Cell rotation is one of the most important techniques for cell manipulation in modern bioscience,as it not only permits cell observation from any arbitrary angle,but also simplifies the procedures for analyzing the me...Cell rotation is one of the most important techniques for cell manipulation in modern bioscience,as it not only permits cell observation from any arbitrary angle,but also simplifies the procedures for analyzing the mechanical properties of cells,characterizing cell physiology,and performing microsurgery.Numerous approaches have been reported for rotating cells in a wide range of academic and industrial applications.Among them,the most popular are micro-robot-based direct contact manipulation and field-based non-contact methods(e.g.,optical,magnetic,electric,acoustic,and hydrodynamic methods).This review first summarizes the fundamental mechanisms,merits,and demerits of these six main groups of approaches,and then discusses their differences and limitations in detail.We aim to bridge the gap between each method and illustrate the development progress,current advances,and prospects in the field of cell rotation.展开更多
Osteoarthritis(OA)is the main cause of disability in the elderly.Effective intervention in the early and middle stage of osteoarthritis can greatly prevent or slow down the development of the disease,and reduce the pr...Osteoarthritis(OA)is the main cause of disability in the elderly.Effective intervention in the early and middle stage of osteoarthritis can greatly prevent or slow down the development of the disease,and reduce the probability of joint replacement.However,there is to date no effective intervention for early and middle-stage OA.OA microenvironment mainly destroys the balance of oxidative stress,extracellular matrix synthesis and degradation of chondrocytes under the joint action of biological and mechanical factors.Herein,hollow Prussian blue nanozymes(HPBzymes)were designed via a modified hydrothermal template-free method.The aim of this study was to investigate the effects of HPBzymes on chondrocytes and the progression of OA.The intrinsic bioactivities of HPBzymes were excavated in vitro and in vivo,remodeling microenvironment for significantly protecting chondrocytes and delaying the progression of traumatic OA by inhibiting reactive oxygen species(ROS)and Rac1/nuclear factor kappa-B(NF-κB)signaling in a rat model.HPBzyme significantly diminished interleukin(IL)-1β-stimulated inflammation,extracellular matrix degradation,and apoptosis of human chondrocytes.HPBzyme attenuated the expression of Rac1 and the ROS levels and prevented the release and nuclear translocation of NF-κB.Deeply digging the intrinsic bioactivities of nanozyme with single component to remodel microenvironment is an effective strategy for ROS-associated chronic diseases.This study reveals that excavating the bioactivities of nanomedicine deserves attention for diagnosis and treatment of severe diseases.展开更多
Although the function of tRNAs in the translational process is well established,it remains controversial whether tRNA abundance is tightly associated with translational efficiency(TE)in mammals.Moreover,how critically...Although the function of tRNAs in the translational process is well established,it remains controversial whether tRNA abundance is tightly associated with translational efficiency(TE)in mammals.Moreover,how critically the expression of tRNAs contributes to the establishment of tissue-specific proteomes in mammals has not been well addressed.Here,we measured both tRNA expression using demethylase-tRNA sequencing(DM-tRNA-seq)and TE of mRNAs using ribosome-tagging sequencing(RiboTag-seq)in the brain,heart,and testis of mice.Remarkable variation in the expression of tRNA isodecoders was observed among different tissues.When the statistical effect of isodecoder-grouping on reducing variations is considered through permutating the anticodons,we observed an expected reduction in the variation of anticodon expression across all samples,an unexpected smaller variation of anticodon usage bias,and an unexpected larger variation of tRNA isotype expression at amino acid level.Regardless of whether or not they share the same anticodons,the isodecoders encoding the same amino acids are co-expressed across different tissues.Based on the expression of tRNAs and the TE of mRNAs,we find that the tRNA adaptation index(tAI)and TE are significantly correlated in the same tissues but not between tissues;and tRNA expression and the amino acid composition of translating peptides are positively correlated in the same tissues but not between tissues.We therefore hypothesize that the tissue-specific expression of tRNAs might be due to post-transcriptional mechanisms.This study provides a resource for tRNA and translation studies,as well as novel insights into the dynamics of tRNAs and their roles in translational regulation.展开更多
