Objective To compare selenium content in hair samples of people in Kaschin-Beck disease(KBD)areas and non KBD areas two months after stopping selenium salt in Shaanxi Province,to provide a scientific basis for KBD con...Objective To compare selenium content in hair samples of people in Kaschin-Beck disease(KBD)areas and non KBD areas two months after stopping selenium salt in Shaanxi Province,to provide a scientific basis for KBD control and prevention.Methods In September2012,four historical KBD areas were selected,including Yongshou,Yuyang,Linyou and Nanzheng,four展开更多
Objective To analyze the differences in the composition and abundance of gut microbiota between patients with active pulmonary tuberculosis and healthy controls,and to identify the specific bacteria as biomarkers to d...Objective To analyze the differences in the composition and abundance of gut microbiota between patients with active pulmonary tuberculosis and healthy controls,and to identify the specific bacteria as biomarkers to distinguish between the two groups.Methods Patients with active pulmonary tuberculosis treated in three municipal designated tuberculosis medical institutions in Sichuan,Jiangsu and Shanghai from September 2017 to September 2019 were selected as case group(n=88),and the healthy people without a history of tuberculosis from the same regions were recruited as control group(n=62).展开更多
The onsite next generation sequencing(NGS)of Ebola virus(EBOV)genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin,transmission,and evolution of this virus.Herein,...The onsite next generation sequencing(NGS)of Ebola virus(EBOV)genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin,transmission,and evolution of this virus.Herein,we have diagnosed a cohort of EBOV patients in Sierra Leone in 2015,during the late phase of the outbreak.The surviving EBOV patients had a recovery process characterized by decreasing viremia,fever,and biochemical parameters.EBOV genomes sequenced through the longitudinal blood samples of these patients showed dynamic intra-host substitutions of the virus during acute infection,including the previously described short stretches of 13 serial TNC mutations.Remarkably,within individual patients,samples collected during the early phase of infection possessed Ts at these nucleotide sites,whereas they were replaced by Cs in samples collected in the later phase,suggesting that these short stretches of TNC mutations could emerge independently.In addition,up to a total of 35 nucleotide sites spanning the EBOV genome were mutated coincidently.Our study showed the dynamic intra-host adaptation of EBOV during patient recovery and gave more insight into the complex EBOV-host interactions.展开更多
Objective To observe the effects of fluoride on testicular cell cycle and cell apoptosis of male rats.Methods Thirty-two healthy male Wistar rats,weighting 150-180 g,were randomly divided into 4 groups by body weight ...Objective To observe the effects of fluoride on testicular cell cycle and cell apoptosis of male rats.Methods Thirty-two healthy male Wistar rats,weighting 150-180 g,were randomly divided into 4 groups by body weight using random number table,normal sodium(control),the low-dose,medium-dose and high-dose groups(100,200,300 mg·kg-1·d-1Na F,respectively)by intragastric administration for 90 days,and展开更多
Objective To explore the combined effects of fluoride and arsenite on the expression of Runx-related transcription 2(Runx2)mRNA in bone of Sprague Dawley(SD)rats.Methods Fifty four SD rats were selected[body mass(109....Objective To explore the combined effects of fluoride and arsenite on the expression of Runx-related transcription 2(Runx2)mRNA in bone of Sprague Dawley(SD)rats.Methods Fifty four SD rats were selected[body mass(109.71±10.52)g,half male and half female].3×3 Factorial experimental design was used to evaluate the combined effects of fluoride and arsenite on the expression of Runx2 mRNA by random number table.展开更多
Objective To explore the influence of fluorine on mRNA and protein expression of the insulin-like growth factor-1 (IGF-1) and its receptor of rat osteoblasts.Methods Osteoblasts were isolated from rat bone by enzyme d...Objective To explore the influence of fluorine on mRNA and protein expression of the insulin-like growth factor-1 (IGF-1) and its receptor of rat osteoblasts.Methods Osteoblasts were isolated from rat bone by enzyme digestion.Different fluorine concentration[0 (con-展开更多
Objective To explore the influence factors of saltsensitive hypertension and to observe changes of blood pressures and urinary sodium and potassium excretion in response to acute oral saline loading among essential hy...Objective To explore the influence factors of saltsensitive hypertension and to observe changes of blood pressures and urinary sodium and potassium excretion in response to acute oral saline loading among essential hypertensive patients in China.Methods Essential hypertensive patients from Beijing Jinzhan second community were included in this study.Salt-sensitivity was determined via the improved Sullivan’s acute oral saline展开更多
Objective To understand the current status of lung cancer disease burden in Jinchang cohort.Methods In this historical cohort study,the mortality data of the lung cancer from 2001 to 2013 and medical records of the lu...Objective To understand the current status of lung cancer disease burden in Jinchang cohort.Methods In this historical cohort study,the mortality data of the lung cancer from 2001 to 2013 and medical records of the lung caner cases from 2001 to 2010 in Jinchang cohort were used,analyze mortality,direct economic burden,poten-展开更多
