Early results of the integrative epigenomic-transcriptomic landscape of colorectal adenoma and cancer

BACKGROUND Aberrant methylation is common during the initiation and progression of colorectal cancer(CRC),and detecting these changes that occur during early adenoma(ADE)formation and CRC progression has clinical value.AIM To identify potential DNA methylation markers specific to ADE and CRC.METHODS Here,we performed SeqCap targeted bisulfite sequencing and RNA-seq analysis of colorectal ADE and CRC samples to profile the epigenomic-transcriptomic landscape.RESULTS Comparing 22 CRC and 25 ADE samples,global methylation was higher in the former,but both showed similar methylation patterns regarding differentially methylated gene positions,chromatin signatures,and repeated elements.High-grade CRC tended to exhibit elevated methylation levels in gene promoter regions compared to those in low-grade CRC.Combined with RNA-seq gene expression data,we identified 14 methylation-regulated differentially expressed genes,of which only AGTR1 and NECAB1 methylation had prognostic significance.CONCLUSION Our results suggest that genome-wide alterations in DNA methylation occur during the early stages of CRC and demonstrate the methylation signatures associated with colorectal ADEs and CRC,suggesting prognostic biomarkers for CRC.

Identification of a novel mtDNA lineage B3 in chicken (Gallus gallus domesticus)

DEAR EDITOR,In this study,we sequenced the complete mitochondrial DNA genome(mitogenome)of the Zhengyang Yellow chicken(Gallus gallus domesticus)by next-generation sequencing technology.Samples were taken from Zhumadian city,Henan Province,China.The complete mitogenome was 16 785 bp

Genome of Plant Maca （Lepidium meyenil） Illuminates Genomic Basis for High-Altitude Adaptation in the Central Andes

Maca (Lepidium meyenii Walp， 2n = 8x = 64 ) ，属于 Brassicaceae 家庭，经济植物在秘鲁(4000-4500 m ) 在中央安第斯山脉岭被栽培。认为快速中央安第斯山脉高举发生 5-10 百万年以前(妈) ，一个进化问题关于象 maca 那样的植物怎么在一个短地质的时期以内获得高高度的改编产生。这里，我们报导 maca 的高质量的染色体集会，在里面它二仔细 spaced maca 特定的整个染色体的复制(WGD；6.7 妈) 被识别。在 maca 和密切相关的 Brassicaceae 种类之间的比较 genomic 分析揭示了经由 WGD 涉及不能生活的压力反应，荷尔蒙发信号小径，和第二等的代谢物生合成的 maca 基因和基因家庭的扩大。保留和许多复制基因的随后的功能的分叉可以说明词法、生理的变化(即，小叶形状和自我富饶) 在在高高度的环境的 maca。另外，一些复制 maca 基因在词法改编与功能被识别(即，叶卷应答) 并且不能生活的压力反应(即， GLYCINE 富有的 RNA 有约束力的蛋白质和 DNA-DAMAGE-REPAIR/TOLERATION 2 ) 在积极选择下面。一起， maca 染色体提供有用信息在安第斯山脉在植物的高高度的改编理解 WGD 的重要角色。

Cytotoxic germacrane-type sesquiterpene lactones from the whole plant of Inula cappa

Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E(1 and 2),together with three known analogs.The structures of the new compounds were established by extensive analysis of 1Dand 2DNMR spectra,as well as MS data.Their absolute configurations were determined by CD spectra.All compounds showed moderate inhibitory effects on A431,A549,BGC-823,HL-60,HT-29,and MCF-7 cancer cell lines with IC_(50) values ranging from 2.1 to 36.3 μM.

Concealed Wireless Warning Sensor Based on Triboelectrification and Human-Plant Interactive Induction

With the continuous development of artificial intelligence,the demand for sensors with simple preparation and strong concealment continues to increase.However,most of the high-sensitivity sensors have complex manufacturing methods,high costs,and single functions.In this paper,a sensitive motion sensor based on the triboelectric interaction between a living plant and the human body was designed to detect the real-time movements of human beings and provide danger warning.A certain relationship exists between the triboelectric signal and the distance between the plant and the human body,with effective signals being detected in the range of 1.8 m.In addition,the triboelectric signal generated by each person is unique like a fingerprint,which can be used for biometrics.On the basis of the triboelectric signal,a wireless character entry warning system is designed.This sensor can not only send out a wireless warning signal at a specific distance but also allow one to receive the warning information synchronously on a mobile phone in real time.The wireless movement sensor receives signals through a living plant,and it has the characteristics of convenient use,strong concealment,and shielding difficulty.This sensor has the potential to be widely used in person recognition,danger warning,and motion monitoring.

Longitudinal transcriptome analyses show robust T cell immunity during recovery from COVID-19

Understanding the processes of immune regulation in patients infected with the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is crucial for improving treatment.Here,we performed longitudinal whole-transcriptome RNA sequencing on peripheral blood mononuclear cell(PBMC)samples from 18 patients with coronavirus disease 2019(COVID-19)during their treatment,convalescence,and rehabilitation.After analyzing the regulatory networks of differentially expressed messenger RNAs(mRNAs),microRNAs(miRNAs)and long non-coding RNAs(lncRNAs)between the different clinical stages,we found that humoral immunity and type I interferon response were significantly downregulated,while robust T-cell activation and differentiation at the whole transcriptome level constituted the main events that occurred during recovery from COVID-19.The formation of this T cell immune response might be driven by the activation of activating protein-1(AP-1)related signaling pathway and was weakly affected by other clinical features.These findings uncovered the dynamic pattern of immune responses and indicated the key role of T cell immunity in the creation of immune protection against this disease.

Longitudinal transcriptome analyses show robust T cellimmunity during recovery from COVID-19

Understanding the processes of immune regulation in patients infected with the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is crucial for improving treatment.Here,we performed longitudinal whole-transcriptome RNA sequencing on peripheral blood mononuclear cell(PBMC)samples from 18 patients with coronavirus disease 2019(COVID-19)during their treatment,convalescence,and rehabilitation.After analyzing the regulatory networks of differentially expressed messenger RNAs(mRNAs),microRNAs(miRNAs)and long non-coding RNAs(lncRNAs)between the different clinical stages,we found that humoral immunity and type I interferon response were significantly downregulated,while robust T-cell activation and differentiation at the whole transcriptome level constituted the main events that occurred during recovery from COVID-19.The formation of this T cell immune response might be driven by the activation of activating protein-1(AP-1)related signaling pathway and was weakly affected by other clinical features.These findings uncovered the dynamic pattern of immune responses and indicated the key role of T cell immunity in the creation of immune protection against this disease.