A simplified LC-MS/MS method for the quantification of the cardiovascular disease biomarker trimethylamine-N-oxide and its precursors

Trimethylamine-N-oxide(TMAO)has emerged as a potential biomarker for atherosclerosis and the development of cardiovascular diseases(CVDs).Although several clinical studies have shown striking associations of TMAO levels with atherosclerosis and CVDs,TMAO determinations are not clinical routine yet.The current methodology relies on isotope-labeled internal standards,which adds to pre-analytical complexity and costs for the quantification of TMAO and its precursors carnitine,betaine or choline.Here,we report a liquid chromatography-tandem mass spectrometry based method that is fast(throughput up to 240 samples/day),consumes low sample volumes(e.g.,from a finger prick),and does not require isotope-labeled standards.We circumvented the analytical problem posed by the presence of endogenous TMAO and its precursors in human plasma by using an artificial plasma matrix for calibration.We cross-validated the results obtained using an artificial matrix with those using mouse plasma matrix and demonstrated that TMAO,carnitine,betaine and choline were accurately quantified in’reallife’human plasma samples from healthy volunteers,obtained either from a finger prick or from venous puncture.Additionally,we assessed the stability of samples stored at-20℃and room temperature.Whereas all metabolites were stable at-20℃,increasing concentrations of choline were determined when stored at room temperature.Our method will facilitate the establishment of TMAO as a routine clinical biomarker in hematology in order to assess the risk for CVDs development,or to monitor disease progression and intervention effects.

Immunological aspects of liver cell transplantation

Within the field of regenerative medicine, the liver is of major interest for adoption of regenerative strategies due to its well-known and unique regenerative capacity. Whereas therapeutic strategies such as liver resection and orthotopic liver transplantation(OLT) can be considered standards of care for the treatment of a variety of liver diseases, the concept of liver cell transplantation(LCTx) still awaits clinical breakthrough. Success of LCTx is hampered by insufficient engraftment/long-term acceptance of cellular allografts mainly due to rejection of transplanted cells. This is in contrast to the results achieved for OLT where longterm graft survival is observed on a regular basis and, hence, the liver has been deemed an immuneprivileged organ. Immune responses induced by isolated hepatocytes apparently differ considerably from those observed following transplantation of solid organs and, thus, LCTx requires refined immunological strategies to improve its clinical outcome. In addition, clinical usage of LCTx but also related basic research efforts are hindered by the limited availability of high quality liver cells, strongly emphasizing the need for alternative cell sources. This review focuses on the various immunological aspects of LCTx summarizing data available not only for hepatocyte transplantation but also for transplantation of non-parenchymal liver cells and liver stem cells.

Profound neutralization evasion and augmented host cell entry are hallmarks of the fast-spreading SARS-CoV-2 lineage XBB.1.5

Since late 2022, the share of infections caused by the SARS-CoV2 lineage XBB.1.5 has gradually increased in the United States,resulting in XBB.1.5 becoming the dominating SARS-CoV-2lineage in the United States and a similar trend is likely to soontake place also in European countries. However, information onthe virological properties of XBB.1.5 is scarce. Here, weconducted an initial virological assessment of the SARS-CoV-2XBB.1.5 lineage.

Host cell entry and neutralisation sensitivity of the SARS-CoV-2 XBB.1.16 lineage

Despite previous circulation of the highly transmissible and antibody evasive BA.2.75, BQ.1, XBB.1 and XBB.1.5 lineages, the share of infections caused by the SARS-CoV-2 lineage XBB.1.16 has gradually increased in India in early 2023, resulting in XBB.1.16 being the dominating SARS-CoV-2 lineage in India today. Since a similar trend may also take place in other countries and information on the biological properties of the XBB.1.16 lineage is scarce, we conducted a rapid assessment of the SARS-CoV-2 XBB.1.16 lineage with respect to its ability to enter cells and evade neutralisation by antibodies.

