Objective:To find out the extent of duffy-binding-like(DBL) a gene diversity and the resetting potential of the parasite population in association with severe malaria.Methods:Genotyping of DBLαdomain was done by PCR ...Objective:To find out the extent of duffy-binding-like(DBL) a gene diversity and the resetting potential of the parasite population in association with severe malaria.Methods:Genotyping of DBLαdomain was done by PCR using three sets of primers(FR,F1R2 and F2R2) and the rosetting frequency was assessed by parasite culture followed by ethidium bromide staining and visualization under a fluorescent microscope.Results:The significant association of high parasite density with severe malaria and the positive correlation between rosetting frequency and parasite density in vivo(P = 0.613,P【0.0001) were observed.Moreover,the parasite strains having multiple fragments of F2R2 region and’b’variant of FR region of DBL 1-αshowed increased rosetting frequency and supported the strain specific association of disease severity. Conclusions:The findings suggest that rosetting mediated higher parasitemia might have contributed to the development of severe disease.As the rosetting domain of Plasmodium falciparum erythrocyte membrane protein 1(HEMP1),the DBL a binds to multiple host receptors; the significant association of multiple fragments of F2R2 region with severe malaria suggests several receptor-ligand interactions as the possible mechanisms of pathogenesis.Alternatively, the high percentage distribution of smaller fragments with mild malaria suggests the lack of adequate rosetting epitopes that might have contributed to low rosetting frequency in mild malaria.展开更多
The aim of the study was to investigate the vibriocidal activity of crude extracts of 18 ethnomedicinal plants used by Nicobarese tribe. Plants were collected from the villages of Car Nicobar Island with the help of T...The aim of the study was to investigate the vibriocidal activity of crude extracts of 18 ethnomedicinal plants used by Nicobarese tribe. Plants were collected from the villages of Car Nicobar Island with the help of TKPs （traditional knowledge practitioners）. The methanol extracts were obtained by cold percolation method and the antimicrobial activity of the extracts was observed by agar well diffusion method. Results indicated that out of 18 plants, seven plants exhibited vibriocidal activity. Morinda citrifolia, Ganophyllumfalcatum and Leea aequata were most active among the plants tested. This study thus can be further utilized to formulate the new antimicrobial agents to fight against Vibrio cholerae.展开更多
文摘Objective:To find out the extent of duffy-binding-like(DBL) a gene diversity and the resetting potential of the parasite population in association with severe malaria.Methods:Genotyping of DBLαdomain was done by PCR using three sets of primers(FR,F1R2 and F2R2) and the rosetting frequency was assessed by parasite culture followed by ethidium bromide staining and visualization under a fluorescent microscope.Results:The significant association of high parasite density with severe malaria and the positive correlation between rosetting frequency and parasite density in vivo(P = 0.613,P【0.0001) were observed.Moreover,the parasite strains having multiple fragments of F2R2 region and’b’variant of FR region of DBL 1-αshowed increased rosetting frequency and supported the strain specific association of disease severity. Conclusions:The findings suggest that rosetting mediated higher parasitemia might have contributed to the development of severe disease.As the rosetting domain of Plasmodium falciparum erythrocyte membrane protein 1(HEMP1),the DBL a binds to multiple host receptors; the significant association of multiple fragments of F2R2 region with severe malaria suggests several receptor-ligand interactions as the possible mechanisms of pathogenesis.Alternatively, the high percentage distribution of smaller fragments with mild malaria suggests the lack of adequate rosetting epitopes that might have contributed to low rosetting frequency in mild malaria.
文摘The aim of the study was to investigate the vibriocidal activity of crude extracts of 18 ethnomedicinal plants used by Nicobarese tribe. Plants were collected from the villages of Car Nicobar Island with the help of TKPs （traditional knowledge practitioners）. The methanol extracts were obtained by cold percolation method and the antimicrobial activity of the extracts was observed by agar well diffusion method. Results indicated that out of 18 plants, seven plants exhibited vibriocidal activity. Morinda citrifolia, Ganophyllumfalcatum and Leea aequata were most active among the plants tested. This study thus can be further utilized to formulate the new antimicrobial agents to fight against Vibrio cholerae.