Objective To understand the current status of disease burden caused by gastric cancer in Jinchang cohort.Methods In this historical cohort study,the data of gastric cancer deaths from 2001 to 2013 and the medical reco...Objective To understand the current status of disease burden caused by gastric cancer in Jinchang cohort.Methods In this historical cohort study,the data of gastric cancer deaths from 2001 to 2013 and the medical records of gastric cancer cases from 2001 to 2010 in Jinchang cohort were collected to analyze the mortality,po-展开更多
Objective To investigate the molecular epidemiological characteristics and antibiotic resistance profiles of Vibrio cholerae O139 in Shandong province.Methods A total of 13 strains of V.cholerae O139(9 clinical strain...Objective To investigate the molecular epidemiological characteristics and antibiotic resistance profiles of Vibrio cholerae O139 in Shandong province.Methods A total of 13 strains of V.cholerae O139(9 clinical strains and 4 environmental strains)isolated from cholera epidemics in Shandong province since 1997 were recovered and confirmed with serum agglutination and biochemical reaction.Pulsed-field gel electrophoresis(PFGE)was carried out for molecular subtyping.Virulence genes展开更多
Objective To investigate the effect of short and long term exposure to Si O2nanoparticles on microRNA expression level in human bronchial epithelial cells(16HBE cells).Methods The 16HBE cells were exposed to 5,10,15,2...Objective To investigate the effect of short and long term exposure to Si O2nanoparticles on microRNA expression level in human bronchial epithelial cells(16HBE cells).Methods The 16HBE cells were exposed to 5,10,15,20,25,30 and 40μg/ml Si O2nanoparticles for24 h to detect the cell viability by using CCK-8 assay.展开更多
Objective To analyse the relationship between children urine fluoride level,time of water supply improvement and detection rate of dental caries in fluorosis areas after water supply improvement.Methods The
Objective To investigate the influence of microcystin-LR (MC-LR) on monocytes and lymphocytes in blood of mice and to find a sensitive index of toxic effects.Methods Specific pathogen free Kunming male mice,aging 1 mo...Objective To investigate the influence of microcystin-LR (MC-LR) on monocytes and lymphocytes in blood of mice and to find a sensitive index of toxic effects.Methods Specific pathogen free Kunming male mice,aging 1 month-old,were randomly divided into 5 groups展开更多
Objective To investigate oxidative stress and mitochondria-related cell apoptosis induced by silica dust in human normal bronchial epithelial 16HBE cells.Methods Cell viability,lactate dehydrogenase(LDH),superoxide di...Objective To investigate oxidative stress and mitochondria-related cell apoptosis induced by silica dust in human normal bronchial epithelial 16HBE cells.Methods Cell viability,lactate dehydrogenase(LDH),superoxide dismutase(SOD),cell apoptotic rate,the expression level of Bax and Bcl-2 m RNA were measured展开更多
Objective To determine the levels of complement components C3a and C5a in the kidneys of trichloroethylene(TCE)-sensitized BALB/c mice,and to investigate the role of complement components in TCE-induced renal injury a...Objective To determine the levels of complement components C3a and C5a in the kidneys of trichloroethylene(TCE)-sensitized BALB/c mice,and to investigate the role of complement components in TCE-induced renal injury among BALB/c mice.Methods Sixty-two female BALB/c mice were randomly divided into blank control group,vehicle control group,and TCE展开更多
Objective To evaluate the effectiveness and safety of L-amlodipine besylate for blood pressure control in patients with mild to moderate essential hypertension.Methods A total of 1051 mild to moderate
Objective To explore the relationship between chemokine(CC motif)ligand1(CCL1)gene polymorphisms(rs159291,rs159294 and rs210837)and the susceptibility of type 2 diabetes mellitus with pulmonary tuberculosis(T2DM-PTB)....Objective To explore the relationship between chemokine(CC motif)ligand1(CCL1)gene polymorphisms(rs159291,rs159294 and rs210837)and the susceptibility of type 2 diabetes mellitus with pulmonary tuberculosis(T2DM-PTB).Methods 124 T2DM cases,124T2DM-PTB cases and 130 healthy controls(NC)were collected in this case-control study.The genotypes展开更多
Objective To investigate the iodine content distribution of drinking water in Jiangsu Province.Methods In 2017,the iodine content in drinking water of residents in townships(streets,hereinafter referred as townships)o...Objective To investigate the iodine content distribution of drinking water in Jiangsu Province.Methods In 2017,the iodine content in drinking water of residents in townships(streets,hereinafter referred as townships)of Jiangsu Province was investigated.Administrative villages(neighborhood committees,hereinafter referred as administrative villages)of Jiangsu Province were selected as units for the investigation of townships with the median water iodine greater than 10 pig/L.展开更多
Objective To investigate the characteristics of staple food structure by using latent profile analysis in patients with type 2 diabetes mellitus(T2DM)and its relationship with blood glucose control and self-efficacy.
