Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

Effect mechanism of seepage force on the hydraulic fracture propagation

The flow of fluid through the porous matrix of a reservoir rock applies a seepage force to the solid rock matrix.Although the seepage force exerted by fluid flow through the porous matrix of a reservoir rock has a notable influence on rock deformation and failure,its effect on hydraulic fracture(HF)propagation remains ambiguous.Therefore,in this study,we improved a traditional fluid–solid coupling method by incorporating the role of seepage force during the fracturing fluid seepage,using the discrete element method.First,we validated the simulation results of the improved method by comparing them with an analytical solution of the seepage force and published experimental results.Next,we conducted numerical simulations in both homogeneous and heterogeneous sandstone formations to investigate the influence of seepage force on HF propagation.Our results indicate that fluid viscosity has a greater impact on the magnitude and extent of seepage force compared to injection rate,and that lower viscosity and injection rate correspond to shorter hydraulic fracture lengths.Furthermore,seepage force influences the direction of HF propagation,causing HFs to deflect towards the side of the reservoir with weaker cementation and higher permeability.

A novel triple responsive smart fluid for tight oil fracturing-oil expulsion integration

The traditional multi-process to enhance tight oil recovery based on fracturing and huff-n-puff has obvious deficiencies,such as low recovery efficiency,rapid production decline,high cost,and complexity,etc.Therefore,a new technology,the so-called fracturing-oil expulsion integration,which does not need flowback after fracturing while making full use of the fracturing energy and gel breaking fluids,are needed to enable efficient exploitation of tight oil.A novel triple-responsive smart fluid based on“pseudo-Gemini”zwitterionic viscoelastic surfactant(VES)consisting of N-erucylamidopropyl-N,N-dimethyl-3-ammonio-2-hydroxy-1-propane-sulfonate(EHSB),N,N,N′,N′-tetramethyl-1,3-propanediamine(TMEDA)and sodium p-toluenesulfonate(NaPts),is developed.Then,the rheology of smart fluid is systematically studied at varying conditions(CO_(2),temperature and pressure).Moreover,the mechanism of triple-response is discussed in detail.Finally,a series of fracturing and spontaneous imbibition performances are systematically investigated.The smart fluid shows excellent CO_(2)-,thermal-,and pressure-triple responsive behavior.It can meet the technical requirement of tight oil fracturing construction at 140°C in the presence of 3.5 MPa CO_(2).The gel breaking fluid shows excellent spontaneous imbibition oil expulsion(∼40%),salt resistance(1.2×104 mg/L Na+),temperature resistance(140°C)and aging stability(30 days).

The Calculation of Heating Radius and Determination of Parameters in Heavy Oil Steam Stimulation

在稠油吞吐过程中,加热半径是热采中的重要指标,对注汽参数和生产制度起到了指导性作用。运用能量守恒原理,从热量注入方面考虑了蒸汽相变释放的气化潜热,从热量损失方面考虑井筒热量损失和顶底盖层热量损失,最终得到了新的加热半径计算公式。计算结果表明,加热半径受注汽参数、焖井时间、储层参数等因素影响,并系统阐述了岩层各物性参数的计算及选择方法。最后通过实例验证了该方法的计算值和试井解释出的值较为相符,为现场实际应用提供了可靠的方法。

The collision experiment between rolling stones of different shapes and protective cushion in open-pit mines

Though gravel cushions are used worldwide in open-pit mines and railway slopes to control the impact of rolling stones,no universal technical standards have been put in place to guide engineers in their correct design,and few laboratory test results are available with which to characterize collisions between rolling stones and a gravel cushion.We carried out a large number of experiments in which rolling stones made of the same material but differently shaped were dropped from various heights onto cushions with various particle sizes and thicknesses.We investigated the characteristics of the resulting collisions,and the relationships between coefficients of restitution(CORs)of blocks with different shape and release height H,cushion thickness h and particle diameter d are obtained through linear fitting method.Orthogonal testing reveals the relative influence of block shape,release height,and the particle size and thickness of the cushion on the collision characteristics,which can assist engineers in designing a gravel cushion suitable to the distribution and weathering characteristics of rolling stones in a specific area.

Eco-budget-A Model of Urban Environmental Management

In this paper, a new model of urban environmental resource management is introduced. The article analyzes the gap between urban environmental management and the management of economy and human resources. The significance, the key points, the implementation procedures, and steps of eco-budget cycle are discussed.