Phylogenetic and chemotaxonomic resolution of the genus Annulohypoxylon(Xylariaceae)including four new species

This study deals with an extensive evaluation focusing on phylogenetic and chemotaxonomic infrageneric relationships of the genus Annulohypoxylon(Xylariaceae,Ascomycota),whose species are ubiquitously associated with seed plants as endophytes and saprotrophs in all forested areas of the world.Using evidence from phylogenetic,chemotaxonomic and morphological data,various varieties within the genus are raised to species level,leading to the new combinations for A.areolatum(:A.bovei var.microsporum),A.macrosporum(:A.leptascum var.macrosporum),and A.microdiscum(:A.moriforme var.microdiscum).Annulohypoxylon substygium nom.nov.is applied for A.stygium var.annulatumand the four new tropical and subtropical species A.massivum,A.violaceopigmentum,A.viridistratum and A.yungensis are introduced.Furthermore,A.leucadendri is excluded from the genus as its morphological characters disagree with the generic concept,the recently erected A.palmicola is synonymized with A.leptascum and A.austrobahiense has been reassigned to the genus Hypoxylon.In addition,the key taxa A.annulatum and A.truncatum have been reinvestigated and epitypes have been defined.For the first time,a dichotomous key to the genus is provided.A phylogenetic b-tubulin tree in conjunction with stromatal HPLC profiles clearly shows that Annulohypoxylon comprises two distinct lineages.The A.cohaerens/multiforme group might eventually warrant segregation into a new genus as further molecular data become available.

Lenormandins A—G,new azaphilones from Hypoxylon lenormandii and Hypoxylon jaklitschii sp.nov.,recognised by chemotaxonomic data

Seven new azaphilone pigments named lenormandins A–G were isolated from stromata(fruiting bodies)of the xylariaceous fungus,Hypoxylon lenormandii using preparative High Performance Liquid Chromatography(HPLC)and their structures were elucidated by NMR spectroscopy,high resolution mass spectrometry and other spectral methods.Their occurrence in herbarium specimens(including various type materials collected in the 19th and early 20th century)and several fresh collections was studied by analytical HPLC with diode array and mass spectrometric detection(HPLC-DAD/MS),revealing that this group of pigments is specific for Hypoxylon lenormandii from various geographic regions and otherwise only occurs in closely related taxa,i.e.H.sublenormandii and an undescribed fungus from Sri Lanka,for which we propose the name H.jaklitschii.The status of the new species was established by using molecular phylogenetic data based on ITS and partial beta-tubulin sequences,and by detailed morphological studies.

Thailand’s amazing diversity:up to 96%of fungi in northern Thailand may be novel

Fungi have been often neglected,despite the fact that they provided penicillin,lovastatin and many other important medicines.They are an understudied,but essential,fascinating and biotechnologically useful group of organisms.The study of fungi in northern Thailand has been carried out by us since 2005.These studies have been diverse,ranging from ecological aspects,phylogenetics with the incorportation of molecular dating,taxonomy(including morphology and chemotaxonomy)among a myriad of microfungi,to growing novel mushrooms,and DNA-based identification of plant pathogens.In this paper,advances in understanding the biodiversity of fungi in the region are discussed and compared with those further afield.Many new species have been inventoried for the region,but many unknown species remain to be described and/or catalogued.For example,in the edible genus Agaricus,over 35 new species have been introduced from northern Thailand,and numerous other taxa await description.In this relatively well known genus,93%of species novelty is apparent.In the microfungi,which are relatively poorly studied,the percentage of novel species is,surprisingly,generally not as high(55–96%).As well as Thai fungi,fungi on several hosts from Europe have been also investigated.Even with the well studied European microfungi an astounding percentage of new taxa(32–76%)have been discovered.The work is just a beginning and it will be a daunting task to document this astonishingly high apparent novelty among fungi.

