Objective： To investigate the mechanism of norcantharidin （NCTD）-induced SMMC-7721 hepatoma cell apoptosis. Methods： SMMC-7721 cell growth inhibition was measured by the MTT method. Apoptosis was detected by Annex...Objective： To investigate the mechanism of norcantharidin （NCTD）-induced SMMC-7721 hepatoma cell apoptosis. Methods： SMMC-7721 cell growth inhibition was measured by the MTT method. Apoptosis was detected by Annexin Ⅴ/propidium iodide staining. The mitochondrial membrane potential was measured by flow cytometry. Western blot analysis was used to evaluate the level of cytochrome c, caspase-3, AIF, Bcl-2 and Bax expression. Results： NCTD inhibited SMMC-7721 cell growth in a time-and dose-dependent manner. The cells treated with NCTD showed the loss of mitochondrial membrane potential. The activities of caspase-3, cytochrome c, AIF, and Bax were up-regulated after NCTD treatment at different doses. The expression of Bcl-2 was decreased after treatment with NCTD. Conclusions： NCTD could induce SMMC-7721 cell apoptosis. The activation of the mitochondrial pathway was involved in the process of NCTD-induced SMMC-7721 cell apoptosis.展开更多
基金Supported by National Natural Science Foundation of China(No. 2007Z002A)Grants of Putuo Hospital Affiliated to Shanghai University of Traditional Chinese MedicineGrants from the Department of Biochemistry and Molecular Biology,Department of Hematology and Oncology, University of MiamiMiller School of Medicine
文摘Objective： To investigate the mechanism of norcantharidin （NCTD）-induced SMMC-7721 hepatoma cell apoptosis. Methods： SMMC-7721 cell growth inhibition was measured by the MTT method. Apoptosis was detected by Annexin Ⅴ/propidium iodide staining. The mitochondrial membrane potential was measured by flow cytometry. Western blot analysis was used to evaluate the level of cytochrome c, caspase-3, AIF, Bcl-2 and Bax expression. Results： NCTD inhibited SMMC-7721 cell growth in a time-and dose-dependent manner. The cells treated with NCTD showed the loss of mitochondrial membrane potential. The activities of caspase-3, cytochrome c, AIF, and Bax were up-regulated after NCTD treatment at different doses. The expression of Bcl-2 was decreased after treatment with NCTD. Conclusions： NCTD could induce SMMC-7721 cell apoptosis. The activation of the mitochondrial pathway was involved in the process of NCTD-induced SMMC-7721 cell apoptosis.
