The present study reported the morpho-biochemical evaluation of 15 selected rice genotypes for salt tolerance at the seedling stage. Growth parameters including shoot length, root length, plant biomass, plant turgid w...The present study reported the morpho-biochemical evaluation of 15 selected rice genotypes for salt tolerance at the seedling stage. Growth parameters including shoot length, root length, plant biomass, plant turgid weight, plant dry weight along with relative water content were measured after exposure to saline solution （with electrical conductivity value of 12 dS/m）. Genotypes, showing significant differential responses towards salinity in the fields, were assessed through 14 salinity-linked morpho-biochemical attributes, measured at 14 d after exposure of seedling in saline nutrient solution. Relative water content, chlorophyll a/b, peroxidase activity and plant biomass were identified as potential indicators of salt tolerance. Principal component analysis and successive Hierarchical clustering using Euclidean distance revealed that Talmugur, Gheus, Ghunsi, Langalmura, Sabitapalui, and Sholerpona were promising genotypes for further breeding programmes in rice. The maximum Euclidean distance was plotted between Thavallakanan and Talmugur （7.49）, followed by Thavallakanan and Langalmura （6.82）, indicating these combinations may be exploited as parental lines in hybridization programmes to develop salinity tolerant variety.展开更多
Tea is one of the most widely consumed beverages in the world. Black tea, obtained from the leaves of Camellia sinensis is the preferred beverage in India and in most western countries. Epidemiological studies on blac...Tea is one of the most widely consumed beverages in the world. Black tea, obtained from the leaves of Camellia sinensis is the preferred beverage in India and in most western countries. Epidemiological studies on black tea and cancer are limited. However, preliminary studies indicate a positive correlation between black tea consumption and a lower incidence of breast and ovarian cancer. In the present communication, we wanted to see the effect of black tea extract and the polyphenol theaflavin on cell-ECM interaction, MMP activity etc. to strengthen the anti-cancer effect of black tea.展开更多
The present study aimed to detect comparative expression of integrin αVβ3 and its involvement in expression and activation of matrix metalloproteinase-2 (MMP-2) in 25 malignant human breast tumor and adjacent normal...The present study aimed to detect comparative expression of integrin αVβ3 and its involvement in expression and activation of matrix metalloproteinase-2 (MMP-2) in 25 malignant human breast tumor and adjacent normal breast tissues from different clinical TNM stages (DCIS to T4) of the disease and possible involvement of known regulating parameters of MMP-2 like TIMP-2, MT1-MMP and EMPRIN. Integrin αVβ3 was highly expressed in tumors than adjacent normal breast tissues. Pro-MMP-2(72-KD) was mainly expressed in adjacent normal tissues compared to tumors. The mature forms of MMP-2 (68 KD and 64 KD) were found only in tumors. Appreciable expression of TIMP-2 and induction of MT1-MMP and EMPRIN in T2-T4 stages suggested their possible role in MMP-2 activation. Over expression Integrin αVβ3 in tumors than adjacent normal breast tissues was an indication of cancer progression with involvement of integrin signaling. We conclude that, the co-precipitation of MMP-2 with αvβ3 by anti-αv antibody is a strong indication that integrin αvβ3 is a surface receptor for MMP-2 and αvβ3-MMP-2 complex on the surface of tumor cells may play a very important role in determining the invasive property and malignant behavior of tumor tissues. The positive expression of endogenous inhibitor of MMP-2, TIMP-2 may have an appreciable role in activation of this protease and risk of malignancy in advanced stage of the disease. The enhanced expression of MT1-MMP and EMPRIN suggested a role for these factors in gelatinase regulation. However the exact mechanism(s) remains to be investigated. Finally, evaluation of integrin αVβ3 associated MMP-2 expression and activity may add valuable information and can possibly be therapeutic target. The clinical exploitation of integrins will provide oncologists with novel therapeutic strategies for the treatment of malignancy in breast cancer.展开更多
Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI)that poses a serious threat to the environment and human’s wellbeing...Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI)that poses a serious threat to the environment and human’s wellbeing.The present study were made to isolate and characterize chromium tolerant bacteria in the samples collected from four different plots of Bantala Tannery,Kolkata,West Bengal,India.Pure chromium tolerant bacterial strains were isolated from the tannery sludge samples and their relative MIC(Minimum Inhibitory Concentration)were recorded at different concentrations of Cr(VI)salts to select the highest chromium tolerant bacterium.The selected bacterium was further taken for their growth studies followed by different cultural,morphological and molecular analysis(16S rDNA).The bacterial strain was further studied through SEM(Scanning Electron microscopy)and EDX(Energy Dispersive X-ray)spectroscopy which revealed that TW4 was a gram positive,rod shaped,endospore forming,pleomorphic bacterium with phylogenetic similarities with Isoptericola sp.and genebank accession number SUB1732465 TW4 KX640927.展开更多
Our research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica(Burm. Fil.) Merrill.Most effective encapsulation of in vitro nodal segments [(4 ± 1) mm lo...Our research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica(Burm. Fil.) Merrill.Most effective encapsulation of in vitro nodal segments [(4 ± 1) mm long], ensuing in sphere-shaped artificial seeds of similar morphology, was achieved through 75 mmol·L^(-1)calcium chloride(CaCl_2 · 2H_2O) plus 3%(w/v) Na-alginate with 93.3% conversion frequency. The earliest conversion(within 7 days of incubation) of artificial seeds occurred in half-strength liquid Murashige and Skoog medium. Among the three different temperature regimes [(5 ± 1) °C,(15 ± 1) °C, and(25 ± 1) °C], storage of artificial seeds at(15 ± 1) °C executed the highest frequency of conversion(90%) after 15 days of storage. Lengthier storage significantly reduced the conversion frequency of artificial seeds irrespective of storage temperature. Nevertheless, the conversion frequency after 30 days of storage at(15 ± 1) °C was recorded at 70% without further decline even following45 days of storage, which evidently suggests that lower temperature(15 ± 1) °C is apt for storage and subsequent conversion of T. indica artificial seeds. The present protocol could be expedient for short-term storing and swapping of T. indica germplasms between national and international laboratories.展开更多
文摘The present study reported the morpho-biochemical evaluation of 15 selected rice genotypes for salt tolerance at the seedling stage. Growth parameters including shoot length, root length, plant biomass, plant turgid weight, plant dry weight along with relative water content were measured after exposure to saline solution （with electrical conductivity value of 12 dS/m）. Genotypes, showing significant differential responses towards salinity in the fields, were assessed through 14 salinity-linked morpho-biochemical attributes, measured at 14 d after exposure of seedling in saline nutrient solution. Relative water content, chlorophyll a/b, peroxidase activity and plant biomass were identified as potential indicators of salt tolerance. Principal component analysis and successive Hierarchical clustering using Euclidean distance revealed that Talmugur, Gheus, Ghunsi, Langalmura, Sabitapalui, and Sholerpona were promising genotypes for further breeding programmes in rice. The maximum Euclidean distance was plotted between Thavallakanan and Talmugur （7.49）, followed by Thavallakanan and Langalmura （6.82）, indicating these combinations may be exploited as parental lines in hybridization programmes to develop salinity tolerant variety.
文摘Tea is one of the most widely consumed beverages in the world. Black tea, obtained from the leaves of Camellia sinensis is the preferred beverage in India and in most western countries. Epidemiological studies on black tea and cancer are limited. However, preliminary studies indicate a positive correlation between black tea consumption and a lower incidence of breast and ovarian cancer. In the present communication, we wanted to see the effect of black tea extract and the polyphenol theaflavin on cell-ECM interaction, MMP activity etc. to strengthen the anti-cancer effect of black tea.
