Acute kidney injury (AKI) is defined by 0.3 mg/dL increase in serum creatinine (SCr) and is associated with higher incidence of postoperative mortality after coronary artery bypass graft (CABG). There are few clinical...Acute kidney injury (AKI) is defined by 0.3 mg/dL increase in serum creatinine (SCr) and is associated with higher incidence of postoperative mortality after coronary artery bypass graft (CABG). There are few clinical studies on the effect of dexmedetomidine (DEX) on renal function. We evaluated AKI after coronary artery bypass graft with and without cardiopulmonary bypass (CPB) under anesthesia with DEX. Method: In this retrospective study, we performed serial analysis of serum creatinine (SCr) up to 48 hours after surgery in 286 patients who underwent CABG. We tested the similarities between groups, evaluating patients separately for use of CPB and DEX. Each patient was evaluated for his or her SCr at the following points in time: preoperative, immediately postoperative, 24 hours postoperative, and 48 hours postoperative. Preoperative SCr was used as the baseline value for each patient. If the SCr increased ≥0.3 mg/dL in at least one of the periods, the patient was classified as having AKI. We also assessed the risk for AKI in patients with altered preoperative SCr (values between 1.1 to 2.0 mg/dL for women or 1.3 to 2.0 mg/dL for men) compared to patients with normal SCr. Results: The groups were similar for preoperative weight, age, and altered SCr. Patients were anesthetized with DEX and who underwent CPB exhibited higher incidence of AKI (p = 0.043). Without CPB, there was higher incidence of AKI after using DEX (p = 0.066). Conclusion: Anaesthesia with DEX increased the incidence of AKI after myocardial revascularization surgery in patients who underwent CPB.展开更多
<strong>Objectives:</strong><span style="font-family:""><span style="font-family:Verdana;"> We aimed to determine the frequency of PTEN (phosphatase with tensin homolog...<strong>Objectives:</strong><span style="font-family:""><span style="font-family:Verdana;"> We aimed to determine the frequency of PTEN (phosphatase with tensin homology deleted in chromosome 10) hypermethylation in renal cell carcinoma (RCC) and its impact on overall (OS) and disease-free survival (DFS). The association between PTEN hypermethylation and clinic and pathologic factors was also analyzed. </span><b><span style="font-family:Verdana;">Materials and Methods: </span></b><span style="font-family:Verdana;">The authors analyzed 137 patients who had undergone a radical or partial nephrectomy between 1997 and 2011. The methylation pattern was quantified individually at multiple CpG islands that are located on the exon 1 of PTEN gene by pyrosequencing.</span></span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> Mean follow-up was 32</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;">3 months. PTEN hypermethylation was detected with a low frequency (3.6%). There were no deaths or disease recurrence among patients with PTEN hypermethylation. Determining the association between PTEN hypermethylation and clinical and pathological factors was not possible due to the low frequency observed.</span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> The results show that the methylation of promoter CpG sites in PTEN is relatively infrequent in renal carcinogenesis and has no prognostic impact on survival.</span>展开更多
Soft tissue sarcomas (STS) are rare neoplasm with frequencies around 1% of all neoplasms. Although it consists of a high heterogeneous group of tumors, surgery is the mean treatment. The STS surgery is still challengi...Soft tissue sarcomas (STS) are rare neoplasm with frequencies around 1% of all neoplasms. Although it consists of a high heterogeneous group of tumors, surgery is the mean treatment. The STS surgery is still challenging and complex procedure is usually required: this is because STS requires different types of resection and reconstruction due to various tissue-commitment</span><span style="font-family:"">s</span><span style="font-family:""> (nerve, arteries, skin and muscle). So, a multidisciplinary team must be prepared for STS approach to obtain the maximum local control and a limited extremity functional impairment. We, here, showing our experience, wish to introduce some technical contrivances in STS surgery, with special reference to tissue reconstruction. This may illustrate the necessity of a multidisciplinary team approach in this surgery.展开更多