Objective To understand the occupational health status of a lead-acid battery enterprise in Jiangsu Province,to observe the results of blood lead and bone mineral density(BMD)of the workers exposed to occupa-tional le...Objective To understand the occupational health status of a lead-acid battery enterprise in Jiangsu Province,to observe the results of blood lead and bone mineral density(BMD)of the workers exposed to occupa-tional lead,and to explore the effect of occupational lead exposure on BMD,so as to provide basis for the prevention and treatment of occupational lead poisoning and osteoporosis.展开更多
文摘Objective To compare selenium content in hair samples of people in Kaschin-Beck disease(KBD)areas and non KBD areas two months after stopping selenium salt in Shaanxi Province,to provide a scientific basis for KBD control and prevention.Methods In September2012,four historical KBD areas were selected,including Yongshou,Yuyang,Linyou and Nanzheng,four
文摘Objective To analyze the differences in the composition and abundance of gut microbiota between patients with active pulmonary tuberculosis and healthy controls,and to identify the specific bacteria as biomarkers to distinguish between the two groups.Methods Patients with active pulmonary tuberculosis treated in three municipal designated tuberculosis medical institutions in Sichuan,Jiangsu and Shanghai from September 2017 to September 2019 were selected as case group(n=88),and the healthy people without a history of tuberculosis from the same regions were recruited as control group(n=62).
基金supported by the Megaproject for Infectious Disease Research of China(2016ZX10004222-003)the research of Ebola pathogen from the National Natural Science Foundation of China(NSFC,81590763)+4 种基金National Key Research and Development Program of China(2016YFC1200200 to Y.Shu)the Distinguished Young Scientist Program of the NSFC(81525017 to Y.Shu)the Excellent Young Scientist Program of the NSFC(81822040 to W.J.Liu)the Taishan Scholar Project of Shandong Province(ts201511056 to W.Shi)G.F.Gao is a primary principal investigator of the NSFC Innovative Research Group(81621091).
文摘The onsite next generation sequencing(NGS)of Ebola virus(EBOV)genomes during the 2013–2016 Ebola epidemic in Western Africa provides an opportunity to trace the origin,transmission,and evolution of this virus.Herein,we have diagnosed a cohort of EBOV patients in Sierra Leone in 2015,during the late phase of the outbreak.The surviving EBOV patients had a recovery process characterized by decreasing viremia,fever,and biochemical parameters.EBOV genomes sequenced through the longitudinal blood samples of these patients showed dynamic intra-host substitutions of the virus during acute infection,including the previously described short stretches of 13 serial TNC mutations.Remarkably,within individual patients,samples collected during the early phase of infection possessed Ts at these nucleotide sites,whereas they were replaced by Cs in samples collected in the later phase,suggesting that these short stretches of TNC mutations could emerge independently.In addition,up to a total of 35 nucleotide sites spanning the EBOV genome were mutated coincidently.Our study showed the dynamic intra-host adaptation of EBOV during patient recovery and gave more insight into the complex EBOV-host interactions.
文摘Objective To observe the effects of fluoride on testicular cell cycle and cell apoptosis of male rats.Methods Thirty-two healthy male Wistar rats,weighting 150-180 g,were randomly divided into 4 groups by body weight using random number table,normal sodium(control),the low-dose,medium-dose and high-dose groups(100,200,300 mg·kg-1·d-1Na F,respectively)by intragastric administration for 90 days,and
文摘Objective To explore the combined effects of fluoride and arsenite on the expression of Runx-related transcription 2(Runx2)mRNA in bone of Sprague Dawley(SD)rats.Methods Fifty four SD rats were selected[body mass(109.71±10.52)g,half male and half female].3×3 Factorial experimental design was used to evaluate the combined effects of fluoride and arsenite on the expression of Runx2 mRNA by random number table.