Low serum neutralizing anti-SARS-CoV-2 S antibody levels in mildly affected COVID-19 convalescent patients revealed by two different detection methods

Neutralizing antibodies targeting the receptor-binding domain(RBD)of the SARS-CoV-2 spike(S)block severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)entry into cells via surface-expressed angiotensin-converting enzyme 2(ACE2).We used a surrogate virus neutralization test(sVNT)and SARS-CoV-2 S protein-pseudotyped vesicular stomatitis virus(VSV)vector-based neutralization assay(pVNT)to assess the degree to which serum antibodies from coronavirus disease 2019(COVID-19)convalescent patients interfere with the binding of SARS-CoV-2 S to ACE2.Both tests revealed neutralizing anti-SARS-CoV-2 S antibodies in the sera of ~90% of mildly and 100% of severely affected COVID-19 convalescent patients.Importantly,sVNT and pVNT results correlated strongly with each other and to the levels of anti-SARS-CoV-2 S1 IgG and IgA antibodies.Moreover,levels of neutralizing antibodies correlated with the duration and severity of clinical symptoms but not with patient age.Compared to pVNT,sVNT is less sophisticated and does not require any biosafety labs.Since this assay is also much faster and cheaper,sVNT will not only be important for evaluating the prevalence of neutralizing antibodies in a population but also for identifying promising plasma donors for successful passive antibody therapy.

Identification of fungal fossils and novel azaphilone pigments in ancient carbonised specimens of Hypoxylon fragiforme from forest soils of Chatillon-sur-Seine(Burgundy)

Fungal stromata were recently discovered in association with charcoal and burnt soil aggregates during an archaeological survey in the Chaˆtillon-sur-Seine forest massif.The wood and soil in the samples were dated to the medieval period(between 738 and 1411 AD).Light microscopy and scanning electron microscopy revealed that a few of the stromatal fragments still contained ascospores.Their macromorphological characters were described and secondary metabolite profiles were generated using high performance liquid chromatography with diode array and mass spectrometric detection(HPLC–DAD/MS).The combination of these two data lines then allowed species identification.Most of the fragments were assigned to Hypoxylon fragiforme,the type species of the Hypoxylaceae(Xylariales).Two further species whose stromata grew on the fossil charcoal could be tentatively identified as Jackrogersella cohaerens and(more tentatively)as Hypoxylon vogesiacum.These three species are still commonly encountered in the forests of Central Europe today.Furthermore,the HPLC-HRMS data of H.fragiforme suggested the presence of unknown azaphilone dimers and of further new pigments.These archaeological compounds were compared to fresh stromata of H.fragiforme collected in Germany and subjected to the same analytical protocol.While the major components in both samples were identified as the known mitorubrin type azaphilones and orsellinic acid,the chemical structures of seven novel complex azaphilone pigments,for which we propose the trivial names rutilins C-D and fragirubrins A-E,were elucidated using spectral methods(NMR and CD spectroscopy,high resolution mass spectrometry).It appears that these pigments had indeed persisted for millennia in the fossil stromata.

High quality genome sequences of thirteen Hypoxylaceae(Ascomycota)strengthen the phylogenetic family backbone and enable the discovery of new taxa

The Hypoxylaceae(Xylariales,Ascomycota)is a diverse family of mainly saprotrophic fungi,which commonly occur in angiosperm-dominated forests around the world.Despite their importance in forest and plant ecology as well as a prolific source of secondary metabolites and enzymes,genome sequences of related taxa are scarce and usually derived from envi-ronmental isolates.To address this lack of knowledge thirteen taxonomically well-defined representatives of the family and one member of the closely related Xylariaceae were genome sequenced using combinations of Illumina and Oxford nanopore technologies or PacBio sequencing.The workflow leads to high quality draft genome sequences with an average N50 of 3.0 Mbp.A backbone phylogenomic tree was calculated based on the amino acid sequences of 4912 core genes reflecting the current accepted taxonomic concept of the Hypoxylaceae.A Percentage of Conserved Proteins(POCP)analysis revealed that 70%of the proteins are conserved within the family,a value with potential application for the definition of family boundaries within the order Xylariales.Also,Hypomontagnella spongiphila is proposed as a new marine derived lineage of Hypom.monticulosa based on in-depth genomic comparison and morphological differences of the cultures.The results showed that both species share 95%of their genes corresponding to more than 700 strain-specific proteins.This difference is not reflected by standard taxonomic assessments(morphology of sexual and asexual morph,chemotaxonomy,phylogeny),preventing species delimitation based on traditional concepts.Genetic changes are likely to be the result of environmental adaptations and selective pressure,the driving force of speciation.These data provide an important starting point for the establishment of a stable phylogeny of the Xylariales;they enable studies on evolution,ecological behavior and biosynthesis of natural products;and they significantly advance the taxonomy of fungi.

