Cytokines are a broad class of protein cellregulators. Cytokines have been variouslytermed monokines, lymphokines, interleukins,growth factors, and biological responsemodifiers. These proteins have many charac-teristi...Cytokines are a broad class of protein cellregulators. Cytokines have been variouslytermed monokines, lymphokines, interleukins,growth factors, and biological responsemodifiers. These proteins have many charac-teristics in common. They are low molecularweight (less than 80kD) polypeptides, oftenglycosylated, act at short range in an autocrineor a paracrine rather than an endocrine fa-shion, and all interact with the extracellulardomains of transmembrane cellular receptorsspecific for each cytokine or cytokine group.Interaction between a cytokine and展开更多
Dengue virus type 2 ThNH7/93 retained infectious activity after purification by ceramic hydroxyapatite chromatogra-phy. Dengue virus type 2 culture fluid was loaded onto the ceramic hydroxyapatite column and eluted wi...Dengue virus type 2 ThNH7/93 retained infectious activity after purification by ceramic hydroxyapatite chromatogra-phy. Dengue virus type 2 culture fluid was loaded onto the ceramic hydroxyapatite column and eluted with a linear gradient of sodium phosphate buffer. Culture fluid and protein contaminants derived from host cells were eluted initially, followed by elutions of dsDNA, and then dengue virus type 2. The recoveries of dengue virus type 2 were 64 ± 14% (n = 11) in the hemagglutination (HA) test and 60% (n = 2) determined by focus assay for viral infectivity. This protocol was highly reproducible, simple, rapid, and appears applicable to other virus species such as influenza virus, Japanese encephalitis virus and adenovirus.展开更多
文摘Cytokines are a broad class of protein cellregulators. Cytokines have been variouslytermed monokines, lymphokines, interleukins,growth factors, and biological responsemodifiers. These proteins have many charac-teristics in common. They are low molecularweight (less than 80kD) polypeptides, oftenglycosylated, act at short range in an autocrineor a paracrine rather than an endocrine fa-shion, and all interact with the extracellulardomains of transmembrane cellular receptorsspecific for each cytokine or cytokine group.Interaction between a cytokine and
文摘Dengue virus type 2 ThNH7/93 retained infectious activity after purification by ceramic hydroxyapatite chromatogra-phy. Dengue virus type 2 culture fluid was loaded onto the ceramic hydroxyapatite column and eluted with a linear gradient of sodium phosphate buffer. Culture fluid and protein contaminants derived from host cells were eluted initially, followed by elutions of dsDNA, and then dengue virus type 2. The recoveries of dengue virus type 2 were 64 ± 14% (n = 11) in the hemagglutination (HA) test and 60% (n = 2) determined by focus assay for viral infectivity. This protocol was highly reproducible, simple, rapid, and appears applicable to other virus species such as influenza virus, Japanese encephalitis virus and adenovirus.
