of 1.The remaining 16 items were well-balanced between subdivisions and were useful in all seven diagnosis groups,thus constituting the final instrument,EOS-16.The most suitable recall period was three months.Conclusions EOS-16 has been created according to the HRQoL survey guidelines with a versatile nationwide patient population.The survey has been validated and can be used for a wide range of chronic ear diseases as a HRQoL instrument.
文摘This study aims to elucidate the nature of cognitive deficits caused by intracranial tumors, as well as to examine how a surgical operation of the tumor may affect tumor-induced cognitive deficits. The patient group included 43 individuals with meningioma or low-grade glioma admitted to a surgical operation of the tumor. Neuropsychological examination was conducted preoperatively, as well as three and 12 months postoperatively. The control group comprised 31 healthy subjects. In the tumor patients, preoperative cognitive performance was compromised in several cognitive domains as compared to the controls. The tumor patients with frontal and large tumors showed impairment virtually across all cognitive domains. Postoperatively, the cognitive performance of the meningioma and the small tumor group improved in all domains, with the performance of the low-grade glioma group and the large tumor group reflecting more modest cognitive improvement. Most of this improvement did not emerge until the 12 months follow-up. Cognitive impairment due to an intracranial tumor is diffuse affecting most cognitive domains. Cognitive recovery after the surgery is more noticeable in patients with meningiomas and small tumors, and the recovery will require a minimum of one year time-wise. This evidence is of significant value when planning both clinical treatment and rehabilitation of intracranial tumor patients.
基金the Academy of Finland(Decision Numbers#311934 R.H.[profiling programme]and#331436 J.U.),Pediatric Research Foundation,Finland(J.U.and R.H.),Biocenter Oulu(J.U.and R.H.),Biocenter Finland,Special State Grants for Health Research,Oulu University Hospital,Finland(J.U.)the Terttu Foundation,Oulu University Hospital,Finland(J.K.).
文摘Background:Transcriptomic and proteomic profiling of human brain tissue is hindered by the availability of fresh samples from living patients.Postmortem samples usually represent the advanced disease stage of the patient.Fur-thermore,the postmortem interval can affect the transcriptomic and proteomic profiles.Therefore,fresh brain tissue samples from living patients represent a valuable resource of metabolically intact tissue.Implantation of deep brain stimulation(DBS)electrodes into the human brain is a neurosurgical treatment for,e.g.,movement disorders.Here,we describe an improved approach to collecting brain tissues from surgical instruments used in implantation of DBS device for transcriptomics and proteomics analyses.Methods:Samples were extracted from guide tubes and recording electrodes used in routine DBS implantation procedure to treat patients with Parkinson’s disease,genetic dystonia and tremor.RNA sequencing was performed in tissues extracted from the recording microelectrodes and liquid chromatography-mass spectrometry(LC-MS)per-formed in tissues from guide tubes.To assess the performance of the current approach,the obtained datasets were compared with previously published datasets representing brain tissues.Results:Altogether,32,034 RNA transcripts representing the unique Ensembl gene identifiers were detected from eight samples representing both hemispheres of four patients.By using LC-MS,we identified 734 unique proteins from 31 samples collected from 14 patients.The datasets are available in the BioStudies database(accession number S-BSST667).Our results indicate that surgical instruments used in DBS installation retain brain material sufficient for protein and gene expression studies.Comparison with previously published datasets obtained with similar approach proved the robustness and reproducibility of the protocol.Conclusions:The instruments used during routine DBS surgery are a useful source for obtaining fresh brain tis-sues from living patients.This approach overcomes the issues that arise from using postmortem tissues,such as the effect of postmortem interval on transcriptomic and proteomic landscape of the brain,and can be used for studying molecular aspects of DBS-treatable diseases.