Omicron spike protein:a clue for viral entry and immune evasion

In a recent study in Science,Bowen et al.1 report that mutations in Omicron sublineage’s spike protein enhance ACE2 binding,impair fusogenicity,and dampen the neutralizing activity of antibodies after vaccination or infection.The data describe how Omicron and especially the BA.5 variant evade neutralizing antibody responses and argue for using mRNA booster vaccination to increase immunity.

Taxonomic and phylogenetic contributions to fungi associated with the invasive weed Chromolaena odorata (Siam weed)

This article provides morphological descriptions and illustrations of microfungi associated with the invasive weed,Chro-molaena odorata,which were mainly collected in northern Thailand.Seventy-seven taxa distributed in ten orders,23 families(of which Neomassarinaceae is new),12 new genera(Chromolaenicola,Chromolaenomyces,Longiappendispora,Pseudocapulatispora,Murichromolaenicola,Neoophiobolus,Paraleptospora,Pseudoroussoella,Pseudostaurosphaeria,Pseudothyridariella,Setoarthopyrenia,Xenoroussoella),47 new species(Aplosporella chromolaenae,Arthrinium chromolae-nae,Chromolaenicola chiangraiensis,C.lampangensis,C.nanensis,C.thailandensis,Chromolaenomyces appendiculatus,Diaporthe chromolaenae,Didymella chromolaenae,Dyfrolomyces chromolaenae,Leptospora chromolaenae,L.phraeana,Longiappendispora chromolaenae,Memnoniella chromolaenae,Montagnula chiangraiensis,M.chromolaenae,M.chromo-laenicola,M.thailandica,Murichromolaenicola chiangraiensis,M.chromolaenae,Muyocopron chromolaenae,M.chromo-laenicola,Neomassarina chromolaenae,Neoophiobolus chromolaenae,Neopyrenochaeta chiangraiensis,N.chromolaenae,N.thailandica,N.triseptatispora,Nigrograna chromolaenae,Nothophoma chromolaenae,Paraleptospora chromolaenae,P.chromolaenicola,Patellaria chromolaenae,Pseudocapulatispora longiappendiculata,Pseudoroussoella chromolaenae,Pseudostaurosphaeria chromolaenae,P.chromolaenicola,Pseudothyridariella chromolaenae,Pyrenochaetopsis chromolae-nae,Rhytidhysteron chromolaenae,Setoarthopyrenia chromolaenae,Sphaeropsis chromolaenicola,Tremateia chiangraiensis,T.chromolaenae,T.thailandensis,Xenoroussoella triseptata,Yunnanensis chromolaenae),12 new host records,three new taxonomic combinations(Chromolaenicola siamensis,Pseudoroussoella elaeicola,Pseudothyridariella mahakashae),and two reference specimens(Torula chromolaenae,T.fici)are described and illustrated.Unlike some other hosts,e.g.bamboo(Poaceae)and Pandanaceae,the dominant group of fungi on Siam weed were Dothideomycetes.Only 15 species previously recorded from northern Thailand were found in this study.Most of the taxa are likely to have jumped hosts from surrounding plants and are unlikely to be a specialist to Siam weed.Most fungal families found on Siam weed had divergence estimates with stem ages in the Cretaceous,which coincided with the expected origin of the host family(Asteraceae).This further indicates that the species have jumped hosts,as it is unlikely that the taxa on the alien Siam weed came from the Americas with its host.They may,however,have jumped from other Asteraceae hosts.In a preliminary screening 40(65%)of the 62 species tested showed antimicrobial activity and thus,the fungi associated with C.odorata may be promising sources of novel bioactive compound discovery.We provide a checklist of fungi associated with C.odorata based on the USDA Systematic Mycology and Microbiology Laboratory(SMML)database,relevant literature and our study.In total,130 taxa(116 identified and 14 unidentified species)are distributed in 20 orders,48 families and 85 genera.Pseudocercospora is the most commonly encountered genus on Siam weed.

