CIPK9: a calcium sensor-interacting protein kinase required for low-potassium tolerance in Arabidopsis

钾是为在植物的很多个细胞的过程的主要宏营养素必需品之一。在土壤的低钾水平为庄稼生产代表一个限制因子。最近的研究识别了涉及钾获得的钾运输 ers，并且他们中的一些为在低钾条件下面的钾营养是批评的。然而，很少在涉及低钾发信号和回答的分子的部件上被理解。我们这里在 Arabidopsis 作为低钾反应的一个批评管理者报导 calcineurin 象 B 一样交往蛋白质的 proteinkinase (CIPK9 ) 的鉴定。CIPK9 基因对不能生活的压力条件应答，并且它的抄本由钾剥夺在根和射击是可诱导的。CIPK9 功能的混乱使变异的植物变为过分敏感到低钾媒介。进一步的分析显示那 K (+) 举起和内容没在变异的植物被影响，暗示在钾利用的规定的 CIPK9 或察觉到过程。

Calcineurin-B-Like Protein CBL9 Interacts with Target Kinase CIPK3 in the Regulation of ABA Response in Seed Germination

Calcium plays a vital role as a second messenger in many signaling pathways in plants. The calcineurin B-like proteins （CBLs） represent a family of plant calcium-binding proteins that function in calcium signaling by interacting with their interacting protein kinases （CIPKs）. In our previous study, we have reported a role for one of the CBLs （CBL9） and one of the CIPKs （CIPK3） in ABA signaling. Here, we have shown that CBL9 and CIPK3 physically and functionally interact with each other in regulating the ABA responses. The CBL9 and CIPK3 proteins interacted with each other in the yeast two- hybrid system and when expressed in plant cells. The double mutant cbl9cipk3 showed the similar hypersensitive response to ABA as observed in single mutants （cbl9 or cipk3）. The constitutively active form of CIPK3 genetically complemented the cbl9 mutant, indicating that CIPK3 function downstream of CBL9. Based on these findings, we conclude that CBL9 and CIPK3 act together in the same pathway for regulating ABA responses.