Alzheimer’s disease(AD)is the main cause of dementia,with its diagnosis and management remaining challenging.Amyloid positron emission tomography(PET)has become increasingly important in medical practice for patients...Alzheimer’s disease(AD)is the main cause of dementia,with its diagnosis and management remaining challenging.Amyloid positron emission tomography(PET)has become increasingly important in medical practice for patients with AD.To integrate and update previous guidelines in the field,a task group of experts of several disciplines from multiple countries was assembled,and they revised and approved the content related to the application of amyloid PET in the medical settings of cognitively impaired individuals,focusing on clinical scenarios,patient preparation,administered activities,as well as image acquisition,processing,interpretation and reporting.In addition,expert opinions,practices,and protocols of prominent research institutions performing research on amyloid PET of dementia are integrated.With the increasing availability of amyloid PET imaging,a complete and standard pipeline for the entire examination process is essential for clinical practice.This international consensus and practice guideline will help to promote proper clinical use of amyloid PET imaging in patients with AD.展开更多
Autism spectrum disorder(ASD)is a heterogeneous neurodevelopmental disorder associated with both genetic and environmental risks.Neuroimaging approaches have been widely employed to parse the neurophysiological mechan...Autism spectrum disorder(ASD)is a heterogeneous neurodevelopmental disorder associated with both genetic and environmental risks.Neuroimaging approaches have been widely employed to parse the neurophysiological mechanisms underlying ASD,and provide critical insights into the anatomical,functional,and neurochemical changes.We reviewed recent advances in neuroimaging studies that focused on ASD by using magnetic resonance imaging(MRI),positron emission tomography(PET),or single-positron emission tomography(SPECT).Longitudinal structural MRI has delineated an abnormal developmental trajectory of ASD that is associated with cascading neurobiological processes,and functional MRI has pointed to disrupted functional neural networks.Meanwhile,PET and SPECT imaging have revealed that metabolic and neurotransmitter abnormalities may contribute to shaping the aberrant neural circuits of ASD.Future large-scale,multi-center,multimodal investigations are essential to elucidate the neurophysiological underpinnings of ASD,and facilitate the development of novel diagnostic biomarkers and better-targeted therapy.展开更多
Tumors are complex ecosystems in which heterogeneous cancer cells interact with their microenvironment composed of diverse immune,endothelial,and stromal cells.Cancer biology had been studied using bulk genomic and ge...Tumors are complex ecosystems in which heterogeneous cancer cells interact with their microenvironment composed of diverse immune,endothelial,and stromal cells.Cancer biology had been studied using bulk genomic and gene expression profiling,which however mask the cellular diversity and average the variability among individual molecular programs.Recent advances in single-cell transcriptomic sequencing have enabled a detailed dissection of tumor ecosystems and promoted our understanding of tumorigenesis at single-cell resolution.In the present review,we discuss the main topics of recent cancer studies that have implemented singlecell RNA sequencing(scRNA-seq).To study cancer cells,scRNA-seq has provided novel insights into the cancer stem-cell model,treatment resistance,and cancer metastasis.To study the tumor microenvironment,scRNA-seq has portrayed the diverse cell types and complex cellular states of both immune and non-immune cells interacting with cancer cells,with the promise to discover novel targets for future immunotherapy.展开更多
基金supported by JSPS Grant-in-Aid for Scientific Research(20K15151)Australian Research Council Discovery Projects(DP200102269)+2 种基金JSPS Core-to-Core programAmada FoundationWhite Rock Foundation。
文摘Cell rotation is one of the most important techniques for cell manipulation in modern bioscience,as it not only permits cell observation from any arbitrary angle,but also simplifies the procedures for analyzing the mechanical properties of cells,characterizing cell physiology,and performing microsurgery.Numerous approaches have been reported for rotating cells in a wide range of academic and industrial applications.Among them,the most popular are micro-robot-based direct contact manipulation and field-based non-contact methods(e.g.,optical,magnetic,electric,acoustic,and hydrodynamic methods).This review first summarizes the fundamental mechanisms,merits,and demerits of these six main groups of approaches,and then discusses their differences and limitations in detail.We aim to bridge the gap between each method and illustrate the development progress,current advances,and prospects in the field of cell rotation.