文摘The present study aimed to detect comparative expression of integrin αVβ3 and its involvement in expression and activation of matrix metalloproteinase-2 (MMP-2) in 25 malignant human breast tumor and adjacent normal breast tissues from different clinical TNM stages (DCIS to T4) of the disease and possible involvement of known regulating parameters of MMP-2 like TIMP-2, MT1-MMP and EMPRIN. Integrin αVβ3 was highly expressed in tumors than adjacent normal breast tissues. Pro-MMP-2(72-KD) was mainly expressed in adjacent normal tissues compared to tumors. The mature forms of MMP-2 (68 KD and 64 KD) were found only in tumors. Appreciable expression of TIMP-2 and induction of MT1-MMP and EMPRIN in T2-T4 stages suggested their possible role in MMP-2 activation. Over expression Integrin αVβ3 in tumors than adjacent normal breast tissues was an indication of cancer progression with involvement of integrin signaling. We conclude that, the co-precipitation of MMP-2 with αvβ3 by anti-αv antibody is a strong indication that integrin αvβ3 is a surface receptor for MMP-2 and αvβ3-MMP-2 complex on the surface of tumor cells may play a very important role in determining the invasive property and malignant behavior of tumor tissues. The positive expression of endogenous inhibitor of MMP-2, TIMP-2 may have an appreciable role in activation of this protease and risk of malignancy in advanced stage of the disease. The enhanced expression of MT1-MMP and EMPRIN suggested a role for these factors in gelatinase regulation. However the exact mechanism(s) remains to be investigated. Finally, evaluation of integrin αVβ3 associated MMP-2 expression and activity may add valuable information and can possibly be therapeutic target. The clinical exploitation of integrins will provide oncologists with novel therapeutic strategies for the treatment of malignancy in breast cancer.
文摘Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI)that poses a serious threat to the environment and human’s wellbeing.The present study were made to isolate and characterize chromium tolerant bacteria in the samples collected from four different plots of Bantala Tannery,Kolkata,West Bengal,India.Pure chromium tolerant bacterial strains were isolated from the tannery sludge samples and their relative MIC(Minimum Inhibitory Concentration)were recorded at different concentrations of Cr(VI)salts to select the highest chromium tolerant bacterium.The selected bacterium was further taken for their growth studies followed by different cultural,morphological and molecular analysis(16S rDNA).The bacterial strain was further studied through SEM(Scanning Electron microscopy)and EDX(Energy Dispersive X-ray)spectroscopy which revealed that TW4 was a gram positive,rod shaped,endospore forming,pleomorphic bacterium with phylogenetic similarities with Isoptericola sp.and genebank accession number SUB1732465 TW4 KX640927.
文摘Our research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica(Burm. Fil.) Merrill.Most effective encapsulation of in vitro nodal segments [(4 ± 1) mm long], ensuing in sphere-shaped artificial seeds of similar morphology, was achieved through 75 mmol·L^(-1)calcium chloride(CaCl_2 · 2H_2O) plus 3%(w/v) Na-alginate with 93.3% conversion frequency. The earliest conversion(within 7 days of incubation) of artificial seeds occurred in half-strength liquid Murashige and Skoog medium. Among the three different temperature regimes [(5 ± 1) °C,(15 ± 1) °C, and(25 ± 1) °C], storage of artificial seeds at(15 ± 1) °C executed the highest frequency of conversion(90%) after 15 days of storage. Lengthier storage significantly reduced the conversion frequency of artificial seeds irrespective of storage temperature. Nevertheless, the conversion frequency after 30 days of storage at(15 ± 1) °C was recorded at 70% without further decline even following45 days of storage, which evidently suggests that lower temperature(15 ± 1) °C is apt for storage and subsequent conversion of T. indica artificial seeds. The present protocol could be expedient for short-term storing and swapping of T. indica germplasms between national and international laboratories.
