Vidangadi churna is a popular Ayurvedic formulation described in the chapter Krimicikitsa of the Ayurvedic literature Cakradatta for the treatment of Krimiroga. The preparation is a composite mixture of the fine powde...Vidangadi churna is a popular Ayurvedic formulation described in the chapter Krimicikitsa of the Ayurvedic literature Cakradatta for the treatment of Krimiroga. The preparation is a composite mixture of the fine powder of fruits of Vidang （Embelia ribs）, glandular trichomes of the fruits of Kamala （Mallotus philippensis）, mature fruits of Harde （Terminalia chehula）, Saindhava and Yavakshara. The use of reversed phase C18 column eluted with gradient mobile phase of acetonitrile and water enabled the efficient separation of the chemical markers in 22 rain. Validation of the method was performed in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery. All calibration curves showed good linear correlation coefficients （r2〉0.995） within the tested ranges. Three markers in Vidangadi churna were quantitied with respect to Embelin （0.647%, w/w）, Rottlerin （4.419%, w/w）, and Ellagic acid （0.459%, w/w）. lntraand inter-day RSDs of retention times and peak areas were less than 3.12%. The recoveries were between 99.66% and 102.33%. In conclusion, a method has been developed for the simultaneous quantification of three markers in Vidangadi churna. The RP-HPLC method was simple, precise and accurate and can be used for the quality control of the raw materials as well as formulations.展开更多
Nanozymes are the paradigm for bridging inorganic nanomaterials with biology and environment for taking the spontaneous responsibilities to outplay natural enzymes.Metal-organic frameworks(MOFs) are mesoporous materia...Nanozymes are the paradigm for bridging inorganic nanomaterials with biology and environment for taking the spontaneous responsibilities to outplay natural enzymes.Metal-organic frameworks(MOFs) are mesoporous materials of inorganic-organic coordination,bearing ampoules of active/target sites and having the tendency to mimic natural enzymes.Thus MOF-based nanozymes(NZs) could be recognized for their tremendous potential for bio-catalysis.However,MOFs are of four types namely: modified MOFs,pristine MOFs,MOF-derived materials and MOFs comprised of natural enzymes.The MOFs-based NZ modulated via ultrasound,light,and heat revealed diversified applications.This article is concentrated on different methods for the preparation of MOF-based NZ for mimicking the responses of catalases,multifunctional enzymes,oxidases,superoxide dismutase,hydrolases,and peroxidases,progress and challenges of MOFs/MOF-based materials for exploiting their recent and futuristic approaches in biomedical sector.展开更多
文摘Vidangadi churna is a popular Ayurvedic formulation described in the chapter Krimicikitsa of the Ayurvedic literature Cakradatta for the treatment of Krimiroga. The preparation is a composite mixture of the fine powder of fruits of Vidang （Embelia ribs）, glandular trichomes of the fruits of Kamala （Mallotus philippensis）, mature fruits of Harde （Terminalia chehula）, Saindhava and Yavakshara. The use of reversed phase C18 column eluted with gradient mobile phase of acetonitrile and water enabled the efficient separation of the chemical markers in 22 rain. Validation of the method was performed in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery. All calibration curves showed good linear correlation coefficients （r2〉0.995） within the tested ranges. Three markers in Vidangadi churna were quantitied with respect to Embelin （0.647%, w/w）, Rottlerin （4.419%, w/w）, and Ellagic acid （0.459%, w/w）. lntraand inter-day RSDs of retention times and peak areas were less than 3.12%. The recoveries were between 99.66% and 102.33%. In conclusion, a method has been developed for the simultaneous quantification of three markers in Vidangadi churna. The RP-HPLC method was simple, precise and accurate and can be used for the quality control of the raw materials as well as formulations.
文摘Nanozymes are the paradigm for bridging inorganic nanomaterials with biology and environment for taking the spontaneous responsibilities to outplay natural enzymes.Metal-organic frameworks(MOFs) are mesoporous materials of inorganic-organic coordination,bearing ampoules of active/target sites and having the tendency to mimic natural enzymes.Thus MOF-based nanozymes(NZs) could be recognized for their tremendous potential for bio-catalysis.However,MOFs are of four types namely: modified MOFs,pristine MOFs,MOF-derived materials and MOFs comprised of natural enzymes.The MOFs-based NZ modulated via ultrasound,light,and heat revealed diversified applications.This article is concentrated on different methods for the preparation of MOF-based NZ for mimicking the responses of catalases,multifunctional enzymes,oxidases,superoxide dismutase,hydrolases,and peroxidases,progress and challenges of MOFs/MOF-based materials for exploiting their recent and futuristic approaches in biomedical sector.