基金supported by the National Natural Science Foundation of China(no.81801822)Shanghai S&T Major project(2018SHZDZX05)+3 种基金NSFC Key Projects of International Cooperation and Exchanges(81720108023)Shanghai Collaborative Innovation Center for Translational Medicine(no.TM201724)Natural Science Foundation of Shanghai(18ZR1429300)Interdisciplinary Program of Shanghai Jiao Tong University(no.YG2017QN20).
文摘Osteoarthritis(OA)is the main cause of disability in the elderly.Effective intervention in the early and middle stage of osteoarthritis can greatly prevent or slow down the development of the disease,and reduce the probability of joint replacement.However,there is to date no effective intervention for early and middle-stage OA.OA microenvironment mainly destroys the balance of oxidative stress,extracellular matrix synthesis and degradation of chondrocytes under the joint action of biological and mechanical factors.Herein,hollow Prussian blue nanozymes(HPBzymes)were designed via a modified hydrothermal template-free method.The aim of this study was to investigate the effects of HPBzymes on chondrocytes and the progression of OA.The intrinsic bioactivities of HPBzymes were excavated in vitro and in vivo,remodeling microenvironment for significantly protecting chondrocytes and delaying the progression of traumatic OA by inhibiting reactive oxygen species(ROS)and Rac1/nuclear factor kappa-B(NF-κB)signaling in a rat model.HPBzyme significantly diminished interleukin(IL)-1β-stimulated inflammation,extracellular matrix degradation,and apoptosis of human chondrocytes.HPBzyme attenuated the expression of Rac1 and the ROS levels and prevented the release and nuclear translocation of NF-κB.Deeply digging the intrinsic bioactivities of nanozyme with single component to remodel microenvironment is an effective strategy for ROS-associated chronic diseases.This study reveals that excavating the bioactivities of nanomedicine deserves attention for diagnosis and treatment of severe diseases.
基金supported by the National Key R&D Program of China(Grant No.2018YFA0107200 to Jinkai Wang)the National Natural Science Foundation of China(Grant Nos.31970594 and 31771446 to Jinkai Wang,Grant Nos.81922052 and 81974435 to Shuibin Lin+3 种基金Grant No.31971335 to Dan Ohtan Wang)the Natural Science Foundation of Guangdong,China(Grant No.2019B151502011 to Shuibin LinGrant No.2021A1515110650 to Yan Gao)the China Postdoctoral Science Foundation(Grant No.2021M703755 to Yan Gao).
文摘Although the function of tRNAs in the translational process is well established,it remains controversial whether tRNA abundance is tightly associated with translational efficiency(TE)in mammals.Moreover,how critically the expression of tRNAs contributes to the establishment of tissue-specific proteomes in mammals has not been well addressed.Here,we measured both tRNA expression using demethylase-tRNA sequencing(DM-tRNA-seq)and TE of mRNAs using ribosome-tagging sequencing(RiboTag-seq)in the brain,heart,and testis of mice.Remarkable variation in the expression of tRNA isodecoders was observed among different tissues.When the statistical effect of isodecoder-grouping on reducing variations is considered through permutating the anticodons,we observed an expected reduction in the variation of anticodon expression across all samples,an unexpected smaller variation of anticodon usage bias,and an unexpected larger variation of tRNA isotype expression at amino acid level.Regardless of whether or not they share the same anticodons,the isodecoders encoding the same amino acids are co-expressed across different tissues.Based on the expression of tRNAs and the TE of mRNAs,we find that the tRNA adaptation index(tAI)and TE are significantly correlated in the same tissues but not between tissues;and tRNA expression and the amino acid composition of translating peptides are positively correlated in the same tissues but not between tissues.We therefore hypothesize that the tissue-specific expression of tRNAs might be due to post-transcriptional mechanisms.This study provides a resource for tRNA and translation studies,as well as novel insights into the dynamics of tRNAs and their roles in translational regulation.
