Chinese sprangletop (Leptochloa chinensis), belonging to the grass subfamily Chloridoideae, is one of the most notorious weeds in rice ecosystems. Here, we report a chromosome-scale reference genome assembly and a gen...Chinese sprangletop (Leptochloa chinensis), belonging to the grass subfamily Chloridoideae, is one of the most notorious weeds in rice ecosystems. Here, we report a chromosome-scale reference genome assembly and a genomic variation map of the tetraploid L. chinensis. The L. chinensis genome is derived from two diploid progenitors that diverged ∼10.9 million years ago, and its two subgenomes display neither fractionation bias nor overall gene expression dominance. Comparative genomic analyses reveal substantial genome rearrangements in L. chinensis after its divergence from the common ancestor of Chloridoideae and, together with transcriptome profiling, demonstrate the important contribution of tetraploidization to the gene sources for the herbicide resistance of L. chinensis. Population genomic analyses of 89 accessions from China reveal that L. chinensis accessions collected from southern/southwestern provinces have substantially higher nucleotide diversity than those from the middle and lower reaches of the Yangtze River, suggesting that L. chinensis spread in China from the southern/southwestern provinces to the middle and lower reaches of the Yangtze River. During this spread, L. chinensis developed significantly increased herbicide resistance, accompanied by the selection of numerous genes involved in herbicide resistance. Taken together, our study generated valuable genomic resources for future fundamental research and agricultural management of L. chinensis, and provides significant new insights into the herbicide resistance as well as the origin and adaptive evolution of L. chinensis.展开更多
Dear Editors,CRISPR/Cas9-mediated genome editing techniques have triggered a revolution in biology research(Jinek et al.,2012).Cas9 coupled with guide RNAs cuts DNA at precise positions,and the resulting double strand...Dear Editors,CRISPR/Cas9-mediated genome editing techniques have triggered a revolution in biology research(Jinek et al.,2012).Cas9 coupled with guide RNAs cuts DNA at precise positions,and the resulting double stranded breaks(DSB)were effectively repaired by non-homologous end joining(NHEJ)pathway in higher eukaryotic cells,including animals and plants.展开更多
基金supported by grants from the National Key R&D Program of China(No.2021YFD1700101)the National Natural Science Foundation of China(No.32130091 and No.32001923)+2 种基金the science And and Technology Innovation Program of Hunan Province (No.2020WK2014 and No.2020WK2023)the Training Program for Excellent Young Innovators of Changsha(kg2106079)the China Agriculture Research System of MOF and MARA(CARS-16-E19)。
文摘Chinese sprangletop (Leptochloa chinensis), belonging to the grass subfamily Chloridoideae, is one of the most notorious weeds in rice ecosystems. Here, we report a chromosome-scale reference genome assembly and a genomic variation map of the tetraploid L. chinensis. The L. chinensis genome is derived from two diploid progenitors that diverged ∼10.9 million years ago, and its two subgenomes display neither fractionation bias nor overall gene expression dominance. Comparative genomic analyses reveal substantial genome rearrangements in L. chinensis after its divergence from the common ancestor of Chloridoideae and, together with transcriptome profiling, demonstrate the important contribution of tetraploidization to the gene sources for the herbicide resistance of L. chinensis. Population genomic analyses of 89 accessions from China reveal that L. chinensis accessions collected from southern/southwestern provinces have substantially higher nucleotide diversity than those from the middle and lower reaches of the Yangtze River, suggesting that L. chinensis spread in China from the southern/southwestern provinces to the middle and lower reaches of the Yangtze River. During this spread, L. chinensis developed significantly increased herbicide resistance, accompanied by the selection of numerous genes involved in herbicide resistance. Taken together, our study generated valuable genomic resources for future fundamental research and agricultural management of L. chinensis, and provides significant new insights into the herbicide resistance as well as the origin and adaptive evolution of L. chinensis.
基金supported by the National Transgenic Science and Technology Program(2019ZX08010-003)National Natural Science Foundation of China(31872933)。
文摘Dear Editors,CRISPR/Cas9-mediated genome editing techniques have triggered a revolution in biology research(Jinek et al.,2012).Cas9 coupled with guide RNAs cuts DNA at precise positions,and the resulting double stranded breaks(DSB)were effectively repaired by non-homologous end joining(NHEJ)pathway in higher eukaryotic cells,including animals and plants.
