Changes of selenium content in hair samples of people in Kaschin- Beck disease areas two months after stopping selenium salt in Shaanxi Province in 2012

Objective To compare selenium content in hair samples of people in Kaschin-Beck disease(KBD)areas and non KBD areas two months after stopping selenium salt in Shaanxi Province,to provide a scientific basis for KBD control and prevention.Methods In September2012,four historical KBD areas were selected,including Yongshou,Yuyang,Linyou and Nanzheng,four

A comparative study on the difference of gut microbiota and its biomarkers between patients with pulmonary tuberculosis and healthy controls

Objective To analyze the differences in the composition and abundance of gut microbiota between patients with active pulmonary tuberculosis and healthy controls,and to identify the specific bacteria as biomarkers to distinguish between the two groups.Methods Patients with active pulmonary tuberculosis treated in three municipal designated tuberculosis medical institutions in Sichuan,Jiangsu and Shanghai from September 2017 to September 2019 were selected as case group(n=88),and the healthy people without a history of tuberculosis from the same regions were recruited as control group(n=62).

Intra-host Ebola viral adaption during human infection

The onsite next generation sequencing(NGS)of Ebola virus(EBOV)genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin,transmission,and evolution of this virus.Herein,we have diagnosed a cohort of EBOV patients in Sierra Leone in 2015,during the late phase of the outbreak.The surviving EBOV patients had a recovery process characterized by decreasing viremia,fever,and biochemical parameters.EBOV genomes sequenced through the longitudinal blood samples of these patients showed dynamic intra-host substitutions of the virus during acute infection,including the previously described short stretches of 13 serial TNC mutations.Remarkably,within individual patients,samples collected during the early phase of infection possessed Ts at these nucleotide sites,whereas they were replaced by Cs in samples collected in the later phase,suggesting that these short stretches of TNC mutations could emerge independently.In addition,up to a total of 35 nucleotide sites spanning the EBOV genome were mutated coincidently.Our study showed the dynamic intra-host adaptation of EBOV during patient recovery and gave more insight into the complex EBOV-host interactions.

The effects of fluoride on testicular cell cycle and cell apoptosis of male rats

Objective To observe the effects of fluoride on testicular cell cycle and cell apoptosis of male rats.Methods Thirty-two healthy male Wistar rats,weighting 150-180 g,were randomly divided into 4 groups by body weight using random number table,normal sodium(control),the low-dose,medium-dose and high-dose groups(100,200,300 mg·kg-1·d-1Na F,respectively)by intragastric administration for 90 days,and

Combined effects of fluoride and arsenite on the expression of Runx-related transcription 2 mRNA in bone of rats

Objective To explore the combined effects of fluoride and arsenite on the expression of Runx-related transcription 2(Runx2)mRNA in bone of Sprague Dawley(SD)rats.Methods Fifty four SD rats were selected[body mass(109.71±10.52)g,half male and half female].3×3 Factorial experimental design was used to evaluate the combined effects of fluoride and arsenite on the expression of Runx2 mRNA by random number table.

Effect of fluoride on expression of insulinlike growth factor-1 and its receptor of rat osteoblasts

Objective To explore the influence of fluorine on mRNA and protein expression of the insulin-like growth factor-1 (IGF-1) and its receptor of rat osteoblasts.Methods Osteoblasts were isolated from rat bone by enzyme digestion.Different fluorine concentration[0 (con-

Influence factors of salt-sensitive hypertension and responses of blood pressure and urinary sodium and potassium excretion to acute oral saline loading among essential hypertensive patients

Objective To explore the influence factors of saltsensitive hypertension and to observe changes of blood pressures and urinary sodium and potassium excretion in response to acute oral saline loading among essential hypertensive patients in China.Methods Essential hypertensive patients from Beijing Jinzhan second community were included in this study.Salt-sensitivity was determined via the improved Sullivan’s acute oral saline

Disease burden of lung cancer in Jinchang cohort

Objective To understand the current status of lung cancer disease burden in Jinchang cohort.Methods In this historical cohort study,the mortality data of the lung cancer from 2001 to 2013 and medical records of the lung caner cases from 2001 to 2010 in Jinchang cohort were used,analyze mortality,direct economic burden,poten-

Disease burden of gastric cancer in Jinchang cohort

Objective To understand the current status of disease burden caused by gastric cancer in Jinchang cohort.Methods In this historical cohort study,the data of gastric cancer deaths from 2001 to 2013 and the medical records of gastric cancer cases from 2001 to 2010 in Jinchang cohort were collected to analyze the mortality,po-