基金This study was partially sponsored by the National Natural Science Foundation of China(NSFC)(81761148029,81725009,82030049,82021002,81971641)Ministry of Science and Technology of China(MOST)(2021YFA110004500,2021YFE0108300)+3 种基金Clinical Research Plan of SHDC(SHDC2020CR1038B)Science and Technology Innovation 2030 Major Projects(2022ZD0211600)Swiss National Science Foundation(project nos.185028,188355,and 169876)National Research Foundation of Korea(NRF-2017K2A9A2A10013554).
文摘Alzheimer’s disease(AD)is the main cause of dementia,with its diagnosis and management remaining challenging.Amyloid positron emission tomography(PET)has become increasingly important in medical practice for patients with AD.To integrate and update previous guidelines in the field,a task group of experts of several disciplines from multiple countries was assembled,and they revised and approved the content related to the application of amyloid PET in the medical settings of cognitively impaired individuals,focusing on clinical scenarios,patient preparation,administered activities,as well as image acquisition,processing,interpretation and reporting.In addition,expert opinions,practices,and protocols of prominent research institutions performing research on amyloid PET of dementia are integrated.With the increasing availability of amyloid PET imaging,a complete and standard pipeline for the entire examination process is essential for clinical practice.This international consensus and practice guideline will help to promote proper clinical use of amyloid PET imaging in patients with AD.
基金the National Key Research and the Development Program of China(2016YFA0100900)the National Natural Science Foundation of China(81761148029,81725009,81425015,and 81900255).
文摘Autism spectrum disorder(ASD)is a heterogeneous neurodevelopmental disorder associated with both genetic and environmental risks.Neuroimaging approaches have been widely employed to parse the neurophysiological mechanisms underlying ASD,and provide critical insights into the anatomical,functional,and neurochemical changes.We reviewed recent advances in neuroimaging studies that focused on ASD by using magnetic resonance imaging(MRI),positron emission tomography(PET),or single-positron emission tomography(SPECT).Longitudinal structural MRI has delineated an abnormal developmental trajectory of ASD that is associated with cascading neurobiological processes,and functional MRI has pointed to disrupted functional neural networks.Meanwhile,PET and SPECT imaging have revealed that metabolic and neurotransmitter abnormalities may contribute to shaping the aberrant neural circuits of ASD.Future large-scale,multi-center,multimodal investigations are essential to elucidate the neurophysiological underpinnings of ASD,and facilitate the development of novel diagnostic biomarkers and better-targeted therapy.
基金This work was financially supported by the National Science and Technology Major Project(2019YFC1315702)the Guangdong Province Key Research and Development Program(2019B020226002).
文摘Tumors are complex ecosystems in which heterogeneous cancer cells interact with their microenvironment composed of diverse immune,endothelial,and stromal cells.Cancer biology had been studied using bulk genomic and gene expression profiling,which however mask the cellular diversity and average the variability among individual molecular programs.Recent advances in single-cell transcriptomic sequencing have enabled a detailed dissection of tumor ecosystems and promoted our understanding of tumorigenesis at single-cell resolution.In the present review,we discuss the main topics of recent cancer studies that have implemented singlecell RNA sequencing(scRNA-seq).To study cancer cells,scRNA-seq has provided novel insights into the cancer stem-cell model,treatment resistance,and cancer metastasis.To study the tumor microenvironment,scRNA-seq has portrayed the diverse cell types and complex cellular states of both immune and non-immune cells interacting with cancer cells,with the promise to discover novel targets for future immunotherapy.