Identification and characterization of FpRco1 in regulating vegetative growth and pathogenicity based on T-DNA insertion in Fusarium pseudograminearum

Fusarium pseudograminearum is a devastating pathogen that causes Fusarium crown rot(FCR)in wheat and poses a significant threat to wheat production in terms of grain yield and quality.However,the mechanism by which F.pseudograminearum infects wheat remains unclear.In this study,we aimed to elucidate these mechanisms by constructing a T-DNA insertion mutant library for the highly virulent strain WZ-8A of F.pseudograminearum.By screening this mutant library,we identified nine independent mutants that displayed impaired pathogenesis in barley leaves.Among these mutants,one possessed a disruption in the gene FpRCO1 that is an ortholog of Saccharomyces cerevisiae RCO1,encoding essential component of the Rpd3S histone deacetylase complex in F.pseudograminearum.To further investigate the role of FpRCO1 in F.pseudograminearum,we employed a split-marker approach to knock out FpRCO1 in F.pseudograminearum WZ-8A.FpRCO1 deletion mutants exhibit reduced vegetative growth,conidium production,and virulence in wheat coleoptiles and barley leaves,whereas the complementary strain restores these phenotypes.Moreover,under stress conditions,the FpRCO1 deletion mutants exhibited increased sensitivity to NaCl,sorbitol,and SDS,but possessed reduced sensitivity to H_(2)O_(2)compared to these characteristics in the wild-type strain.RNA-seq analysis revealed that deletion of FpRCO1 affected gene expression(particularly the downregulation of TRI gene expression),thus resulting in significantly reduced deoxynivalenol(DON)production.In summary,our findings highlight the pivotal role of FpRCO1 in regulating vegetative growth and development,asexual reproduction,DON production,and pathogenicity of F.pseudograminearum.This study provides valuable insights into the molecular mechanisms underlying F.pseudograminearum infection in wheat and may pave the way for the development of novel strategies to combat this devastating disease.

Parametric Analysis of A Submerged Cylindrical Shell Subjected to Shock Waves

In this study, an FEM-SBFEM （scaled boundary finite element method） coupling procedure proposed by Fan et al. （2005） is adopted to obtain the dynamic responses of a submerged cylindrical shell subjected to plane step or exponential acoustic shock waves. The coupling procedure can readily be applied to three-dimensional problem, however for clarity, the problems to be presented are hmited to two-dimeusional domain. In the analyses, the cylindrical shell is modeled by simple beam elements （using FEM）, while the effects of the surrounding infinite fluid is modeled by the SBFEM. In it, no free surface and seabed are involved. Compared with Fan and his co-authors＇ works, the FEM-SBFEM coupling procedure is further verified to be feasible for shock waves by benchmark examples. Furthermore, parametric studies are performed and presented to gain insight into effects of the geometric and material properties of the cylindrical shell on its dynamic responses.

Oblique perforation of thick metallic plates by rigid projectiles

Oblique perforation of thick metallic plates by rigid projectiles with various nose shapes is studied in this paper. Two perforation mechanisms, i.e., the hole enlargement for a sharp projectile nose and the plugging formation for a blunt projectile nose, are considered in the proposed analytical model. It is shown that the perforation of a thick plate is dominated by several non-dimensional numbers, i.e., the impact function, the geometry function of projectile, the non-dimensional thickness of target and the impact obliquity. Explicit formulae are obtained to predict the ballistic limit, residual velocity and directional change for the oblique perforation of thick metallic plates. The proposed model is able to predict the critical condition for the occurrence of ricochet. The proposed model is validated by comparing the predictions with other existing models and independent experimental data.

Research and design of CRT-based homomorphic ciphertext database system

The cloud’s storage and query of private information have the cryptographic scholar due to the proliferation of cloud computing.In the traditional query mode,the private information stored in the cloud is at risk of being leaked.In order to solve this problem,a cloud ciphertext database system based on homomorphic encryption is a valid workaround.This paper presents a new cloud ciphertext database system model,which is based on the existing ciphertext database mode research and homomorphic properties.This paper also implements a ciphertext database system based on a CRT-based additive homomorphic scheme according to the model.Through theoretical analysis,the model is CPA-level safe and correct.The experimental results show that users can correctly query and download the data in the ciphertext database on the untrusted cloud server through the model,and it has efficiency advantages.

The bubble formation characteristics of oil‐paper insulation and its influence on insulation performance

Engineering experience shows that for transformers with long service life and serious moisture exposure,the inter‐turn paper insulation inside the winding will precipitate bubbles under the condition of electric field and conductor heating,which will endanger the equipment insulation.In order to explore the influence factors of bubbles formation in the moist insulating paper of transformers,based on the existing research on the influence of moisture content and gas dissolution in transformer oil,this study further considers the influence of air pressure(AP)and electric field and establishes an experimental platform to change the air pressure and electric field,thereby studying the formation characteristics of bubbles in oil‐paper insulation.The results show that air pressure and electric field can affect the initial temperature,volume,and shape of bubble formation.From 0 to−0.05 MPa for air pressure,the initial temperature of bubble generation decreased by 41℃,its maximum two‐dimensional projected area increased by 418.4%with more severe distortion,and the breakdown voltage decreased.When the AC voltage applied on the needle plate electrode increased from 0 to 10 kV,the initial temperature of bubble formation increased by 29℃,and the maximum two‐dimensional projection area increased by 336.6%.

Leap of Li Metal Anodes from Coin Cells to Pouch Cells:Challenges and Progress

Li metal anodes have attracted tremendous attention in the last decade because of their high theoretical capacities and low electrochemical potentials.However,until now,there has only been limited success in improving the interfacial and structural stabilities and in realizing the highly controllable and large-scale fabrication of this emerging material;these limitations have posed great obstacles to further performing fundamental and applied studies in Li metal anodes.In this review,we focus on summarizing the existing challenges of Li metal anodes based on the leap from coin cells to pouch cells and on outlining typical methods for designing Li metal anodes on demand;we controllably engineer their surface protection layers and structure sizes by encapsulating structured Li metal inside a variety of synthetic protection layers.We aim to provide a comprehensive understanding and serve as a strategic guide for designing and fabricating practicable Li metal anodes for use in pouch batteries.Challenges and opportunities regarding this burgeoning field are critically evaluated at the end of this review.

One-step preparation of modified photothermal-driven melamine foam with gradient wettability for oil-waterseparation

The absorption of high-viscosity oil by traditional oil absorbing materials has always been a challenge.So there is an urgent need to solve the problem of slow absorption of high-viscosity oil.In this work,an emulsion composed of polydimethylsiloxane(PDMS),carbon black(CB)and waterborne polyurethane(solid content 40%)was sprayed on the melamine foam(MF).After volatilization of organic solvents,the photothermal material CB was fixed on the MF framework,making it photothermal.By raising the temperature of the modified foam to accelerate the internal thermal movement of high-viscosity oil molecules around the foam,intermolecular forces are reduced,thereby accelerating the separation process.The absorption capacity of this modified MF towards organic solvents and oil is up to 79 times its own weight.In addition,the mechanical properties of the modified foam are improved to a certain extent,more conducive to the continuous oil-water separation.This photothermal absorption material provides ideas for the rapid removal of high-viscosity oil,heavy oil,etc.