The world’s ten most feared fungi

An account is provided of the world’s ten most feared fungi.Within areas of interest,we have organized the entries in the order of concern.We put four human pathogens first as this is of concern to most people.This is followed by fungi producing mycotoxins that are highly harmful for humans;Aspergillus flavus,the main producer of aflatoxins,was used as an example.Problems due to indoor air fungi may also directly affect our health and we use Stachybotrys chartarum as an example.Not everyone collects and eats edible mushrooms.However,fatalities caused by mushroom intoxications often make news headlines and therefore we include one of the most poisonous of all mushrooms,Amanita phalloides,as an example.We then move on to the fungi that damage our dwellings causing serious anxiety by rotting our timber structures and flooring.Serpula lacrymans,which causes dry rot is an excellent example.The next example serves to represent all plant and forest pathogens.Here we chose Austropuccinia psidii as it is causing devastating effects in Australia and will probably do likewise in New Zealand.Finally,we chose an important amphibian pathogen which is causing serious declines in the numbers of frogs and other amphibians worldwide.Although we target the top ten most feared fungi,numerous others are causing serious concern to human health,plant production,forestry,other animals and our factories and dwellings.By highlighting ten feared fungi as an example,we aim to promote public awareness of the cost and importance of fungi.

Integrative approaches for species delimitation in Ascomycota

Biodiversity loss from disturbances caused by human activities means that species are disappearing at an ever increasing rate.The high number of species that have yet to be described have generated extreme crisis to the taxonomist.Therefore,more than in any other era,effective ways to discover and delimitate species are needed.This paper reviews the historically fore-most approaches used to delimit species in Ascomycota,the most speciose phylum of Fungi.These include morphological,biological,and phylogenetic species concepts.We argue that a single property to delineate species boundaries has various defects and each species concept comes with its own advantages and disadvantages.Recently the rate of species discovery has increased because of the advancement of phylogenetic approaches.However,traditional phylogenetic methods with few gene regions lack species-level resolution,and do not allow unambiguous conclusions.We detail the processes that affect gene tree heterogeneity,which acts as barriers to delimiting species boundaries in classical low-rank phylogenies.So far,limited insights were given to the DNA-based methodologies to establish well-supported boundaries among fungal species.In addition to reviewing concepts and methodologies used to delimit species,we present a case study.We applied differ-ent species delimitation methods to understand species boundaries in the plant pathogenic and cryptic genus Phyllosticta(Dothideomycetes,Botryosphaeriales).Several DNA-based methods over-split the taxa while in some methods several taxa fall into a single species.These problems can be resolved by using multiple loci and coalescence-based methods.Further,we discuss integrative approaches that are crucial for understanding species boundaries within Ascomycota and provide several examples for ideal and pragmatic approaches of species delimitation.