Clear Cell Sarcoma (CCS) is a rare soft tissue sarcoma that corresponds <span style="font-family:Verdana;">to </span><span style="font-family:Verdana;">a less than 1% of all sarco...Clear Cell Sarcoma (CCS) is a rare soft tissue sarcoma that corresponds <span style="font-family:Verdana;">to </span><span style="font-family:Verdana;">a less than 1% of all sarcomas</span><span style="font-family:Verdana;"> and</span><span style="font-family:Verdana;"> is a high degree neoplasm with propensity to disseminate to regional lymph nodes. Regional lymphadenectomies have </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">controversial impact on sarcomas treatment and survival, even though those patients have higher local recurrence and lower survival. So, sentinel lymph node biopsy must be one option for better regional staging to STS with higher propensity to lymph node commitment. The authors demonstrate two cases of CCS submitted to surgery through compartmental resection associated </span><span style="font-family:Verdana;">with</span><span style="font-family:Verdana;"> the sentinel lymph node biopsy. A literature review about soft tissue sarcoma and lymph node sentinel biopsy </span><span style="font-family:Verdana;">is</span><span style="font-family:Verdana;"> show</span><span style="font-family:Verdana;">n </span><span style="font-family:Verdana;">as an initial experience of two cases.</span>展开更多
Knowing the physiology of seeds and the elements that influence their germination is fundamental aspects in seminiferous propagation;important techniques are used to obtain genetic variability and development of new c...Knowing the physiology of seeds and the elements that influence their germination is fundamental aspects in seminiferous propagation;important techniques are used to obtain genetic variability and development of new cultivars of blueberry. The aim of this study is to evaluate the germination behavior, as well as viability levels, through germination tests and tetrazolium, of Vaccinium ashei Reade seed cultivars Briteblue and Climax. Seeds treated or not with 5 M potassium hydroxide (KOH) were submitted to the germination test, on substrates, filter paper (SP) or solid culture medium with half of the salt concentration (MS/2), at temperatures of 10°C ± 2°C or 25°C ± 2°C. The maximum germination percentage of blueberry seeds was 40%. Both temperatures and substrates caused seed germination in the tested cultivars, and pretreatment with 5 M KOH for 5 minutes inhibited germination. Yet, the tetrazolium test, based on coloration of tissue, allowed the establishment of different levels of viability.展开更多
文摘Acute kidney injury (AKI) is defined by 0.3 mg/dL increase in serum creatinine (SCr) and is associated with higher incidence of postoperative mortality after coronary artery bypass graft (CABG). There are few clinical studies on the effect of dexmedetomidine (DEX) on renal function. We evaluated AKI after coronary artery bypass graft with and without cardiopulmonary bypass (CPB) under anesthesia with DEX. Method: In this retrospective study, we performed serial analysis of serum creatinine (SCr) up to 48 hours after surgery in 286 patients who underwent CABG. We tested the similarities between groups, evaluating patients separately for use of CPB and DEX. Each patient was evaluated for his or her SCr at the following points in time: preoperative, immediately postoperative, 24 hours postoperative, and 48 hours postoperative. Preoperative SCr was used as the baseline value for each patient. If the SCr increased ≥0.3 mg/dL in at least one of the periods, the patient was classified as having AKI. We also assessed the risk for AKI in patients with altered preoperative SCr (values between 1.1 to 2.0 mg/dL for women or 1.3 to 2.0 mg/dL for men) compared to patients with normal SCr. Results: The groups were similar for preoperative weight, age, and altered SCr. Patients were anesthetized with DEX and who underwent CPB exhibited higher incidence of AKI (p = 0.043). Without CPB, there was higher incidence of AKI after using DEX (p = 0.066). Conclusion: Anaesthesia with DEX increased the incidence of AKI after myocardial revascularization surgery in patients who underwent CPB.