文摘Objective To explore the influence of fluorine on mRNA and protein expression of the insulin-like growth factor-1 (IGF-1) and its receptor of rat osteoblasts.Methods Osteoblasts were isolated from rat bone by enzyme digestion.Different fluorine concentration[0 (con-
文摘Objective To explore the influence factors of saltsensitive hypertension and to observe changes of blood pressures and urinary sodium and potassium excretion in response to acute oral saline loading among essential hypertensive patients in China.Methods Essential hypertensive patients from Beijing Jinzhan second community were included in this study.Salt-sensitivity was determined via the improved Sullivan’s acute oral saline
文摘Objective To understand the current status of lung cancer disease burden in Jinchang cohort.Methods In this historical cohort study,the mortality data of the lung cancer from 2001 to 2013 and medical records of the lung caner cases from 2001 to 2010 in Jinchang cohort were used,analyze mortality,direct economic burden,poten-
文摘Objective To understand the current status of disease burden caused by gastric cancer in Jinchang cohort.Methods In this historical cohort study,the data of gastric cancer deaths from 2001 to 2013 and the medical records of gastric cancer cases from 2001 to 2010 in Jinchang cohort were collected to analyze the mortality,po-
文摘Objective To investigate the molecular epidemiological characteristics and antibiotic resistance profiles of Vibrio cholerae O139 in Shandong province.Methods A total of 13 strains of V.cholerae O139(9 clinical strains and 4 environmental strains)isolated from cholera epidemics in Shandong province since 1997 were recovered and confirmed with serum agglutination and biochemical reaction.Pulsed-field gel electrophoresis(PFGE)was carried out for molecular subtyping.Virulence genes
文摘Objective To investigate the effect of short and long term exposure to Si O2nanoparticles on microRNA expression level in human bronchial epithelial cells(16HBE cells).Methods The 16HBE cells were exposed to 5,10,15,20,25,30 and 40μg/ml Si O2nanoparticles for24 h to detect the cell viability by using CCK-8 assay.
文摘Objective To analyse the relationship between children urine fluoride level,time of water supply improvement and detection rate of dental caries in fluorosis areas after water supply improvement.Methods The
文摘Objective To investigate the influence of microcystin-LR (MC-LR) on monocytes and lymphocytes in blood of mice and to find a sensitive index of toxic effects.Methods Specific pathogen free Kunming male mice,aging 1 month-old,were randomly divided into 5 groups
文摘Objective To investigate oxidative stress and mitochondria-related cell apoptosis induced by silica dust in human normal bronchial epithelial 16HBE cells.Methods Cell viability,lactate dehydrogenase(LDH),superoxide dismutase(SOD),cell apoptotic rate,the expression level of Bax and Bcl-2 m RNA were measured
文摘Objective To determine the levels of complement components C3a and C5a in the kidneys of trichloroethylene(TCE)-sensitized BALB/c mice,and to investigate the role of complement components in TCE-induced renal injury among BALB/c mice.Methods Sixty-two female BALB/c mice were randomly divided into blank control group,vehicle control group,and TCE
文摘Objective To evaluate the effectiveness and safety of L-amlodipine besylate for blood pressure control in patients with mild to moderate essential hypertension.Methods A total of 1051 mild to moderate
文摘Objective To explore the relationship between chemokine(CC motif)ligand1(CCL1)gene polymorphisms(rs159291,rs159294 and rs210837)and the susceptibility of type 2 diabetes mellitus with pulmonary tuberculosis(T2DM-PTB).Methods 124 T2DM cases,124T2DM-PTB cases and 130 healthy controls(NC)were collected in this case-control study.The genotypes
文摘Objective To investigate the iodine content distribution of drinking water in Jiangsu Province.Methods In 2017,the iodine content in drinking water of residents in townships(streets,hereinafter referred as townships)of Jiangsu Province was investigated.Administrative villages(neighborhood committees,hereinafter referred as administrative villages)of Jiangsu Province were selected as units for the investigation of townships with the median water iodine greater than 10 pig/L.
文摘Objective To investigate the characteristics of staple food structure by using latent profile analysis in patients with type 2 diabetes mellitus(T2DM)and its relationship with blood glucose control and self-efficacy.
文摘Objective To understand the occupational health status of a lead-acid battery enterprise in Jiangsu Province,to observe the results of blood lead and bone mineral density(BMD)of the workers exposed to occupa-tional lead,and to explore the effect of occupational lead exposure on BMD,so as to provide basis for the prevention and treatment of occupational lead poisoning and osteoporosis.