Effect of electroacupuncture on the expression of CGRP and 5-HT in the serum and colon of IBS-C rats

Objective:To investigate the effects of electricity on the expression of procalcitonin(CGRP)and serotonin(5-HT)in serum and colon in rats with constipation-type irritable bowel syndrome(IBS-C).Methods:50 rats were randomly divided into three groups:normal control group,model group,electroacupuncture group with four single points(tianshu electroacupuncture group,Colonic acupuncture group,Quchi electroacupuncture group,Shangjuxu acupoint group,and bilateral acupoint alternating between left and right),and non-meridian non-acupoint group(non-meridian non-acupoint group on both hip of electroacupuncture rats).The IBS-C model was replicated in the other three groups,except the normal control group,using the cold-fear-starvation synthesis method.After successful replication of the model,the four electroacupuncture single-point group and the non-acupoint group began electroacupuncture treatment once a day for 3 consecutive days.The visceral sensitivity and the expression of CGRP and 5-HT in serum and colon were observed.Results:Compared with the normal control group,the minimum volume threshold of abdominal retractile reflex(AWR)in the model group decreased(P<0.05),and the expressions of 5-HT and CGRP in serum and colon increased(P<0.05).Compared with the model group,AWR minimum volume threshold was increased in the electroacupuncture group with four single acupuncture points(P<0.05),the expression of 5-ht and CGRP in serum and colon was significantly decreased(P<0.05),and there was no significant change in each indicator in the non-acupuncture point group(P>0.05).Conclusion:Electroacupuncture combined with four single points can effectively improve the visceral sensitivity of IBS-C rats and regulate the levels of 5-HT and CGRP in serum and colon of rats,thus providing experimental basis for the clinical treatment of IBS-C.