Families of Sordariomycetes

Sordariomycetes is one of the largest classes of Ascomycota that comprises a highly diverse range of fungi characterized mainly by perithecial ascomata and inoperculate unitunicate asci.The class includes many important plant pathogens,as well as endophytes,saprobes,epiphytes,coprophilous and fungicolous,lichenized or lichenicolous taxa.They occur in terrestrial,freshwater and marine habitats worldwide.This paper reviews the 107 families of the class Sordariomycetes and provides a modified backbone tree based on phylogenetic analysis of four combined loci,with a maximum five representative taxa from each family,where available.This paper brings together for the first time,since Barrs’1990 Prodromus,descriptions,notes on the history,and plates or illustrations of type or representative taxa of each family,a list of accepted genera,including asexual genera and a key to these taxa of Sordariomycetes.Delineation of taxa is supported where possible by molecular data.The outline is based on literature to the end of 2015 and the Sordariomycetes now comprises six subclasses,32 orders,105 families and 1331 genera.The family Obryzaceae and Pleurotremataceae are excluded from the class.

Neutralization of the SARS-CoV-2 Delta variant after heterologous and homologous BNT162b2 or ChAdOx1 nCoV-19 vaccination

Since the beginning of the COVID-19 pandemic,divergent variants of concern(VoCs)of SARS-CoV-2 have evolved and become the most prevalent SARS-CoV-2 variants in distinct locations at different times.Currently,the Delta variant(B.1.617.2)dominates infection events in large parts of the world.Immunization campaigns,however,still use SARS-CoV-2 vaccines based on the spike(S)protein of the original Wuhan virus.

Efficient antibody evasion but reduced ACE2 binding by the emerging SARS-CoV-2 variant B.1.640.2

The SARS-CoV-2 spike(S)protein engages ACE2 for cell entry,and the S protein/ACE2 interface is an important target for neutralizing antibodies.In the course of the COVID-19 pandemic,SARS-CoV-2 variants have emerged that harbor mutations in the S protein,which confer neutralization resistance and allow viral spread in immunologically nonnaive populations.The most prominent example is the highly mutated Omicron variant,which infects convalescent or vaccinated individuals with unprecedented efficiency[1,2].

Special issue:a tribute to Kevin D.Hyde on his 65th birthday

This special issue is dedicated to Emeritus Prof.Dr.Kevin D.Hyde in recognition of his contributions to mycology on the occasion of his 65th birthday.He was born on 3rd May 1955.As a young man,his curiosity about animals and plants around his locality and gathering and identifying mushrooms shaped his later career as a great mycologist in the present era.With an early desire to pursue biology,he obtained B.Sc.in Zoology in 1979 from University of Wales,Cardiff,UK,his B.Sc.research project being on duck nematodes.He became interested in the degradation of materials by microorganisms,and enrolled on a taught M.Sc.in the Biodeterioration of Materials at University of Portsmouth,UK,in 1979.As part of the course,he undertook a dissertation in entomology.At Portsmouth,Kevin first met Professor Gareth Jones,who shaped much of his career thereafter.With his passion for teaching and sharing knowledge with others,Kevin took a postgradu-ate certificate in education(PGCE)at Bristol University。

The amazing potential of fungi:50 ways we can exploit fungi industrially

Fungi are an understudied,biotechnologically valuable group of organisms.Due to the immense range of habitats that fungi inhabit,and the consequent need to compete against a diverse array of other fungi,bacteria,and animals,fungi have developed numerous survival mechanisms.The unique attributes of fungi thus herald great promise for their application in biotechnology and industry.Moreover,fungi can be grown with relative ease,making production at scale viable.The search for fungal biodiversity,and the construction of a living fungi collection,both have incredible economic potential in locating organisms with novel industrial uses that will lead to novel products.This manuscript reviews fifty ways in which fungi can potentially be utilized as biotechnology.We provide notes and examples for each potential exploitation and give examples from our own work and the work of other notable researchers.We also provide a flow chart that can be used to convince funding bodies of the importance of fungi for biotechnological research and as potential products.Fungi have provided the world with penicillin,lovastatin,and other globally significant medicines,and they remain an untapped resource with enormous industrial potential.

