The pattern of clinical forms of respiratory tuberculosis in children shows a preponderance of intrathoracic lymph node tuberculosis （89.4%） that is characterized by a complicated process in every third child under ...The pattern of clinical forms of respiratory tuberculosis in children shows a preponderance of intrathoracic lymph node tuberculosis （89.4%） that is characterized by a complicated process in every third child under present-day conditions. Positive result of PCR closely correlates with the severity and extent of the specific process in children. Real-time PCR （RT-PCR） was ascertain to exhibit the highest sensitivity in detecting Mycobacterium tuberculosis DNA in children with primary generalized tuberculosis （62.5%） and in those with a disseminated specific process （55.6%）, which was much higher than conventional bacteriological study of diagnostic materials. By taking into account the findings, the RT-PCR detection of M. tuberculosis was considered as a substantial criterion for evaluating the magnitude of specific changes and the degree of tuberculosis infection activity in children.展开更多
The main problem in treatment of children with various forms of tuberculosis is the lack of pediatric dosages of anti-TB drugs. The aim of the study is to determine efficacy of the 〈Isoniazid 150 mg＋Pyrazinamid 375...The main problem in treatment of children with various forms of tuberculosis is the lack of pediatric dosages of anti-TB drugs. The aim of the study is to determine efficacy of the 〈Isoniazid 150 mg＋Pyrazinamid 375 mg＋Rifampicin 150 mg〉 （Ftizamax ） fixed-dose combination. We estimated the clinical, radiological and laboratory parameters during treatment of 73 children with pulmonary tuberculosis, of which 34 children were treated by the 〈Isoniazid 150 mg＋Pyrazinamid 375 mg＋Rifampicin 150 mg〉 in combination with the Ethambutol; while the others were treated with mono-drugs under the standard regimen I. Effective use of 〈Isoniazid 150 mg＋Pyrazinamid 375 mg＋Rifampicin 150 mg〉 （Ftizamax ） for children is supported by positive results towards the end of the intensive phase of the therapy and the continuation phase, as well as by minimized adverse drug reactions during treatment. Combination of active substances in the drug and its dosage is easy to use, which allows recommending this drug for treatment of tuberculosis in children.展开更多
Despite well-known limitations,mice remain useful as model animals to study tuberculosis(TB)pathogenesis,the basic immune response,the extent of lung pathology as well as efficacy of new drugs against Mycobacterium tu...Despite well-known limitations,mice remain useful as model animals to study tuberculosis(TB)pathogenesis,the basic immune response,the extent of lung pathology as well as efficacy of new drugs against Mycobacterium tuberculosis[1,2].There are four routes of tuberculosis infection in mice:aerosol generation and exposition,intravenous injection,intranasal administration,and subcutaneous administration[3].展开更多
The biocomposite films were prepared from poly(L-lactic acid)and cellulose nanocrystals.To improve interfacial compatibility of hydrophilic cellulose nanocrystals with hydrophobic matrix polymer as well as to provide ...The biocomposite films were prepared from poly(L-lactic acid)and cellulose nanocrystals.To improve interfacial compatibility of hydrophilic cellulose nanocrystals with hydrophobic matrix polymer as well as to provide the osteoconductive properties,cellulose was functionalized with poly(glutamic acid).The modified cellulose nanocrystals were better distributed and less aggregated within the matrix,which was testified by scanning electron,optical and polarized light microscopy.According to mechanical tests,composites filled with nanocrystals modified with PGlu demonstrated higher values of Young’s modulus,elongation at break and tensile strength.Incubation of composite materials in model buffer solutions for 30 weeks followed with staining of Ca^(2+)deposits with Alizarin Red S assay testified better mineralization of materials containing PGlu-modified cellulose nanocrystals as filler.As the result of in vivo experiment,the developed composite materials showed less level of inflammation in comparison with pure polymer matrix and composites filled with non-functionalized cellulose nanocrystals.展开更多
文摘The pattern of clinical forms of respiratory tuberculosis in children shows a preponderance of intrathoracic lymph node tuberculosis （89.4%） that is characterized by a complicated process in every third child under present-day conditions. Positive result of PCR closely correlates with the severity and extent of the specific process in children. Real-time PCR （RT-PCR） was ascertain to exhibit the highest sensitivity in detecting Mycobacterium tuberculosis DNA in children with primary generalized tuberculosis （62.5%） and in those with a disseminated specific process （55.6%）, which was much higher than conventional bacteriological study of diagnostic materials. By taking into account the findings, the RT-PCR detection of M. tuberculosis was considered as a substantial criterion for evaluating the magnitude of specific changes and the degree of tuberculosis infection activity in children.
文摘The main problem in treatment of children with various forms of tuberculosis is the lack of pediatric dosages of anti-TB drugs. The aim of the study is to determine efficacy of the 〈Isoniazid 150 mg＋Pyrazinamid 375 mg＋Rifampicin 150 mg〉 （Ftizamax ） fixed-dose combination. We estimated the clinical, radiological and laboratory parameters during treatment of 73 children with pulmonary tuberculosis, of which 34 children were treated by the 〈Isoniazid 150 mg＋Pyrazinamid 375 mg＋Rifampicin 150 mg〉 in combination with the Ethambutol; while the others were treated with mono-drugs under the standard regimen I. Effective use of 〈Isoniazid 150 mg＋Pyrazinamid 375 mg＋Rifampicin 150 mg〉 （Ftizamax ） for children is supported by positive results towards the end of the intensive phase of the therapy and the continuation phase, as well as by minimized adverse drug reactions during treatment. Combination of active substances in the drug and its dosage is easy to use, which allows recommending this drug for treatment of tuberculosis in children.
文摘Despite well-known limitations,mice remain useful as model animals to study tuberculosis(TB)pathogenesis,the basic immune response,the extent of lung pathology as well as efficacy of new drugs against Mycobacterium tuberculosis[1,2].There are four routes of tuberculosis infection in mice:aerosol generation and exposition,intravenous injection,intranasal administration,and subcutaneous administration[3].
基金funded by the Russian Ministry of Education and Science(state contract no.14.W03.31.0014,MegaGrant).
文摘The biocomposite films were prepared from poly(L-lactic acid)and cellulose nanocrystals.To improve interfacial compatibility of hydrophilic cellulose nanocrystals with hydrophobic matrix polymer as well as to provide the osteoconductive properties,cellulose was functionalized with poly(glutamic acid).The modified cellulose nanocrystals were better distributed and less aggregated within the matrix,which was testified by scanning electron,optical and polarized light microscopy.According to mechanical tests,composites filled with nanocrystals modified with PGlu demonstrated higher values of Young’s modulus,elongation at break and tensile strength.Incubation of composite materials in model buffer solutions for 30 weeks followed with staining of Ca^(2+)deposits with Alizarin Red S assay testified better mineralization of materials containing PGlu-modified cellulose nanocrystals as filler.As the result of in vivo experiment,the developed composite materials showed less level of inflammation in comparison with pure polymer matrix and composites filled with non-functionalized cellulose nanocrystals.