Hypoglycemic effect of Moringa oleifera leaf extract and its mechanism prediction based on network pharmacology

Diabetes mellitus(DM)is a chronic metabolic disease characterized by hyperglycemia,which poses a serious threat to human health.Moringa oleifera Lam.is a medicinal and edible plant with various physiological functions.However,its main hypoglycemic components and mechanisms are still unclear.In this study,network pharmacology and bioinformatics were used to analyze the potential bioactive substances of M.oleifera leaf extract(MOLE)and its hypoglycemic mechanism.Studies have shown that MOLE has the effect of increasing glucose consumption in insulin resistant-HepG2 cells.MOLE was found to contain 975 compounds by ultrahigh performance liquid chromatography-mass spectrometry(UHPLC-MS).Network pharmacology analysis indicated that the main active component was robinetin and the identified core genes were AKT1 and GAPDH.KEGG pathway enrichment analysis showed that the hypoglycemic effect of MOLE may be closely related to the PI3K-Akt signaling pathway.This study revealed the possible active components and mechanisms of action of M.oleifera for hypoglycemia,laying the theoretical foundation for subsequent studies.

A quantitative study of the scale and distribution of tight gas reservoirs in the Sulige gas field, Ordos Basin, northwest China

Gas and water distribution is discontinuous in tight gas reservoirs,and a quantitative understanding of the factors controlling the scale and distribution of effective reservoirs is important for natural gas exploration.We used geological and geophysical explanation results,dynamic and static well test data,interference well test and static pressure test to calculate the distribution and characteristics of tight gas reservoirs in the H_(8) Member of the Shihezi Formation,Sulige gas field,Ordos Basin,northwest China.Our evaluation system examines the scale,physical properties,gas-bearing properties,and other reservoir features,and results in classification of effective reservoirs into types Ⅰ,Ⅱ,and Ⅲ that differ greatly in size,porosity,permeability,and saturation.The average thickness,length,and width of type Ⅰ effective reservoirs are 2.89,808,and 598 m,respectively,and the porosity is>10.0%,permeability is>1010^(–3)µm^(2),and average gas saturation is>60%.Compared with conventional gas reservoirs,tight gas effective reservoirs are small-scale and have low gas saturation.Our results show that the scale of the sedimentary system controls the size of the dominant microfacies in which tight gas effective reservoirs form.The presence of different types of interbeds hinders the connectivity of effective sand body reservoirs.The gas source conditions and pore characteristics of the reservoirs control sand body gas filling and reservoir formation.The physical properties and structural nature of the reservoirs control gas–water separation and the gas contents of effective reservoirs.The results are beneficial for the understanding of gas reservoir distribution in the whole Ordos Basin and other similar basins worldwide.

Will the future of shale reservoirs lie in CO2 geological sequestration?

CO2 geological sequestration in a depleted shale gas reservoir is a promising method to address the global energy crisis as well as to reduce greenhouse gas emissions. Though improvements have been achieved by many researchers, the carbon sequestration and enhanced gas recovery(CS-EGR) in shale formations is still in a preliminary stage. The current research status of CO2 sequestration in shale gas reservoirs with potential EGR is systematically and critically addressed in the paper. In addition, some original findings are also presented in this paper. This paper will shed light on the technology development that addresses the dual problem of energy crisis and environmental degradation.

Investigation and Analysis of the Current Medical Health Service in Rural Areas

In order to know more about current medical health service conditions, the Project Group of Medical Health Security and Fund- raising in Rural China set up by the Development Research Center under the State Council organized a large-scale field investigation in 2004. The investigation did not utilize random samples, but can still reflect to a very great extent the actual medical health conditions at oresent in the rural areas.

贵州特色农产品物流发展模式研究

现代农产品物流发展模式充分体现了大数据的多源、异构、海量、低密度价值和高时效性要求等特征。通过分析贵州省当前特色农产品物流模式及其存在问题,创新大数据情境下优化发展其物流的冷链模式、数据监督模式和虚拟无水港模式等,并提出这些大数据农产品物流模式的若干发展建议。研究成果对促进贵州省农业产业结构调整、转变经济发展方式和助推"黔货出山"等具有较好的实践意义。