文摘<strong>Objectives:</strong><span style="font-family:""><span style="font-family:Verdana;"> We aimed to determine the frequency of PTEN (phosphatase with tensin homology deleted in chromosome 10) hypermethylation in renal cell carcinoma (RCC) and its impact on overall (OS) and disease-free survival (DFS). The association between PTEN hypermethylation and clinic and pathologic factors was also analyzed. </span><b><span style="font-family:Verdana;">Materials and Methods: </span></b><span style="font-family:Verdana;">The authors analyzed 137 patients who had undergone a radical or partial nephrectomy between 1997 and 2011. The methylation pattern was quantified individually at multiple CpG islands that are located on the exon 1 of PTEN gene by pyrosequencing.</span></span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> Mean follow-up was 32</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;">3 months. PTEN hypermethylation was detected with a low frequency (3.6%). There were no deaths or disease recurrence among patients with PTEN hypermethylation. Determining the association between PTEN hypermethylation and clinical and pathological factors was not possible due to the low frequency observed.</span><span style="font-family:""> </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> The results show that the methylation of promoter CpG sites in PTEN is relatively infrequent in renal carcinogenesis and has no prognostic impact on survival.</span>
文摘Soft tissue sarcomas (STS) are rare neoplasm with frequencies around 1% of all neoplasms. Although it consists of a high heterogeneous group of tumors, surgery is the mean treatment. The STS surgery is still challenging and complex procedure is usually required: this is because STS requires different types of resection and reconstruction due to various tissue-commitment</span><span style="font-family:"">s</span><span style="font-family:""> (nerve, arteries, skin and muscle). So, a multidisciplinary team must be prepared for STS approach to obtain the maximum local control and a limited extremity functional impairment. We, here, showing our experience, wish to introduce some technical contrivances in STS surgery, with special reference to tissue reconstruction. This may illustrate the necessity of a multidisciplinary team approach in this surgery.
文摘Clear Cell Sarcoma (CCS) is a rare soft tissue sarcoma that corresponds <span style="font-family:Verdana;">to </span><span style="font-family:Verdana;">a less than 1% of all sarcomas</span><span style="font-family:Verdana;"> and</span><span style="font-family:Verdana;"> is a high degree neoplasm with propensity to disseminate to regional lymph nodes. Regional lymphadenectomies have </span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">controversial impact on sarcomas treatment and survival, even though those patients have higher local recurrence and lower survival. So, sentinel lymph node biopsy must be one option for better regional staging to STS with higher propensity to lymph node commitment. The authors demonstrate two cases of CCS submitted to surgery through compartmental resection associated </span><span style="font-family:Verdana;">with</span><span style="font-family:Verdana;"> the sentinel lymph node biopsy. A literature review about soft tissue sarcoma and lymph node sentinel biopsy </span><span style="font-family:Verdana;">is</span><span style="font-family:Verdana;"> show</span><span style="font-family:Verdana;">n </span><span style="font-family:Verdana;">as an initial experience of two cases.</span>
文摘Knowing the physiology of seeds and the elements that influence their germination is fundamental aspects in seminiferous propagation;important techniques are used to obtain genetic variability and development of new cultivars of blueberry. The aim of this study is to evaluate the germination behavior, as well as viability levels, through germination tests and tetrazolium, of Vaccinium ashei Reade seed cultivars Briteblue and Climax. Seeds treated or not with 5 M potassium hydroxide (KOH) were submitted to the germination test, on substrates, filter paper (SP) or solid culture medium with half of the salt concentration (MS/2), at temperatures of 10°C ± 2°C or 25°C ± 2°C. The maximum germination percentage of blueberry seeds was 40%. Both temperatures and substrates caused seed germination in the tested cultivars, and pretreatment with 5 M KOH for 5 minutes inhibited germination. Yet, the tetrazolium test, based on coloration of tissue, allowed the establishment of different levels of viability.