Molecular characteristics and antibiotic resistance of Vibrio cholerae O139 in Shandong province

Objective To investigate the molecular epidemiological characteristics and antibiotic resistance profiles of Vibrio cholerae O139 in Shandong province.Methods A total of 13 strains of V.cholerae O139(9 clinical strains and 4 environmental strains)isolated from cholera epidemics in Shandong province since 1997 were recovered and confirmed with serum agglutination and biochemical reaction.Pulsed-field gel electrophoresis(PFGE)was carried out for molecular subtyping.Virulence genes

Effect of SiO_2 nanoparticles exposure on microRNA expression level in human bronchial epithelial cells

Objective To investigate the effect of short and long term exposure to Si O2nanoparticles on microRNA expression level in human bronchial epithelial cells(16HBE cells).Methods The 16HBE cells were exposed to 5,10,15,20,25,30 and 40μg/ml Si O2nanoparticles for24 h to detect the cell viability by using CCK-8 assay.

Prevalence of dental caries in fluorosis areas of Shantou City Guangdong Province after water improvement

Objective To analyse the relationship between children urine fluoride level,time of water supply improvement and detection rate of dental caries in fluorosis areas after water supply improvement.Methods The

The influence of microcystin-LR on monocytes and lymphocytes of mice

Objective To investigate the influence of microcystin-LR (MC-LR) on monocytes and lymphocytes in blood of mice and to find a sensitive index of toxic effects.Methods Specific pathogen free Kunming male mice,aging 1 month-old,were randomly divided into 5 groups

Oxidative stress and mitochondrion-related cell apoptosis in human bronchial epithelial 16HBE cells induced by silica dust

Objective To investigate oxidative stress and mitochondria-related cell apoptosis induced by silica dust in human normal bronchial epithelial 16HBE cells.Methods Cell viability,lactate dehydrogenase(LDH),superoxide dismutase(SOD),cell apoptotic rate,the expression level of Bax and Bcl-2 m RNA were measured

Levels of complement components C3a and C5a in renal injury among trichloroethylene-sensitized BALB/c mice

Objective To determine the levels of complement components C3a and C5a in the kidneys of trichloroethylene(TCE)-sensitized BALB/c mice,and to investigate the role of complement components in TCE-induced renal injury among BALB/c mice.Methods Sixty-two female BALB/c mice were randomly divided into blank control group,vehicle control group,and TCE

The effectiveness and safety of L-amlodipine besylate for blood pressure control in patients with mild to moderate essential hypertension

Objective To evaluate the effectiveness and safety of L-amlodipine besylate for blood pressure control in patients with mild to moderate essential hypertension.Methods A total of 1051 mild to moderate

The relationship between chemokine ligand 1 gene polymorphism and type 2 diabetes mellitus with pulmonary tuberculosis

Objective To explore the relationship between chemokine(CC motif)ligand1(CCL1)gene polymorphisms(rs159291,rs159294 and rs210837)and the susceptibility of type 2 diabetes mellitus with pulmonary tuberculosis(T2DM-PTB).Methods 124 T2DM cases,124T2DM-PTB cases and 130 healthy controls(NC)were collected in this case-control study.The genotypes

Analysis of the investigation results of iodine content in drinking water in Jiangsu Province in 2017

Objective To investigate the iodine content distribution of drinking water in Jiangsu Province.Methods In 2017,the iodine content in drinking water of residents in townships(streets,hereinafter referred as townships)of Jiangsu Province was investigated.Administrative villages(neighborhood committees,hereinafter referred as administrative villages)of Jiangsu Province were selected as units for the investigation of townships with the median water iodine greater than 10 pig/L.

Effects of staple food structure and self-regulation on blood glucose control in patients with type 2 diabetes mellitus

Objective To investigate the characteristics of staple food structure by using latent profile analysis in patients with type 2 diabetes mellitus(T2DM)and its relationship with blood glucose control and self-efficacy.

Investigation of the effect of occupational lead exposure on bone mineral density of workers

Objective To understand the occupational health status of a lead-acid battery enterprise in Jiangsu Province,to observe the results of blood lead and bone mineral density(BMD)of the workers exposed to occupa-tional lead,and to explore the effect of occupational lead exposure on BMD,so as to provide basis for the prevention and treatment of occupational lead poisoning and osteoporosis.