Fungal diversity notes 253-366:taxonomic and phylogenetic contributions to fungal taxa

Notes on 113 fungal taxa are compiled in this paper,including 11 new genera,89 new species,one new subspecies,three new combinations and seven reference specimens.Awide geographic and taxonomic range of fungal taxa are detailed.In the Ascomycota the new genera Angustospora(Testudinaceae),Camporesia(Xylariaceae),Clematidis,Crassiparies(Pleosporales genera incertae sedis),Farasanispora,Longiostiolum(Pleosporales genera incertae sedis),Multilocularia(Parabambusicolaceae),Neophaeocryptopus(Dothideaceae),Parameliola(Pleosporales genera incertae sedis),and Towyspora(Lentitheciaceae)are introduced.Newly introduced species are Angustospora nilensis,Aniptodera aquibella,Annulohypoxylon albidiscum,Astrocystis thailandica,Camporesia sambuci,Clematidis italica,Colletotrichum menispermi,C.quinquefoliae,Comoclathris pimpinellae,Crassiparies quadrisporus,Cytospora salicicola,Diatrype thailandica,Dothiorella rhamni,Durotheca macrostroma,Farasanispora avicenniae,Halorosellinia rhizophorae,Humicola koreana,Hypoxylon lilloi,Kirschsteiniothelia tectonae,Lindgomyces okinawaensis,Longiostiolum tectonae,Lophiostoma pseudoarmatisporum,Moelleriella phukhiaoensis,M.pongdueatensis,Mucoharknessia anthoxanthi,Multilocularia bambusae,Multiseptospora thysanolaenae,Neophaeocryptopus cytisi,Ocellularia arachchigei,O.ratnapurensis,Ochronectria thailandica,Ophiocordyceps karstii,Parameliola acaciae,P.dimocarpi,Parastagonospora cumpignensis,Pseudodidymosphaeria phlei,Polyplosphaeria thailandica,Pseudolachnella brevifusiformis,Psiloglonium macrosporum,Rhabdodiscus albodenticulatus,Rosellinia chiangmaiensis,Saccothecium rubi,Seimatosporium pseudocornii,S.pseudorosae,Sigarispora ononidis and Towyspora aestuari.New combinations are provided for Eutiarosporella dactylidis(sexual morph described and illus trated)and Pseudocamarosporium pini.Descriptions,illustrations and/or reference specimens are designated for Aposphaeria corallinolutea,Cryptovalsa ampelina,Dothiorella vidmadera,Ophiocordyceps formosana,Petrakia echinata,Phragmoporthe conformis and Pseudocamarosporium pini.The new species of Basidiomycota are Agaricus coccyginus,A.luteofibrillosus,Amanita atrobrunnea,A.digitosa,A.gleocystidiosa,A.pyriformis,A.strobilipes,Bondarzewia tibetica,Cortinarius albosericeus,C.badioflavidus,C.dentigratus,C.duboisensis,C.fragrantissimus,C.roseobasilis,C.vinaceobrunneus,C.vinaceogrisescens,C.wahkiacus,Cyanoboletus hymenoglutinosus,Fomitiporia atlantica,F.subtilissima,Ganoderma wuzhishanensis,Inonotus shoreicola,Lactifluus armeniacus,L.ramipilosus,Leccinum indoaurantiacum,Musumecia alpina,M.sardoa,Russula amethystina subp.tengii and R.wangii are introduced.Descriptions,illustrations,notes and/or reference specimens are designated for Clarkeinda trachodes,Dentocorticium ussuricum,Galzinia longibasidia,Lentinus stuppeus and Leptocorticium tenellum.The other new genera,species new combinations are Anaeromyces robustus,Neocallimastix californiae and Piromyces finnis from Neocallimastigomycota,Phytophthora estuarina,P.rhizophorae,Salispina,S.intermedia,S.lobata and S.spinosa from Oomycota,and Absidia stercoraria,Gongronella orasabula,Mortierella calciphila,Mucor caatinguensis,M.koreanus,M.merdicola and Rhizopus koreanus in Zygomycota.