[Objectives]This study was conducted for the purpose of widely using molecular biology techniques in the detection of seafood species.[Methods]In this study,such four kinds of seafood as fish,shrimps,crabs and shellfi...[Objectives]This study was conducted for the purpose of widely using molecular biology techniques in the detection of seafood species.[Methods]In this study,such four kinds of seafood as fish,shrimps,crabs and shellfish were used as samples to compare three DNA extraction methods,including Tiangen marine animal tissue genome extraction kit method(DP324)(a),SN/T 3589.6-2013/8.3(b)method and modified SN/T 3730.8-2013/8.1(c)method.The purity and concentrations of DNA and the amplification effects of real-time fluorescene PCR were compared.[Results]The three methods had different extraction effects while satisfying the real-time fluorescent PCR detection.The DNA templates extracted by the SN/T 3730.8-2013/8.1 standard method had higher purity and concentrations,and showed clearer bands in gel electrophoresis,indicating that the method has less effect on real-time fluorescent PCR and less inhibition,and can meet the detection needs of the four types of seafood,including fish,shrimps,crabs and shellfish.[Conclusions]This study provides a theoretical basis for the detection of seafood animal-derived components.展开更多
文摘[Objectives]This study was conducted for the purpose of widely using molecular biology techniques in the detection of seafood species.[Methods]In this study,such four kinds of seafood as fish,shrimps,crabs and shellfish were used as samples to compare three DNA extraction methods,including Tiangen marine animal tissue genome extraction kit method(DP324)(a),SN/T 3589.6-2013/8.3(b)method and modified SN/T 3730.8-2013/8.1(c)method.The purity and concentrations of DNA and the amplification effects of real-time fluorescene PCR were compared.[Results]The three methods had different extraction effects while satisfying the real-time fluorescent PCR detection.The DNA templates extracted by the SN/T 3730.8-2013/8.1 standard method had higher purity and concentrations,and showed clearer bands in gel electrophoresis,indicating that the method has less effect on real-time fluorescent PCR and less inhibition,and can meet the detection needs of the four types of seafood,including fish,shrimps,crabs and shellfish.[Conclusions]This study provides a theoretical basis for the detection of seafood animal-derived components.
