The prevalence of PMLE varies from 5% to 15% in various studies across the world. PMLE is noticed in the first three decades of life. The mean age in females is 33 years whereas in males it is 35 years. Females are mo...The prevalence of PMLE varies from 5% to 15% in various studies across the world. PMLE is noticed in the first three decades of life. The mean age in females is 33 years whereas in males it is 35 years. Females are more often affected than males. PMLE appears to be an immunologically mediated response possibly a delayed hypersensitivity phenomenon to a photo antigen induced or up regulated in the skin after sun exposure. One hundred new PMLE cases were included in a two year period from 2006-8. All patients, who had been on treatment and with concomitant dermatoses were excluded. On histo-pathological examination 68% showed diagnostic features, 20% showed grade-II features and 12% grade-III features. Histo-pathological grading also varied with the duration of the disease. Early lesions showed only grade-II and III picture whereas the diagnostic picture was evident with the longer duration of the disease. In the plaque type, 14 cases showed characteristic histology after three to four months. In conclusion PMLE is commonly observed in females between 21 - 30 years of age. Pruritus is the chief complaint and the time required for the development of rash increases with time of sun exposure. Most common morphological type is papule on the forearm. Papules and plaque type of lesions of PMLE of longer duration clinched the histo-pathological diagnosis.展开更多
Backgrounds: Pseudomonas aeruginosa is a classic opportunistic pathogen with innate resistance to many antibiotics and disinfectants. The lung is a main target for colonization and infection by the bacteria either in ...Backgrounds: Pseudomonas aeruginosa is a classic opportunistic pathogen with innate resistance to many antibiotics and disinfectants. The lung is a main target for colonization and infection by the bacteria either in the context of a chronic, progressively deteriorating infectious and inflammatory pulmonary disease such as cystic fibrosis (CF) or in a more acute setting such as severe pneumonia in immunocompromised patients [1]. Aim and Objectives: To study the prevalence, virulence and the resistance pattern, phenotypic and genotypic characterization of P. aeruginosa from sputum samples. Materials and Methods: The present study was carried out with a total of 500 clinical sputum samples, which were received from patients, admitted to the various departments of Rajah Muthiah Medical College & Hospital, Annamalai University, Chidambaram. Result: Of the 500 samples subjected for isolation and identification of P. aeruginosa, 116 (23.20%) were positive. The isolated strains were tested for antibiotic sensitivity patterns. 93.10% of P. aeruginosa showed a maximum sensitivity to Ofloxacin, Norfloxacin and 86.20% of strains were highly resistant to Cefotaxime. The same isolates were also tested for phenotypic characterization of Extended Spectrum of Beta Lactamases by double disc synergy method against Cefotaxime and Clavulanic acid, according to the criteria of Hi-Media [2]. Of the resistant strains of P. aeruginosa isolated from sputum, 59% were positive for ESBL. The genotype characterization of ESBL P. aeruginosa showed 40% of CTX-M and 46.66% SHV gene. Conclusion: The present study strongly recommends for further checking of the antibiotic resistant strains of P. aeruginosa for phenotypic characterization of ESBL for effective treatment.展开更多
文摘The prevalence of PMLE varies from 5% to 15% in various studies across the world. PMLE is noticed in the first three decades of life. The mean age in females is 33 years whereas in males it is 35 years. Females are more often affected than males. PMLE appears to be an immunologically mediated response possibly a delayed hypersensitivity phenomenon to a photo antigen induced or up regulated in the skin after sun exposure. One hundred new PMLE cases were included in a two year period from 2006-8. All patients, who had been on treatment and with concomitant dermatoses were excluded. On histo-pathological examination 68% showed diagnostic features, 20% showed grade-II features and 12% grade-III features. Histo-pathological grading also varied with the duration of the disease. Early lesions showed only grade-II and III picture whereas the diagnostic picture was evident with the longer duration of the disease. In the plaque type, 14 cases showed characteristic histology after three to four months. In conclusion PMLE is commonly observed in females between 21 - 30 years of age. Pruritus is the chief complaint and the time required for the development of rash increases with time of sun exposure. Most common morphological type is papule on the forearm. Papules and plaque type of lesions of PMLE of longer duration clinched the histo-pathological diagnosis.
文摘Backgrounds: Pseudomonas aeruginosa is a classic opportunistic pathogen with innate resistance to many antibiotics and disinfectants. The lung is a main target for colonization and infection by the bacteria either in the context of a chronic, progressively deteriorating infectious and inflammatory pulmonary disease such as cystic fibrosis (CF) or in a more acute setting such as severe pneumonia in immunocompromised patients [1]. Aim and Objectives: To study the prevalence, virulence and the resistance pattern, phenotypic and genotypic characterization of P. aeruginosa from sputum samples. Materials and Methods: The present study was carried out with a total of 500 clinical sputum samples, which were received from patients, admitted to the various departments of Rajah Muthiah Medical College & Hospital, Annamalai University, Chidambaram. Result: Of the 500 samples subjected for isolation and identification of P. aeruginosa, 116 (23.20%) were positive. The isolated strains were tested for antibiotic sensitivity patterns. 93.10% of P. aeruginosa showed a maximum sensitivity to Ofloxacin, Norfloxacin and 86.20% of strains were highly resistant to Cefotaxime. The same isolates were also tested for phenotypic characterization of Extended Spectrum of Beta Lactamases by double disc synergy method against Cefotaxime and Clavulanic acid, according to the criteria of Hi-Media [2]. Of the resistant strains of P. aeruginosa isolated from sputum, 59% were positive for ESBL. The genotype characterization of ESBL P. aeruginosa showed 40% of CTX-M and 46.66% SHV gene. Conclusion: The present study strongly recommends for further checking of the antibiotic resistant strains of P. aeruginosa for phenotypic characterization of ESBL for effective treatment.
