[Objective]The paper was to analyze whether Asiatic black bear( Ursus thibetanus) had different blood group systems. [Method]Whole blood samples were collected from 40 Asiatic black bears in Fujian Province,China. Tub...[Objective]The paper was to analyze whether Asiatic black bear( Ursus thibetanus) had different blood group systems. [Method]Whole blood samples were collected from 40 Asiatic black bears in Fujian Province,China. Tube method was used to detect antibodies in plasma,and antibody isotype was determined with 2-mercaptoethanol. Plasma was further analyzed by mass spectrometry. [Result] The plasma from four black bears had antibodies,possibly Ig M isoform,which could agglutinate RBCs from 30 bears. Blood samples from 10 bears were tested by human blood typing reagents. The results showed that four black bears had blood type like human type O,while six bears had like human type B. Mass spectrometry results demonstrated that plasma protein had extensive homology to serum albumin-like isoform 1 found in giant panda( Ailuropoda melanoleuca). [Conclusion]Asiatic black bear might have at least one blood group system with two blood types. If the sick bear needs blood transfusion,a cross-matching test is necessary. Moreover,giant panda might receive blood from Asiatic black bear in case of emergency.展开更多
基金Supported by Hong Kong Ocean Park Conservation Foundation(GP01-1617)Special Fund of Health Industry Research(201002005)
文摘[Objective]The paper was to analyze whether Asiatic black bear( Ursus thibetanus) had different blood group systems. [Method]Whole blood samples were collected from 40 Asiatic black bears in Fujian Province,China. Tube method was used to detect antibodies in plasma,and antibody isotype was determined with 2-mercaptoethanol. Plasma was further analyzed by mass spectrometry. [Result] The plasma from four black bears had antibodies,possibly Ig M isoform,which could agglutinate RBCs from 30 bears. Blood samples from 10 bears were tested by human blood typing reagents. The results showed that four black bears had blood type like human type O,while six bears had like human type B. Mass spectrometry results demonstrated that plasma protein had extensive homology to serum albumin-like isoform 1 found in giant panda( Ailuropoda melanoleuca). [Conclusion]Asiatic black bear might have at least one blood group system with two blood types. If the sick bear needs blood transfusion,a cross-matching test is necessary. Moreover,giant panda might receive blood from Asiatic black bear in case of emergency.
